Understanding and Exploiting Post-Translational Modifications for Plant Disease Resistance

Gough, Catherine; Sadanandom, Ari

doi:10.3390/biom11081122

Cited by 18 publications

(7 citation statements)

References 349 publications

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“…As shown in Figure 2B, both GFP-C2 proteins could associate with AtJAZ8-RFP and SlJAZ9-RFP, while free GFP did not. In the input, we observed one intense band corresponding to the predicted size of JAZ proteins fused to RFP (around 40 KDa) and a heavier but faint band (approximately 50 KDa) that could correspond to post-translational modifications by ubiquitin or SUMO, previously detected in JAZ proteins (Gough and Sadanandom, 2021). Notably, JAZ proteins co-immunoprecipitated with both C2 proteins showed these two bands with similar intensities.…”

Section: Resultsmentioning

confidence: 54%

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the viral infection in Arabidopsis

Rosas-Díaz

Cana-Quijada

et al. 2022

Preprint

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Jasmonates (JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN (JAZ) proteins are crucial keeping JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system, allowing the activation of the downstream transcription factors and, consequently, the activation of JA-responsive genes. A growing body of evidence has shown that JA signalling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2 proteins from two geminiviruses from the genus Begomovirus, tomato yellow curl Sardinia virus (TYLCSaV) and tomato yellow leaf curl virus (TYLCV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2 proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8 did not lead to altered responses to TYLCV infection; however, knock-down of JAZ8 favours the geminiviral infection in plants. Low levels of JAZ8 likely affect the viral infection specifically since JAZ8-silenced plants do not display developmental phenotypes nor present differences in their interaction with the viral insect vector. Our results show that JAZ8 interacts with geminiviral C2 proteins and exerts an anti-geminiviral effect.

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Section: Resultsmentioning

confidence: 54%

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the viral infection in Arabidopsis

Rosas-Díaz

Cana-Quijada

et al. 2022

Preprint

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show abstract

“…As shown in Figure 2B, GFP‐C2‐TY associate with AtJAZ8‐RFP and SlJAZ9‐RFP, while free GFP does not. In the input, we observed one intense band corresponding to the predicted size of JAZ proteins fused to RFP (around 40 kDa) and a heavier but faint band (approximately 50 kDa) that could correspond to post‐translational modifications by ubiquitin or SUMO, previously detected in JAZ proteins (Gough and Sadanandom, 2021). Notably, JAZ proteins co‐immunoprecipitated with C2‐TY showed these two bands with similar intensities.…”

Section: Resultsmentioning

confidence: 58%

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis

Rosas-Díaz

Cana-Quijada

et al. 2023

JIPB

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Jasmonates (JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN (JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system, allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2 proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus (TYLCV) and tomato yellow curl Sardinia virus (TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2 proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8 did not lead to altered responses to TYLCV infection in Arabidopsis; however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically, since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect.

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“…At the current stage, with the rapid development of modern technologies, such as next generation sequencing, multi-omics analysis, the gene-editing system clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9), genome-wide association studies (GWAS), and others, the exploration and dissection of AP2/ERFs regulatory network will broaden our understanding of their roles in plant disease resistance. It is worth to mention that, in addition to transcriptional regulation, post-translational modification (PTM) of proteins are also tightly associated with the regulation of plant immunity (Gough and Sadanandom 2021 ; Withers and Dong 2017 ; Yin, et al 2019 ). PTMs are highly specific alterations of protein structures, and most of them are rapid and reversible in plant cells (Jensen 2004 ).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Plant disease resistance outputs regulated by AP2/ERF transcription factor family

Ma,

Sun,

et al. 2024

Stress Biology

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Plants have evolved a complex and elaborate signaling network to respond appropriately to the pathogen invasion by regulating expression of defensive genes through certain transcription factors. The APETALA2/ethylene response factor (AP2/ERF) family members have been determined as key regulators in growth, development, and stress responses in plants. Moreover, a growing body of evidence has demonstrated the critical roles of AP2/ERFs in plant disease resistance. In this review, we describe recent advances for the function of AP2/ERFs in defense responses against microbial pathogens. We summarize that AP2/ERFs are involved in plant disease resistance by acting downstream of mitogen activated protein kinase (MAPK) cascades, and regulating expression of genes associated with hormonal signaling pathways, biosynthesis of secondary metabolites, and formation of physical barriers in an MAPK-dependent or -independent manner. The present review provides a multidimensional perspective on the functions of AP2/ERFs in plant disease resistance, which will facilitate the understanding and future investigation on the roles of AP2/ERFs in plant immunity.

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Understanding and Exploiting Post-Translational Modifications for Plant Disease Resistance

Cited by 18 publications

References 349 publications

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the viral infection in Arabidopsis

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the viral infection in Arabidopsis

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis

Plant disease resistance outputs regulated by AP2/ERF transcription factor family

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