Underlying formation mechanisms of ultrasound-assisted brined porcine meat: The role of physicochemical modification, myofiber fragmentation and histological organization

Effect of ultrasound combined with pineapple protease treatment on the tenderness of dried shrimp

“…Bromophenol blue (BPB) is a commonly employed tool to monitor conformational alterations in MPs and to assess the exposure of hydrophobic sites 32 . In general, partial swelling of animal proteins leads to the exposure of hydrophobic amino acid residues that are typically concealed within their structures, thereby leading to an increase in surface hydrophobicity 60 . Figure 2(C) illustrates a significant increase ( P < 0.05) in the surface hydrophobicity of the treatment group.…”

“…32 In general, partial swelling of animal proteins leads to the exposure of hydrophobic amino acid residues that are typically concealed within their structures, thereby leading to an increase in surface hydrophobicity. 60 Figure 2(C) illustrates a significant increase (P < 0.05) in the surface hydrophobicity of the treatment group. This phenomenon can be attributed to the partial denaturation of protein structures induced by enzymes and ultrasound.…”

Effect of packaging thickness and muscle type on ultrasound-assisted beef quality

“…Therefore, both the epimysium and the perimysium have greater exposure to ultrasound because they are located in the outermost part of the muscle fibers and surround the entire muscle and fascicles, respectively. Destruction of the perimysial structure and, to a lesser extent, the production of microcracks in the intermyofibrillar spaces have been documented [48] in ultrasound-assisted marinated pork (20 kHz, 315 W, 1 h). With times greater than 10 min of HIU (40 kHz, 1500 W), shrinkage of the fibers, rupture of the endomysium and decrease of the perimysium have been reported in bovine Semitendinosus [29] .…”

“…Similar to the present study, these researchers reported water losses and an increase in meat tenderness due to the effect of HIU. On the other hand, ultrasound arranges the myofibrils more orderly and retains finer connective tissue between the fibers [48] . This implies a higher ordering in the myofibrillar structure in G. medius and B. femoris when 70 µm package film was used ( Fig.…”

“…The deformation of the myofibril bundles by increasing the spaces between them has been attributed to ultrasonic cavitation, however the use of brine produces an additional microinjection effect dependent on the concentration of salts during marinating [48] , [49] . Similar to G. medius findings, the interfibrillar spaces in B. femoris were significantly increased with 40 min HIU ( Table 2 , Fig.…”