Underlying Promotion Mechanism of High Concentration of Silver Nanoparticles on Anammox Process

Peng, Meng-Wen; Yu, Xiuling; Guan, Yong; Liu, Peng; Yan, Peng; Fang, Fang; Guo, Jinsong; Chen, You‐Peng

doi:10.1021/acsnano.9b08263

Cited by 65 publications

(35 citation statements)

References 74 publications

(128 reference statements)

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“…Such beneficial effects of ENMs have been reported in enzyme production, wastewater treatment, and environmental remediation. Surprisingly, Ag NPs which typically exert antibacterial effects, were found to have a stimulatory effect on the anaerobic ammonium oxidizing (anammox) process in bacterial granules ( Peng et al, 2019 ). Ag NPs acted by increasing the porosity of the anammox granules which enhanced the diffusion of nutrients and iron ions into the granules which, in turn, upregulated anammox-related enzyme expression as identified using proteomics analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Second, risk assessment of ENM exposures to environmental and beneficial bacteria may be of interest ( Holden et al, 2013 , 2014b ). Lastly, as an emerging trend, ENMs can stimulate beneficial bacterial functions ( Mu et al, 2019 ; Peng et al, 2019 ; Ren et al, 2019b ; Yan et al, 2019 ). Successful accomplishment of these aims requires detailed knowledge of the underlying mechanisms of ENM-bacterial interactions.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Mortimer

Wang

Holden

2021

Front. Bioeng. Biotechnol.

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Nanotechnology is employed across a wide range of antibacterial applications in clinical settings, food, pharmaceutical and textile industries, water treatment and consumer goods. Depending on type and concentration, engineered nanomaterials (ENMs) can also benefit bacteria in myriad contexts including within the human body, in biotechnology, environmental bioremediation, wastewater treatment, and agriculture. However, to realize the full potential of nanotechnology across broad applications, it is necessary to understand conditions and mechanisms of detrimental or beneficial effects of ENMs to bacteria. To study ENM effects, bacterial population growth or viability are commonly assessed. However, such endpoints alone may be insufficiently sensitive to fully probe ENM effects on bacterial physiology. To reveal more thoroughly how bacteria respond to ENMs, molecular-level omics methods such as transcriptomics, proteomics, and metabolomics are required. Because omics methods are increasingly utilized, a body of literature exists from which to synthesize state-of-the-art knowledge. Here we review relevant literature regarding ENM impacts on bacterial cellular pathways obtained by transcriptomic, proteomic, and metabolomic analyses across three growth and viability effect levels: inhibitory, sub-inhibitory or stimulatory. As indicated by our analysis, a wider range of pathways are affected in bacteria at sub-inhibitory vs. inhibitory ENM effect levels, underscoring the importance of ENM exposure concentration in elucidating ENM mechanisms of action and interpreting omics results. In addition, challenges and future research directions of applying omics approaches in studying bacterial-ENM interactions are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Mortimer

Wang

Holden

2021

Front. Bioeng. Biotechnol.

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“…The anammox granules were cultivated in a 2 l expanded granular sludge blanket (EGSB) reactor (Xing et al, 2017;Peng et al, 2019). The reactor has been running steadily for five years and the mineral medium (per litre demineralized water) consists of NH 4 Cl, 764 mg (200 mg l À1 NH 4 N); NaNO 2 , 985 mg (200 mg l À1 NO 2 N); KHCO 3 , 2.133 g; KH 2 PO 4 , 25 mg; MgSO 4 Á0.7H 2 O, 200 mg; CaCl 2 Á0.2H 2 O, 200 mg; FeSO 4 , 0.03 mM; and 1 ml of trace element solution I and II as described by van de Graaf et al (1996).…”

Section: Cultivation and Purification Of The Anammox Granulesmentioning

confidence: 99%

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

Peng

Guan

Liu

et al. 2020

J Synchrotron Radiat

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Anaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to its function; however, research on the ultrastructure of intact anammox bacteria is lacking. In this study, in situ three-dimensional nondestructive ultrastructure imaging of a whole anammox cell was performed using synchrotron soft X-ray tomography (SXT) and the total variation-based simultaneous algebraic reconstruction technique (TV-SART). Statistical and quantitative analyses of the intact anammox bacteria were performed. High soft X-ray absorption composition inside anammoxosome was detected and verified to be relevant to iron-binding protein. On this basis, the shape adaptation of the anammox bacteria response to iron was explored.

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“…The total proteins of the E. coli were evaluated using SDS-PAGE. Proteins were extracted and digested according to a previously published method [42,43]. Detailed information is given in the supplementary information.…”

Section: Lc-ms/ms Proteomic Analysismentioning

confidence: 99%

The role of an encapsulin nanocompartment in resisting silver ion stress

Xing

Guo

et al. 2020

Preprint

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Background: Compartmentalization can protect cells from the interference of external toxic substances by sequestering toxic products. We hypothesized that proteinaceous nanocompartments may be a feasible candidate material to be added to genetically modified bacteria for the sequestration of toxic environmental products, which would open up a new biological detoxification pathway. Results: Here, we identified a new mechanism by which bacteria resist silver ion stress. We showed that the self-assembling nanocompartments helped a model species ( E. coli ) resist silver ion stress. Transmission electron microscopy and energy dispersive X-ray (TEM-EDX) analysis showed that the nanocompartments combined stably with silver ions in vitro . In addition, when exposed to 30 μM AgNO 3 , the survival rate of genetically modified bacteria (with nanocompartments) was 86%, while it was just 59% in the wild-type bacteria (without nanocompartments). Label-free quantitative proteomics indicated that the nanocompartments enhanced bacterial activity by inducing the up-regulation of protein processing and secondary metabolites, and decreased their intracellular silver ion concentration, both of which contributed to their increased resistance to toxic silver ions. Conclusions: This study on nanocompartments has contributed to a deeper understanding of how bacteria respond to environmental stressors like heavy metal pollutants in water, soil, and sediment. The encapsulin nanocompartment has the potential to be applied in various environments.

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Underlying Promotion Mechanism of High Concentration of Silver Nanoparticles on Anammox Process

Cited by 65 publications

References 74 publications

Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

The role of an encapsulin nanocompartment in resisting silver ion stress

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