Under the (Light) Sheet after the iDISCO+

A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice

“…Unfortunately, the cervix appeared to be open in its proximal part toward the vagina, and the absence of mucus in this same region suggested that part of the CMP was probably lost during the different steps of the sample preparation. In addition, the iDISCO protocol produces significant shrinkage of the tissue by up to 50% of its volume ( Velíšek, 2016 ), which likely reflects loss of its watery mucus content and highlights the need for improving the protocol to ensure better preservation of the tissue structure and its mucus gel.…”