Underlying beneficial effects of Rhubarb on constipation-induced inflammation, disorder of gut microbiome and metabolism

Gao, Han; He, Chengwei; Hua, Rongxuan; Liang, Chen; Wang, Boya; Du, Yi-Xuan; Xin, Shuzi; Guo, Yuexin; Gao, Lei; Zhang, Lucia; Shang, Hongcai; Xu, Jin

doi:10.3389/fphar.2022.1048134

Cited by 8 publications

(5 citation statements)

References 60 publications

(70 reference statements)

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“…Patients with constipation often have impaired intestinal barrier function and varying degrees of intestinal inflammation. Previous studies have shown that a reduction in constipation symptoms is accompanied by the reduction of inflammation ( Liu et al, 2020 ; Gao H. et al, 2022 ; Shatri et al, 2023 ). M. oleifera leaf aqueous extract was able to alleviate high-fat diet-induced intestinal inflammation ( Mohamed Husien et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice

Gao,

Yang,

et al. 2023

Front. Microbiol.

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Moringa oleifera Lam. leaf is not only a new food resource in China, but also a traditional medicinal plant. It is commonly used in the folk to alleviate constipation, but its laxative mechanism is not fully understood. Hence we investigated it in loperamide-induced functional constipation (FC) mice. The results showed that MOAE significantly regulated not only gastrointestinal hormones and neurotransmitters in serum but also important gastrointestinal motility factors in the enteric nervous system (ENS)-interstitial cells of Cajal (ICCs)-smooth muscle cell (SMC) network. Meanwhile, MOAE attenuated intestinal inflammation, increased cecal short-chain fatty acid levels and colonic antimicrobial peptide expression, and improved the impaired intestinal barrier function in loperamide-induced FC mice. In addition, MOAE also increased fecal water content by inhibiting the mRNA expression of colonic aquaporins (Aqp3 and Aqp4) in FC mice. Interestingly and importantly, MOAE affected the intestinal microbiota by inhibiting some key “constipation-causing” microbiota, such as Bacteroidaceae, Clostridiaceae, Bacteroides, and Ruminococcus, and promoting the growth of other important “constipation-curing” microbiota, such as Butyricoccus, Tyzzerella, and Desulfovibrio. These important taxa are significantly associated with a variety of indicators of constipation. These findings suggest that MOAE can promote defecation through its rich chemical composition to modulate the ENS-ICCs-SMCs network and the gut microecosystem.

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Section: Discussionmentioning

confidence: 99%

Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice

Gao,

Yang,

et al. 2023

Front. Microbiol.

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“…A targeted investigation of IL-17A in individuals with moderate to severe asthma and high reversibility may yield signi cant clinical advantages [51]. In constipated mice, there is evidence of heightened muscle thickness, elevated cytokine levels such as IL-17 and IL-23, and decreased levels of IL-22 [52].…”

Section: Go and Kegg Pathway Analysismentioning

confidence: 99%

A Joint Action Mechanism of “Trichosanthis Fructus-Almond” Herb Pair in the Treatment of Asthma with Functional Constipation Based on the Network Pharmacology and Molecular Docking

Dai,

Chen,

et al. 2024

Preprint

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Background：The herb combination of “Trichosanthis Fructus-Almond” herb pair is frequently employed in the treatment of respiratory ailments such as asthma and chronic obstructive pulmonary disease, and also offer some relief for constipation. However, its bioactive ingredients and mechanisms of action remain unclear. Objective: This study aimed to explore the latent mechanism of action of “Trichosanthis Fructus-Almond” herb pair in treating asthma combined with functional constipation based on network pharmacology and molecular docking. Methods: Firstly, we searched the TCMSP database to obtain the potential active ingredients of the herb pair “Trichosanthis Fructus-Amond” and their associated molecular targets. Databases such as Genecards, OMIM, TTD, and DrugBankwere enrolled to identify the targets associated with asthma and functional constipation. Then, the common targets of the “Trichosanthis Fructus-Almond” herb pair for treating asthma and functional constipation were determined by intersecting the predicted active ingredient targets with disease-related targets. Simultaneously, we visited String 11.0 to analyze the relationship within common drug-disease targets to obtain PPI network relationship. The PPI network diagram was generated using Cytoscape 3.7.2 to examine the vital targets. Furthermore, Metascape platform was utilized for GO function and KEGG pathway enrichment analysis among the shared targets. A drug component-target-pathway network diagram was lighten using Cytoscape 3.7.2. After analyzing the potential core components, molecular docking was, finally, employed to validate the core components and key targets using Autodock vina. Results: A total of 28 active components of Trichosanthis Fructus-Almond were obtained, and 27 potential targets (common targets) of “Trichosanthis Fructus-Almond” herb pair for the treatment of asthma combined with functional constipation were identified, involving 373 biological processes, 35 cellular components, 58 molecular functions, and 66 signaling pathways. primary active components were subsequently examined, like Ziziphin_qt, Stigmasterol, l-SPD, Glabridin, Estrone, Machiline, Licochalcone B, Diisooctyl succinate, Phaseol, (+)-catechin. Besides, key target proteins, such as NR3C1, PPARG, PTGS2, MAPK14, ESR1, and various pathways, including Neurodegeneration-Multiple Diseases, Chemical Carcinogenesis-Receptor Activation, Pathways of Cancer, Endocrine Resistance, NOD-like Receptors, IL-17, et, al. were uncovered. There is strong binding activity in molecular docking among key targets and potential core components. Conclusions: The current research showed that “Trichosanthis Fructus-Almond” herb pairhas various components, targets, and pathways for treating functional constipation in asthma, which may insight the further studies on its mechanism of action. This could be a crucial mechanism for managing respiratory and gastrointestinal conditions.

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“…Studies have also indicated that constipated humans or mice have a distinct gut microbiome signature and mild inflammation compared to healthy individuals. 14 Patients with constipation show lower relative abundances of Bacteroidetes (Bacteroides and Prevotella), Bifidobacteria, Lactobacilli, and Roseburia within gut microbiome than healthy one. 1 Although there is insufficient evidence to confirm the direct amelioration of phenolics on constipation, we observed that lychee pulp phenolics restructure the gut microbiota and promote biosynthesis of SCFAs that alleviate constipation and gut inflammation partly by activating free fatty acid receptors.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Meanwhile, dietary fiber serves as a food matrix, which may affect the bioaccessibility and bioavailability of bound phenolics in the digestive tract. Studies have also indicated that constipated humans or mice have a distinct gut microbiome signature and mild inflammation compared to healthy individuals . Patients with constipation show lower relative abundances of Bacteroidetes (Bacteroides and Prevotella), Bifidobacteria, Lactobacilli, and Roseburia within gut microbiome than healthy one .…”

Section: Introductionmentioning

confidence: 99%

Lychee Pulp-Derived Dietary Fiber-Bound Phenolic Complex Upregulates the SCFAs-GPRs-ENS Pathway and Aquaporins in Loperamide-Induced Constipated Mice by Reshaping Gut Microbiome

Dong,

Xu,

Huang

et al. 2023

J. Agric. Food Chem.

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This study aimed to investigate the effects of the lychee pulp-derived dietary fiber-bound phenolic complex (DF-BPC) on a murine model of loperamide-induced constipation and its molecular mechanism associated with gut microbiota modification. DF-BPC supplementation mitigated loperamide-induced dyschezia, intestinal hypomotility, and colonic impairment, as evidenced by the increased gastro-intestinal transit rate and mucus cell counts. By comparison, short-chain fatty acids (SCFAs) contents and relative abundances of associated genera (Butyricimonas, Clostridium, and Lactobacillus) were effectively upregulated following DF-BPC supplementation. Notably, DF-BPC significantly enhanced expressions of G protein-coupled receptor (GPR) 41 and 43, reaching 1.43-and 1.62-fold increase, respectively. Neurotransmitter secretions were simultaneously altered in DF-BPCtreated mice, suggesting upregulation of the SCFAs-GPRs-enteric nervous system pathway. The overexpression of aquaporins (AQP3, 8, and 9) was stimulated partly through GPRs activation. Mild inflammation associated with constipation was inhibited by suppressing LBP-TLR4-NF-κB signaling translocation. These findings suggest that DF-BPC from lychee pulp has the potential to alleviate constipation in mice through modifying the gut microbiome.

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Underlying beneficial effects of Rhubarb on constipation-induced inflammation, disorder of gut microbiome and metabolism

Cited by 8 publications

References 60 publications

Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice

Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice

A Joint Action Mechanism of “Trichosanthis Fructus-Almond” Herb Pair in the Treatment of Asthma with Functional Constipation Based on the Network Pharmacology and Molecular Docking

Lychee Pulp-Derived Dietary Fiber-Bound Phenolic Complex Upregulates the SCFAs-GPRs-ENS Pathway and Aquaporins in Loperamide-Induced Constipated Mice by Reshaping Gut Microbiome

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