Understanding electrogenerated chemiluminescence at graphite screen-printed electrodes

Disposable capillary-fill device for the determination of proteases incorporating elimination of light-shielding from the magnetic beads with cleavage of the electrogenerated chemiluminescence label-tagged peptide probe

“…45 However, Ru(bpy) 3 3+ can be only electrogenerated in the electron tunneling (∼3–9 nm) 46 at the surface of the electrode, and the TPrA radicals can be used more efficiently at a relatively higher potential, resulting in a much greater concentration dependence and generating a stronger ECL signal than the lower potential. 38,44…”

“…43 The independence of the ECL emission at ∼1 V on the concentration may be ascribed to the following three reasons: (1) The ECL emission at lower potential, which originates from the chemical reaction between Ru(bpy) 3 + and TPrA •+ , 38 depends much more on the nature of the electrode and electron transfer ability of the luminophore and co-reactant. 44 (2) Electrogenerated insufficient TPrA radicals relative to the concentration of Ru(bpy) 3 2+ at the lower potential, and the ECL signal will be controlled by the concentration of TPrA radicals. 38 (3) The electrogenerated unstable TPrA •+ (lifetime 0.2 ms) was more susceptible to the environmental situation in the homogeneous diffusion layer (∼3-4 μm).…”

“…45 However, Ru (bpy) 3 3+ can be only electrogenerated in the electron tunneling (∼3-9 nm) 46 at the surface of the electrode, and the TPrA radicals can be used more efficiently at a relatively higher potential, resulting in a much greater concentration dependence and generating a stronger ECL signal than the lower potential. 38,44 The selectivity of the developed ECL method on the prepared MB ECL probes using the ECL capillary-fill device was also examined in the presence of possible interferents in human serum samples, including 200 ng mL −1 D-dimer, 24 ng mL −1 alpha-fetoprotein (AFP), 0.40 ng mL −1 cardiac troponin I (cTnI), 0.80 ng mL −1 cardiac troponin T (cTnT) and 200 ng mL −1 myoglobin (Myo). As shown in Fig.…”