Understanding fungal potential in the mitigation of contaminated areas in the Czech Republic: tolerance, biotransformation of hexachlorocyclohexane (HCH) and oxidative stress analysis

Russo, Fabiana; Ceci, Andrea; Maggi, Oriana; Siciliano, Antonietta; Guida, Marco; Papini, Marco Petrangeli; Černík, Miroslav; Persiani, Anna Maria

doi:10.1007/s11356-019-05679-w

Cited by 9 publications

(6 citation statements)

References 111 publications

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“…By comparing the results of treatment to the control, the divergent response in CAT and SOD activity in R. arrhizus was seen to be consistent with those concerning the effect on these enzymes by different pesticides in other fungal species (82). In Russo et al (92), ROS production and antioxidant enzyme activities were investigated for two fungal species in the presence of HCH isomers. These researchers observed an increase in ROS production and CAT and GST activities in both of the tested fungi: in Penicillium simplicissimum the SOD activity significantly increased under HCH stress conditions compared to the control, whereas in T. harzianum the SOD activity did not show the same trend (92).…”

Section: Figsupporting

confidence: 54%

“…In Russo et al (92), ROS production and antioxidant enzyme activities were investigated for two fungal species in the presence of HCH isomers. These researchers observed an increase in ROS production and CAT and GST activities in both of the tested fungi: in Penicillium simplicissimum the SOD activity significantly increased under HCH stress conditions compared to the control, whereas in T. harzianum the SOD activity did not show the same trend (92). As reported by Kreiner et al (82), exposure to menadione (MD) in Aspergillus niger also first resulted in an increase in CAT activity, whereas the SOD activity did not increase.…”

Section: Figmentioning

confidence: 99%

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Bioremediation of Dichlorodiphenyltrichloroethane (DDT)-Contaminated Agricultural Soils: Potential of Two Autochthonous Saprotrophic Fungal Strains

Russo

Ceci

Pinzari

et al. 2019

Appl Environ Microbiol

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DDT (dichlorodiphenyltrichloroethane) was used worldwide as an organochlorine insecticide to control agricultural pests and vectors of several insect-borne human diseases. It was banned in most industrialized countries; however, due to its persistence in the environment, DDT residues remain in environmental compartments, becoming long-term sources of exposure. To identify and select fungal species suitable for bioremediation of DDT-contaminated sites, soil samples were collected from DDT-contaminated agricultural soils in Poland, and 38 fungal taxa among 18 genera were isolated. Two of them, Trichoderma hamatum FBL 587 and Rhizopus arrhizus FBL 578, were tested for tolerance in the presence of 1-mg liter−1 DDT concentration by using two indices based on fungal growth rate and biomass production (the tolerance indices Rt:Rc and TI), showing a clear tolerance to DDT. The two selected strains were studied to evaluate catabolic versatility on 95 carbon sources with or without DDT by using the Phenotype MicroArray system and to investigate the induced oxidative stress responses. The two strains were able to use most of the substrates provided, resulting in both high metabolic versatility and ecological functionality in the use of carbon sources, despite the presence of DDT. The activation of specific metabolic responses with species-dependent antioxidant enzymes to cope with the induced chemical stress has been hypothesized, since the presence of DDT promoted a higher formation of reactive oxygen species in fungal cells than the controls. The tested fungi represent attractive potential candidates for bioremediation of DDT-contaminated soil and are worthy of further investigations. IMPORTANCE The spread and environmental accumulation of DDT over the years represent not only a threat to human health and ecological security but also a major challenge because of the complex chemical processes and technologies required for remediation. Saprotrophic fungi, isolated from contaminated sites, hold promise for their bioremediation potential toward toxic organic compounds, since they might provide an environment-friendly solution to contamination. Once we verified the high tolerance of autochthonous fungal strains to high concentrations of DDT, we showed how fungi from different phyla demonstrate a high metabolic versatility in the presence of DDT. The isolates showed the singular ability to keep their functionality, despite the DDT-induced production of reactive oxygen species.

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Section: Figsupporting

confidence: 54%

Section: Figmentioning

confidence: 99%

Bioremediation of Dichlorodiphenyltrichloroethane (DDT)-Contaminated Agricultural Soils: Potential of Two Autochthonous Saprotrophic Fungal Strains

Russo

Ceci

Pinzari

et al. 2019

Appl Environ Microbiol

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“…Till now, bacterial or fungal (Russo et al 2019 ) strains have been usually applied for bioremediation of polluted areas. Soil yeasts have not been used to the same extent because they tend to have a strong endemism and there are a surprisingly high number of currently unidentified species (Yurkov 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Moniliella spathulata, an oil-degrading yeast, which promotes growth of barley in oil-polluted soil

Mikolasch¹,

Berzhanova

Omirbekova

et al. 2020

Appl Microbiol Biotechnol

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The yeast strain Moniliella spathulata SBUG-Y 2180 was isolated from oil-contaminated soil at the Tengiz oil field in the Atyrau region of Kazakhstan on the basis of its unique ability to use crude oil and its components as the sole carbon and energy source. This yeast used a large number of hydrocarbons as substrates (more than 150), including n-alkanes with chain lengths ranging from C10 to C32, monomethyl- and monoethyl-substituted alkanes (C9–C23), and n-alkylcyclo alkanes with alkyl chain lengths from 3 to 24 carbon atoms as well as substituted monoaromatic and diaromatic hydrocarbons. Metabolism of this huge range of hydrocarbon substrates produced a very large number of aliphatic, alicyclic, and aromatic acids. Fifty-one of these were identified by GC/MS analyses. This is the first report of the degradation and formation of such a large number of compounds by a yeast. Inoculation of barley seeds with M. spathulata SBUG-Y 2180 had a positive effect on shoot and root development of plants grown in oil-contaminated sand, pointing toward potential applications of the yeast in bioremediation of polluted soils. Key points • Moniliella spathulata an oil-degrading yeast • Increase of the growth of barley

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“…(%) = (DW of treated mycelium/DW of control mycelium) × 100) [23]. To facilitate the recovery of the mycelium, sterile cellophane membranes, allowing the passage of nutrients and metabolites between the medium and the colony, were placed on the surface of the culture medium [22]. Inoculation was carried out placing at the center of the plate a 6-mm-diameter plug taken from the actively growing margin of the stock cultures using a sterile cork borer.…”

Section: Screening Of Fungal Strains For Arsenic Tolerance and Accumu...mentioning

confidence: 99%

“…Most of the above-mentioned tested strains were isolated from contaminated sites such as agricultural soils, paddy fields, and mines mainly in China and India. In order to maximize the efficiency in mycoremediation applications, it is very important to focus on saprotrophic fungi isolated from soils with high As concentrations as they can be better equipped to survive and cope with the associated chemical stress [22,23].…”

Section: Introductionmentioning

confidence: 99%

Fungi and Arsenic: Tolerance and Bioaccumulation by Soil Saprotrophic Species

et al. 2020

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Increasing arsenic environmental concentrations are raising worldwide concern for its impacts on human health and ecosystem functionality. In order to cope with arsenic contamination, bioremediation using fungi can represent an efficient, sustainable, and cost-effective technological solution. Fungi can mitigate arsenic contamination through different mechanisms including bioaccumulation. In this work, four soil saprotrophic fungi Absidia spinosa, Purpureocillium lilacinum, Metarhizium marquandii, and Cephalotrichum nanum, isolated from soils with naturally high arsenic concentrations, were tested for their ability to tolerate different sodium arsenite concentrations and accumulate As in different cultural conditions. pH medium after fungal growth was measured to study pH variation and metabolic responses. Arsenic bioaccumulation and its influence on the uptake of other elements were investigated through multi-elemental analysis using hydride generation atomic fluorescence spectrometry (HG-AFS), inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectroscopy (ICP-OES). Considering the increasing interest in siderophore application for metal bioremediation, the production of siderophores and their affinity for both Fe and As were also evaluated. All species were able to tolerate and accumulate As in their biomass in all of the tested conditions and produced siderophores with different affinities for Fe and As. The results suggest that the tested fungi are attractive potential candidates for the bioremediation of As contaminated soil and worthy of further investigation.

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Understanding fungal potential in the mitigation of contaminated areas in the Czech Republic: tolerance, biotransformation of hexachlorocyclohexane (HCH) and oxidative stress analysis

Cited by 9 publications

References 111 publications

Bioremediation of Dichlorodiphenyltrichloroethane (DDT)-Contaminated Agricultural Soils: Potential of Two Autochthonous Saprotrophic Fungal Strains

Bioremediation of Dichlorodiphenyltrichloroethane (DDT)-Contaminated Agricultural Soils: Potential of Two Autochthonous Saprotrophic Fungal Strains

Moniliella spathulata, an oil-degrading yeast, which promotes growth of barley in oil-polluted soil

Fungi and Arsenic: Tolerance and Bioaccumulation by Soil Saprotrophic Species

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