Under the African sun; a description of native races in Uganda, sporting adventures and other experiences, by W. J. Ansorge.

Ansorge, William John; Hartert, Ernst

doi:10.5962/bhl.title.27723

Cited by 38 publications

(38 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were dissolved in O'Farrell sample buffer for electrophoresis as already described. The two-dimensional polyacrylamide gel was then silver stained according to the procedure of Ansorge (1982). Drosophila (Graziosiet al, 1980) and sea urchin (Roccheri et al, 1981).…”

Section: Ec Cellsmentioning

confidence: 99%

Spontaneous high expression of heat-shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo.

1983

View full text Add to dashboard Cite

When submitted to a heat-shock, mouse embryonal carcinoma (EC) and fibroblast cells show very different behavior. AN the EC cells so far analyzed express very high levels of several heat-shock proteins (HSP) in the absence of stress and independent of their origin and culture conditions. In such cells, the 89-kd, 70-kd and 59-kd HSP are the most prominent proteins after actin. In addition, the 89-kd and 59-kd HSP are not stimulated by an arsenite shock in contrast to what is observed with fibroblasts or cells of the parietal yolk sac type. Arsenite induces the synthesis of a 105-kd polypeptide in fibroblasts but not in EC cells. In vitro differentiation of F9 cells induced by retinoic acid and dibutyryl cAMP is accompanied by a decrease in the spontaneous relative abundance of HSP and restores the arsenite-induced synthesis of the 105-kd polypeptide. EC cells are usually believed to be similar to inner cell mass cells of mouse blastocyst. Furthermore, data in the literature together with our own results suggest that the same three HSP are also spontaneously expressed in high amounts in the early mouse embryo.

show abstract

Section: Ec Cellsmentioning

confidence: 99%

Spontaneous high expression of heat-shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo.

1983

View full text Add to dashboard Cite

show abstract

“…Peptide mapping and silver staining Limited proteolysis of gel spots performed with Staphylococcus aureus V8 protease and peptide analysis were carried out according to Cleveland et al (1977). The gels were silver stained according to Ansorge (1983).…”

Section: Tubulin Purificationmentioning

confidence: 99%

Two specific markers for neural differentiation of embryonal carcinoma cells.

1983

View full text Add to dashboard Cite

Two multipotential embryonal carcinoma (EC) cell lines, 1003 and 1009, can be induced to form preferentially neural derivatives in vitro. Synthesis of specific proteins during neural differentiation was followed by two-dimensional gel electrophoresis. The comparison of protein patterns obtained with neural and non-neural derivatives of these EC cell lines indicates that two changes are specific for the neural pathway: (i) the appearance of a new (3-tubulin isoform and (ii) the accumulation of the brain isozyme of creatine phosphokinase already present in small amounts in EC stem cells. These changes were found to take place early in the course of differentiation and to occur even when neurite outgrowth was prevented.

show abstract

“…2. The glass assembly (3,4) was placed on top of the bottom gasket (5), and a rubber gasket with formed openings for the cathode, anode, and load region was placed on top (2). An integrated water channel ran under the glass assembly and water was pumped through this channel to maintain the glass at a constant and uniform temperature.…”

Section: Cassette Assemblymentioning

confidence: 99%

A transmission imaging spectrograph and microfabricated channel system for DNA analysis

et al. 2000

View full text Add to dashboard Cite

In this paper we present the development of a DNA analysis system using a microfabricated channel device and a novel transmission imaging spectrograph which can be efficiently incorporated into a high throughput genomics facility for both sizing and sequencing of DNA fragments. The device contains 48 channels etched on a glass substrate. The channels are sealed with a flat glass plate which also provides a series of apertures for sample loading and contact with buffer reservoirs. Samples can be easily loaded in volumes up to 640 nL without band broadening because of an efficient electrokinetic stacking at the electrophoresis channel entrance. The system uses a dual laser excitation source and a highly sensitive charge‐coupled device (CCD) detector allowing for simultaneous detection of many fluorescent dyes. The sieving matrices for the separation of single‐stranded DNA fragments are polymerized in situ in denaturing buffer systems. Examples of separation of single‐stranded DNA fragments up to 500 bases in length are shown, including accurate sizing of GeneCalling® fragments, and sequencing samples prepared with a reduced amount of dye terminators. An increase in sample throughput has been achieved by color multiplexing.

show abstract

Under the African sun; a description of native races in Uganda, sporting adventures and other experiences, by W. J. Ansorge.

Cited by 38 publications

References 0 publications

Spontaneous high expression of heat-shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo.

Spontaneous high expression of heat-shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo.

Two specific markers for neural differentiation of embryonal carcinoma cells.

A transmission imaging spectrograph and microfabricated channel system for DNA analysis

Contact Info

Product

Resources

About