Understanding and manipulating plant lipid composition: Metabolic engineering leads the way

Napier, Johnathan A.; Haslam, Richard P.; Beaudoin, Frédéric; Cahoon, Edgar B.

doi:10.1016/j.pbi.2014.04.001

Cited by 91 publications

(74 citation statements)

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“…Therefore, the bioengineering wileyonlinelibrary.com/journal/pld3 | 1 of oilseed crops to accumulate TAG with novel fatty acid compositions for use in food or industrial feedstocks has been a goal of the plant lipid community for over 20 years. Most engineering of plants to produce novel TAG fatty acid compositions has been first demonstrated in Arabidopsis thaliana seeds, and the wide range of unique fatty acid compositions produced has been reviewed extensively (Aznar-Moreno & Durrett, 2017;Bates, 2016;Cahoon et al, 2007;Carlsson et al, 2011;Dyer et al, 2008;Haslam et al, 2013;Lee, Chen, & Kim, 2015;Lu, Napier, Clemente, & Cahoon, 2011;Napier, 2007;Napier, Haslam, Beaudoin, & Cahoon, 2014;Ruiz-Lopez, Usher, Sayanova, Napier, & Haslam, 2015;Singh, Zhou, Liu, Stymne, & Green, 2005;Vanhercke, Wood, Stymne, Singh, & Green, 2013).Despite the over two decades of plant lipid engineering, we still cannot predict the effect of most engineering approaches on the final fatty acid composition or total oil amount, thereby implying that more basic research is needed to understand the factors which control both wild type and transgenic seed oil content. were also coexpressed with the hydroxylase to selectively accumulate HFA in seed oil (Bates et al, 2014;Burgal et al, 2008;van Erp et al, 2011van Erp et al, , 2015.…”

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“…Most engineering of plants to produce novel TAG fatty acid compositions has been first demonstrated in Arabidopsis thaliana seeds, and the wide range of unique fatty acid compositions produced has been reviewed extensively (Aznar-Moreno & Durrett, 2017;Bates, 2016;Cahoon et al, 2007;Carlsson et al, 2011;Dyer et al, 2008;Haslam et al, 2013;Lee, Chen, & Kim, 2015;Lu, Napier, Clemente, & Cahoon, 2011;Napier, 2007;Napier, Haslam, Beaudoin, & Cahoon, 2014;Ruiz-Lopez, Usher, Sayanova, Napier, & Haslam, 2015;Singh, Zhou, Liu, Stymne, & Green, 2005;Vanhercke, Wood, Stymne, Singh, & Green, 2013).…”

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The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds

Karki

Bates

2018

Plant Direct

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Arabidopsis thaliana is the most developed and utilized model plant. In particular, it is an excellent model for proof-of-concept seed oil engineering studies because it accumulates approximately 37% seed oil by weight, and it is closely related to important Brassicaceae oilseed crops. Arabidopsis can be grown under a wide variety of conditions including continuous light; however, the amount of light is strongly correlated with total seed oil accumulation. In addition, many attempts to engineer novel seed oil fatty acid compositions in Arabidopsis have reported significant reductions in oil accumulation; however, the relative reduction from the nontrans- between transgenic lines and nontransgenic controls is dependent on both the light conditions and the type of oil content measurement utilized. In addition, the light conditions effect the relative accumulation of the novel fatty acids between various transgenic lines. Therefore, the success of novel fatty acid proof-of-concept engineering strategies on both oil accumulation and fatty acid composition in Arabidopsis seeds should be considered in light of the select growth and measurement conditions prior to moving engineering strategies into crop plants. K E Y W O R D Shydroxylated fatty acids, light, ricinoleate, triacylglycerol | INTRODUCTIONThe fatty acids within triacylglycerols (TAGs, oils) are the most energy dense form of biological carbon storage. TAGs which accumulate in the seeds of oilseed crops are an important nutritional source for both calories and essential fatty acids required in human diets. In addition, seed oils also represent a renewable resource for industrial chemicals and biofuels (Carlsson, Yilmaz, Green, Stymne, & Hofvander, 2011;Durrett, Benning, & Ohlrogge, 2008;Dyer, Stymne, Green, & Carlsson, 2008). However, many of the most nutritionally valuable or industrially useful fatty acids do not accumulate in major oilseed crops, but accumulate in microalgae or in terrestrial plants with poor agronomic features (Badami & Patil, 1980;Gunstone, Harwood, & Dijkstra, 2007). Therefore, the bioengineering wileyonlinelibrary.com/journal/pld3 | 1 of oilseed crops to accumulate TAG with novel fatty acid compositions for use in food or industrial feedstocks has been a goal of the plant lipid community for over 20 years. Most engineering of plants to produce novel TAG fatty acid compositions has been first demonstrated in Arabidopsis thaliana seeds, and the wide range of unique fatty acid compositions produced has been reviewed extensively (Aznar-Moreno & Durrett, 2017;Bates, 2016;Cahoon et al., 2007;Carlsson et al., 2011;Dyer et al., 2008;Haslam et al., 2013;Lee, Chen, & Kim, 2015;Lu, Napier, Clemente, & Cahoon, 2011;Napier, 2007;Napier, Haslam, Beaudoin, & Cahoon, 2014;Ruiz-Lopez, Usher, Sayanova, Napier, & Haslam, 2015;Singh, Zhou, Liu, Stymne, & Green, 2005;Vanhercke, Wood, Stymne, Singh, & Green, 2013).Despite the over two decades of plant lipid engineering, we still cannot predict the effect of most engineering approaches on the final f...

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The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds

Karki

Bates

2018

Plant Direct

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“…Oil seeds contain FAs that are primarily in the form of lipids such as triacylglycerol esters (TAGs). TAG accumulation can be increased via the ectopic expression of FA biosynthetic enzymes (di-and mono-acylglycerol acyltransferase) and transcription factor genes (WRINKLED1 and LEAFY COT-YLEDON1), which control seed development, as well as site-directed mutagenesis of COMATOSE and SUGAR DEPENDENT1, functioning in TAG and FA degradation (Napier et al, 2014). Additionally, attempts have been made to use various RNAi and transgenic technologies to alter FA content, but they failed to produce the desirable FA traits or had unusual pleiotropic phenotype effects on oil crops (Shen et al, 2010).…”

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Targeted genome editing, an alternative tool for trait improvement in horticultural crops

Subburaj

Jin

et al. 2016

Hortic. Environ. Biotechnol.

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Abstract. Improving crops through plant breeding, an important approach for sustainable agriculture, has been utilized to increase the yield and quality of foods and other biomaterials for human use. Crops, including cereals, vegetables, ornamental flowers, fruits, and trees, have long been cultivated to produce high-quality products for human consumption. Conventional breeding technologies, such as natural cross-hybridization, mutation induction through physical or chemical mutagenesis, and modern transgenic tools are often used to enhance crop production. However, these breeding methods are sometimes laborious and complicated, especially when attempting to improve desired traits without inducing pleiotropic effects. Recently, targeted genome editing (TGE) technology using engineered nucleases, including meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) nucleases, has been used to improve the traits of economically important plants. TGE has emerged as a novel plant-breeding tool that represents an alternative approach to classical breeding, but with higher mutagenic efficiency. Here, we briefly describe the basic principles of TGE and the types of engineered nucleases utilized, along with their advantages and disadvantages. We also discuss their potential use to improve the traits of horticultural crops through genome engineering.

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“…Camelina has emerged as an attractive host for metabolic engineering because it can be easily transformed using an Agrobacterium-based floral infiltration (Lu and Kang, 2008). Several other characteristics of Camelina contribute to the rising interest in its use: the oil content of camelina ranges from 28 to 40% with storage proteins making up a further 30%; a relatively short life cycle, good performance on marginal land (yields surpass other oilseed crops under drought-like conditions); low requirement for nutrients and a low seeding rate (Nguyen et al, 2013;Napier et al, 2014). To facilitate genetic improvement the genome of camelina has now been sequenced revealing an overall expansion of lipid metabolism gene families (217% compared with Arabidopsis) and suggesting that complex regulatory mechanisms govern oil biosynthesis (Kagale et al, 2014).…”

Section: Metabolic Engineering Of Novel Oil Traits In Camelina Sativamentioning

confidence: 99%

Oleaginous crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock

Beaudoin

Sayanova

Haslam

et al. 2014

OCL

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-The world faces considerable challenges including how to produce more biomass for food, feed, fuel and industrial feedstock without significantly impacting on our environment or increasing our consumption of limited resources such as water or petroleum-derived carbon. This has been described as sustainable intensification. Oleaginous crops have the potential to provide renewable resources for all these commodities, provided they can be engineered to meet end-use requirements, and that they can be produced on sufficient scale to meet current growing world population and industrial demand. Although traditional breeding methods have been used successfully to modify the fatty acid composition of oils, metabolic engineering provides a more rapid and direct method for manipulating plant lipid composition. Recent advances in our understanding of the biochemical mechanisms of seed oil biogenesis and the cloning of genes involved in fatty acid and oil metabolic pathways, have allowed the generation of oilseed crops that produce 'designer oils' tailored for specific applications and the conversion of high biomass crops into novel oleaginous crops. However, improvement of complex quantitative traits in oilseed crops remains more challenging as the underlying genetic determinants are still poorly understood. Technological advances in sequencing and computing have allowed the development of an association genetics method applicable to crops with complex genomes. Associative transcriptomics approaches and high throughput lipidomic profiling can be used to identify the genetic components controlling quantitative variation for lipid related traits in polyploid crops like oilseed rape and provide molecular tools for marker assisted breeding. In this review we are citing examples of traits with potential for bio-refining that can be harvested as co-products in seeds, but also in non-harvested biomass.Keywords: Crops improvement / oleaginous crops / metabolic engineering / designer oil / molecular breeding / Genome Wide Association Studies / associative transcriptomics / co-products Résumé -Les oléagineux en tant que plateformes intégrées de production pour l'alimentation humaine et animale, les carburants et les matières premières industrielles renouvelables : Manipulation de la composition lipidique de la plante par ingénierie métabolique et nouvelles opportunités d'associations génétiques pour l'amé-lioration des cultures et la valorisation des coproduits. Le monde fait face à des défis considérables, incluant le moyen de produire davantage de biomasse pour l'alimentation humaine et animale, les carburants et les matières premières industrielles, et ce sans impact environnemental significatif ou sans accroître notre consommation de ressources limitées comme l'eau ou le carbone dérivé du pétrole. Cela a été décrit comme de l'intensification durable. Les oléa-gineux disposent du potentiel nécessaire pour fournir des ressources renouvelables pour toutes ces matières premières, sous réserve qu'ils puissent être manipulés ...

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Understanding and manipulating plant lipid composition: Metabolic engineering leads the way

Abstract: HighlightsDescription of recent advances in plant lipid metabolism.Description of break-through achievements in plant metabolic engineering.Insights into the practical applications of plant synthetic biology.

Cited by 91 publications

References 45 publications

The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds

The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds

Targeted genome editing, an alternative tool for trait improvement in horticultural crops

Oleaginous crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock

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