Understanding a key gene for thermosensitive genic male sterility in rice

Yang, Fanyi; Zhang, Qifa

doi:10.1007/s11427-014-4763-6

Cited by 4 publications

(3 citation statements)

References 4 publications

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“…Aberrant sensitivity to temperature fluctuation is a hallmark of mutants with defects in ribosome biogenesis in E. coli (Guthrie et al, 1969;Dammel and Noller, 1993;Jones et al, 1996;Al Refaii and Alix, 2009;Mayerle and Woodson, 2013), yeast (Warner and Udem, 1972;Tollervey et al, 1993;Teyssier et al, 2003;Wan et al, 2015), and Arabidopsis (Ohbayashi et al, 2011;Huang et al, 2016;Liu et al, 2016). In rice, temperature fluctuations such as heat and chilling stresses adversely affect the vegetative and reproductive stages (Zhou et al, 2012(Zhou et al, , 2014Fan and Zhang, 2014), which eventually affect yields (Cruz et al, 2013;Ray et al, 2015). Likewise, dysfunction of the ribosome biogenesis factor TOGR1 affected pre-rRNA processing, which resulted in severe developmental defects and hypersensitivity to heat stress in rice.…”

Section: Discussionmentioning

confidence: 99%

“…Also, constitutive expression of TOGR1 enhanced the tolerance of rice to heat stress (Wang et al, 2016). Therefore, an understanding of ribosome biogenesis and its response to ambient temperature in rice can benefit basic scientific research and facilitate efforts to improve thermo-tolerance (Chen et al, 2009;Song et al, 2012aSong et al, , 2012bZhou et al, 2014;Li et al, 2015;Shen et al, 2015;Wang et al, 2016;Yu et al, 2018) and chilling tolerance (Fan and Zhang, 2014;Lu et al, 2014;Ma et al, 2015;Li and Lin, 2016;Zhang et al, 2017 b) in agricultural applications.…”

Section: Discussionmentioning

confidence: 99%

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Ribosomal RNA Biogenesis and Its Response to Chilling Stress in Oryza sativa

et al. 2018

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Ribosome biogenesis is crucial for plant growth and environmental acclimation. Processing of ribosomal RNAs (rRNAs) is an essential step in ribosome biogenesis and begins with transcription of the rDNA. The resulting precursor-rRNA (pre-rRNA) transcript undergoes systematic processing, where multiple endonucleolytic and exonucleolytic cleavages remove the external and internal transcribed spacers (ETS and ITS). The processing sites and pathways for pre-rRNA processing have been deciphered in and, to some extent, in, mammalian cells, and Arabidopsis (). However, the processing sites and pathways remain largely unknown in crops, particularly in monocots such as rice (), one of the most important food resources in the world. Here, we identified the rRNA precursors produced during rRNA biogenesis and the critical endonucleolytic cleavage sites in the transcribed spacer regions of pre-rRNAs in rice. We further found that two pre-rRNA processing pathways, distinguished by the order of 5' ETS removal and ITS1 cleavage, coexist in vivo. Moreover, exposing rice to chilling stress resulted in the inhibition of rRNA biogenesis mainly at the pre-rRNA processing level, suggesting that these energy-intensive processes may be reduced to increase acclimation and survival at lower temperatures. Overall, our study identified the pre-rRNA processing pathway in rice and showed that ribosome biogenesis is quickly inhibited by low temperatures, which may shed light on the link between ribosome biogenesis and environmental acclimation in crop plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ribosomal RNA Biogenesis and Its Response to Chilling Stress in Oryza sativa

et al. 2018

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“…Discovery and successful application of two-line male sterile rice germplasm resources play important roles in ensuring food safety in China (Dong et al, 2000;Fan and Zhang, 2014). Compared with three-line hybrid rice, two-line hybrid rice is not limited by restore gene(s) and maintainer gene(s), which widely broadens the range of germplasm resources (Nas et al, 2005;Peng et al, 2010;Qi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Development of SNP-based dCAPS markers for identifying male sterile gene tms5 in two-line hybrid rice

Song

Qian

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Molecular markers can increase both the efficiency and speed of breeding programs. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. In this study, we used newly derived cleaved amplified polymorphic sequence markers to detect the functional mutations of tms5, which is a male sterile gene that is widely used in rice production in China. In addition, restriction cutting sites were designed to specifically digest amplicons of tms5 but not wild type (TMS5), in order to avoid the risk of false positive results. By optimizing the condition of the polymerase chain reaction amplifications and restriction enzyme digestions, the newly designed markers could accurately distinguish between tms5 and TMS5. These markers can be applied in marker-assisted selection for breeding novel thermo-sensitive genic male sterile (TGMS) lines, as well as to rapidly identify the TGMS hybrid seed purity.

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Genetic and molecular characterization of photoperiod and thermo-sensitive male sterility in rice

Yang

Zhang

2017

Plant Reprod

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A review on photoperiod and temperature-sensitive genic male sterility in rice. Male sterility in plants, facilitating the development of hybrid crops, has made great contribution to crop productivity worldwide. Environment-sensitive genic male sterility (EGMS), including photoperiod-sensitive genic male sterility (PGMS) and temperature-sensitive genic male sterility (TGMS), has provided a special class of germplasms for the breeding of "two-line" hybrids in several crops. In rice, the finding of the PGMS NK58S mutant in 1973 started the journey of research and breeding of two-line hybrids. Genetic and molecular characterization of these germplasms demonstrated diverse genes and molecular mechanisms of male sterility regulation. Two loci identified from NK58S, PMS1 and PMS3, both encode long noncoding RNAs. A major TGMS locus, TMS5, found in the TGMS line Annong S-1, encodes an RNase Z. A reverse PGMS mutant carbon starved anther encodes an R2R3 MYB transcription factor. Breeding efforts in the last three decades have resulted in hundreds of EGMS lines and two-line hybrids released to rice production, which have greatly elevated the yield potential and grain quality of rice varieties. The enhanced molecular understanding will offer new strategies for the development of EGMS lines thus further improving two-line hybrid breeding of rice as well as other crops.

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Understanding a key gene for thermosensitive genic male sterility in rice

Cited by 4 publications

References 4 publications

Ribosomal RNA Biogenesis and Its Response to Chilling Stress in Oryza sativa

Ribosomal RNA Biogenesis and Its Response to Chilling Stress in Oryza sativa

Development of SNP-based dCAPS markers for identifying male sterile gene tms5 in two-line hybrid rice

Genetic and molecular characterization of photoperiod and thermo-sensitive male sterility in rice

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