Underlying mechanism for the modulation of apoptosis induced by a new benzoindole derivative on HT-29 colon cancer cells

Hajiaghaalipour, Fatemeh; Bagheri, Elham; Faraj, Fadhil Lafta; Abdulla, Mahmood Ameen; Majid, Nazia Abdul

doi:10.1039/c7ra03875e

Cited by 6 publications

(3 citation statements)

References 32 publications

(32 reference statements)

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“…BD FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA) instrument was used for fellow cytometric analysis and quadrant statistics was used to identify early and late apoptotic cells and necrotic cells. 28 …”

Section: Methodsmentioning

confidence: 99%

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation

Bagheri

Hajiaghaalipour

Nyamathulla

et al. 2018

DDDT

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BackgroundBrucea javanica (L.) Merr. is a plant from the genus Brucea, which is used in local traditional medicine to treat various diseases. Recent studies revealed an impressive anticancer efficiency of B. javanica extract in different types of cancer cells.PurposeIn this study, we have investigated the cytotoxic effects of the B. javanica hexane, ethanolic extracts against colon cancer cells. HT29 colon cells were selected as an in vitro cancer model to evaluate the anticancer activity of B. javanica ethanolic extract (BJEE) and the possible mechanisms of action that induced apoptosis.Methods3-(4,5-dimethylthiazol-2-yl)-2, 5,-diphenyltetrazolium bromide (MTT), lactate dehydrogenase, acridine orange/propidium iodide, and annexin-V-fluorescein isothiocyanate assays were performed to determine the antiproliferative and apoptosis validation of BJEE on cancer cells. Measurement of reactive oxygen species (ROS) production, caspase activities, nucleus factor-κB activity, and gene expression experiments was done to investigate the potential mechanisms of action in the apoptotic process.ResultsThe results obtained from this study illustrated the significant antiproliferative effect of BJEE on colorectal cancer cells, with a concentration value that inhibits 50% of the cell growth of 25±3.1 µg/mL after 72 h of treatment. MTT assay demonstrated that the BJEE is selectively toxic to cancer cells, and BJEE induced cell apoptosis via activation of caspase-8 along with modulation of apoptosis-related proteins such as Fas, CD40, tumor necrosis factor-related apoptosis-inducing ligands, and tumor necrosis factor receptors, which confirmed the contribution of extrinsic pathway. Meanwhile, increased ROS production in treated cells subsequently activated caspase-9 production, which triggered the intrinsic pathways. In addition, overexpression of cytochrome-c, Bax, and Bad proteins along with suppression of Bcl-2 illustrated that mitochondrial-dependent pathway also contributed to BJEE-induced cell death. Consistent with the findings from this study, BJEE-induced cancer cell death proceeds via extrinsic and intrinsic mitochondrial-dependent and -independent events.ConclusionFrom the evidence obtained from this study, it is concluded that the BJEE is a promising natural extract to combat colorectal cancer cells (HT29 cells) via induction of apoptosis through activation of extrinsic and intrinsic pathways.

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Section: Methodsmentioning

confidence: 99%

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation

Bagheri

Hajiaghaalipour

Nyamathulla

et al. 2018

DDDT

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“…During the late stage of apoptosis or necrosis, PI penetrates the cells that have lost membrane integrity to allow DNA staining ( Atasever-arslan et al., 2016 ). Four populations of cells are analyzed based on the staining:- unlabeled (viable cells, Annexin V − /PI − ), labeled with Annexin V-FITC only (early apoptotic, Annexin V + /PI − ), stained with PI (necrotic, Annexin V − /PI + ), and bound Annexin V and stained with PI (late apoptotic/necrotic cells, Annexin V + /PI + ) ( Skerman et al., 2011 ; Hajiaghaalipour et al., 2017 ). Following the TMX-MCEs (1:3) synergistic treatment in MCF-7 cells, significant apoptotic activities were observed, especially the late apoptosis after 24 h with TMX- N. oculata -ETH (41.1 %), TMX- Chlorella sp.-CHL (36.8 %), TMX- T. suecica -ETH (36.6 %) and TMX- N. oculata -W (32.8 %).…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity and 1H NMR metabolomics analyses of microalgal extracts for synergistic application with Tamoxifen on breast cancer cells with reduced toxicity against Vero cells

Hussein

Kassim

Maulidiani

et al. 2022

Heliyon

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“…The luminescence of each sample was measured in a plate-reading luminometer (GloMax microplate luminescence reader, Promega Company, USA). 18 …”

Section: Methodsmentioning

confidence: 99%

Ethanolic extract ofBrucea javanicainhibit proliferation of HCT-116 colon cancer cellsviacaspase activation

et al. 2018

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Underlying mechanism for the modulation of apoptosis induced by a new benzoindole derivative on HT-29 colon cancer cells

Abstract: DBID compound induced LDH leakage in HT-29 cells when compared to untreated cells.

Cited by 6 publications

References 32 publications

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation

Cytotoxicity and 1H NMR metabolomics analyses of microalgal extracts for synergistic application with Tamoxifen on breast cancer cells with reduced toxicity against Vero cells

Ethanolic extract ofBrucea javanicainhibit proliferation of HCT-116 colon cancer cellsviacaspase activation

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Underlying mechanism for the modulation of apoptosis induced by a new benzoindole derivative on HT-29 colon cancer cells

Abstract: DBID compound induced LDH leakage in HT-29 cells when compared to untreated cells.

Cited by 6 publications

References 32 publications

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-&kappa;B translocation

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-&kappa;B translocation

Cytotoxicity and 1H NMR metabolomics analyses of microalgal extracts for synergistic application with Tamoxifen on breast cancer cells with reduced toxicity against Vero cells

Ethanolic extract ofBrucea javanicainhibit proliferation of HCT-116 colon cancer cellsviacaspase activation

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The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation