Understanding and managing peak shape for basic solutes in reversed-phase high performance liquid chromatography

Abstract

While other separation mechanisms can challenge the dominance of reversed-phase in some applications (for example in the separation of native proteins), reversed-phase liquid chromatography is the method of choice for...

Development and validation of the RP-HPLC method for quantification of tavaborole

“…The chromatographic conditions were optimized, considering factors such as peak symmetry, tailing factor, theoretical plate, and retention time. 18,19 TVB has a partition coefficient of 1.28, indicating that it is hydrophobic, and a dissociation constant value of 8.36, indicating ionization in basic solutions. However, the ionization can be controlled using an acidic mobile phase.…”