Understanding conduction of electrical impulses in the mouse heart using high-resolution video imaging technology

Morley, Gregory E.; Vaidya, Dhananjay

doi:10.1002/1097-0029(20010201)52:3<241::aid-jemt1010>3.0.co;2-3

Cited by 16 publications

(13 citation statements)

References 82 publications

(107 reference statements)

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“…This was possible since the blebbistatin at the concentration necessary to inhibit contractions considerably slowed down the intrinsic heart rate. Imaged area included a square of 8 × 8 mm in the middle of left ventricular free wall (lateral view) where the orientation of the subepicardial layer of cardiomyocytes is predominantly oblique (Morley and Vaidya, 2001). …”

Section: Methodsmentioning

confidence: 99%

Changes in Myocardial Composition and Conduction Properties in Rat Heart Failure Model Induced by Chronic Volume Overload

et al. 2016

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Volume overload leads to development of eccentric cardiac hypertrophy and heart failure. In our previous report, we have shown myocyte hypertrophy with no fibrosis and decrease in gap junctional coupling via connexin43 in a rat model of aorto-caval fistula at 21 weeks. Here we set to analyze the electrophysiological and protein expression changes in the left ventricle and correlate them with phenotypic severity based upon ventricles to body weight ratio. ECG analysis showed increased amplitude and duration of the P wave, prolongation of PR and QRS interval, ST segment elevation and decreased T wave amplitude in the fistula group. Optical mapping showed a prolongation of action potential duration in the hypertrophied hearts. Minimal conduction velocity (CV) showed a bell-shaped curve, with a significant increase in the mild cases and there was a negative correlation of both minimal and maximal CV with heart to body weight ratio. Since the CV is influenced by gap junctional coupling as well as the autonomic nervous system, we measured the amounts of tyrosine hydroxylase (TH) and choline acetyl transferase (ChAT) as a proxy for sympathetic and parasympathetic innervation, respectively. At the protein level, we confirmed a significant decrease in total and phosphorylated connexin43 that was proportional to the level of hypertrophy, and similarly decreased levels of TH and ChAT. Even at a single time-point, severity of morphological phenotype correlates with progression of molecular and electrophysiological changes, with the most hypertrophied hearts showing the most severe changes that might be related to arrhythmogenesis.

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Section: Methodsmentioning

confidence: 99%

Changes in Myocardial Composition and Conduction Properties in Rat Heart Failure Model Induced by Chronic Volume Overload

et al. 2016

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“…Optical mapping data can also be used to generate local conduction vectors, which then allow for the quantification of local conduction velocities as well as providing a description of the direction of activation of the myocardium. A general description of how local CV vectors are calculated is provided below, and more specific details can be found in the original references (4,39) and in a detailed review on imaging electrical wave propagation and conduction (34).…”

Section: Analysis Of Optical Imaging Datamentioning

confidence: 99%

Processing and analysis of cardiac optical mapping data obtained with potentiometric dyes

Laughner

Sulkin

et al. 2012

American Journal of Physiology-Heart and Circulatory Physiology

180

182

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“…Optical imaging of the heart using voltage-sensitive dyes has made it possible to record cardiac APs with high spatial and temporal resolution but the susceptibility of optical imaging to motion artifacts is ever present [4][6] [7]. Conversely, at an individual cell level, the cardiac AP can be measured by a micro-electrode where the occurrence of motion artifacts is limited, but the spatial resolution suffers greatly due to its utilization.…”

Section: A Current State Of the Field And Literature Reviewmentioning

confidence: 99%

“…Data from this previous study [4] is used in this study to develop a method for wavefront tracking. Currently, the techniques used to analyze cardiac imaged data range from determining the overall activation time for the heart to calculating the localized velocity vectors for each step of a propagating wavefront from initial upstroke to rest [4] [7].…”

Section: A Current State Of the Field And Literature Reviewmentioning

confidence: 99%

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Cardiac action potential wavefront tracking using optical mapping

Ashraf

Nygren

2009

2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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A wealth of knowledge is available about the effect of diabetes on the heart but very little has been done to quantify the conduction velocity of the diabetic heart. This study intends to develop a technique for tracking the cardiac wavefront across the heart in order to achieve the total activation time as well as the conduction velocity of the heart at any point during its activation, to compare the newly determined activation times with previously determined activation times, and to also compute the average conduction velocity of the heart from diabetic and control rats. The technique developed for tracking the action potential wavefront across the heart extracts the wavefront and provides the activation time as well as the conduction velocity - both instantaneous and average - successfully. The method reproduces previously measured activation times well, with a correlation of R(2) = 0.875, which suggests that this technique is reliable and that its determination of conduction velocity will allow for the examination of healthy and diseased hearts using a new criterion. In addition, the method for determining the conduction velocity of the heart allows direct comparison of the baseline conduction velocity in control and diabetic hearts. The results of this comparison indicated that conduction velocity in the diabetic hearts is slower (0.47 +/- 0.02 m/s) than in control hearts (0.55 +/- 0.02 m/s) (p = 0.001).

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Understanding conduction of electrical impulses in the mouse heart using high-resolution video imaging technology

Cited by 16 publications

References 82 publications

Changes in Myocardial Composition and Conduction Properties in Rat Heart Failure Model Induced by Chronic Volume Overload

Changes in Myocardial Composition and Conduction Properties in Rat Heart Failure Model Induced by Chronic Volume Overload

Processing and analysis of cardiac optical mapping data obtained with potentiometric dyes

Cardiac action potential wavefront tracking using optical mapping

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