Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

Godon, Christian; Mercier, Claire; Wang, Xiaoyue; David, Pascale; Richaud, Pierre; Nussaume, Laurent; Liu, Dong; Desnos, Thierry

doi:10.1111/tpj.14374

Cited by 78 publications

(83 citation statements)

References 52 publications

(114 reference statements)

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“…Growth arrest requires accumulation of transcription factor STOP1 in the nucleus. Under P-restriction, it has been proposed that Fe stimulates the accumulation of STOP1 in root nuclei which, in turn, activates the transcription of malate transporter gene ALMT1 (Godon et al, 2019). The use of other NO donors, and the measurement of NO levels in each system, will confirm the effect of NO on primary root morphology under P scarcity.…”

Section: Primary and Lateral Rootsmentioning

confidence: 98%

An Update on Nitric Oxide Production and Role Under Phosphorus Scarcity in Plants

et al. 2020

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Section: Primary and Lateral Rootsmentioning

confidence: 98%

An Update on Nitric Oxide Production and Role Under Phosphorus Scarcity in Plants

et al. 2020

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“…Earliest evidence suggested that, because the transcription levels of STOP1 do not significantly change in response to low pH or Al exposure, STOP1 was post-transcriptionally activated ( Sawaki et al., 2009 ). A recent report on STOP1 regulation corroborated that the transcription factor is regulated at the posttranslational level via protein accumulation/stabilization under low Pi and low pH conditions when Fe and Al are present in the medium ( Godon et al., 2019 ). Furthermore, it was demonstrated that STOP1 abundance is regulated by the ubiquitin-proteasome-mediated degradation pathway via a member of the F-box E3-type ubiquitin ligase protein family, REGULATION OF ALMT1 EXPRESSION (RAE1).…”

Section: Introductionmentioning

confidence: 81%

Dissection of Root Transcriptional Responses to Low pH, Aluminum Toxicity and Iron Excess Under Pi-Limiting Conditions in Arabidopsis Wild-Type and stop1 Seedlings

2020

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Acidic soils constrain plant growth and development in natural and agricultural ecosystems because of the combination of multiple stress factors including high levels of Fe 3+ , toxic levels of Al 3+ , low phosphate (Pi) availability and proton rhizotoxicity. The transcription factor SENSITIVE TO PROTON RHIZOTOXICITY (STOP1) has been reported to underlie root adaptation to low pH, Al 3+ toxicity and low Pi availability by activating the expression of genes involved in organic acid exudation, regulation of pH homeostasis, Al 3+ detoxification and root architecture remodeling in Arabidopsis thaliana . However, the mechanisms by which STOP1 integrates these environmental signals to trigger adaptive responses to variable conditions in acidic soils remain to be unraveled. It is unknown whether STOP1 activates the expression of a single set of genes that enables root adaptation to acidic soils or multiple gene sets depending on the combination of different types of stress present in acidic soils. Previous transcriptomic studies of stop1 mutants and wild-type plants analyzed the effect of individual types of stress prevalent in acidic soils. An integrative study of the transcriptional regulation pathways that are activated by STOP1 under the combination of major stresses common in acidic soils is lacking. Using RNA-seq, we performed a transcriptional dissection of wild-type and stop1 root responses, individually or in combination, to toxic levels of Al 3+ , low Pi availability, low pH and Fe excess. We show that the level of STOP1 is post-transcriptionally and coordinately upregulated in the roots of seedlings exposed to single or combined stress factors. The accumulation of STOP1 correlates with the transcriptional activation of stress-specific and common gene sets that are activated in the roots of wild-type seedlings but not in stop1 . Our data indicate that perception of low Pi availability, low pH, Fe excess and Al toxicity converges at two levels via STOP1 signaling: post-translationally through the regulation of STOP1 turnover, and transcriptionally, via the activation of STOP1-dependent gene expression that enables the root to better adapt to abiotic stress factors present in acidic soils.

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“…Moreover, PGIP1 and PGIP2, which were downregulated by STOP2 and associated with cell wall stabilization at low pH [29], were activated being expressed in GsMAS1 transgenic lines under aluminum stress ( Figure 6). As a negative regulator of ALMT1, WRKY46 regulated aluminum-induced malate secretion in Arabidopsis [23], whereas ALS3 protected the growing root from Al toxicity by redistributing accumulated Al away from sensitive tissues [96]. The RNA transcripts of WRKY46 and ALS3 both in wild type and GsMAS1 transgenic lines under aluminum stress were less than those in the treatment of the control (Figure 6).…”

Section: Discussionmentioning

confidence: 98%

GsMAS1 Encoding a MADS-box Transcription Factor Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

Zhang

Yang

et al. 2020

IJMS

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The MADS-box transcription factors (TFs) are essential in regulating plant growth and development, and conferring abiotic and metal stress resistance. This study aims to investigate GsMAS1 function in conferring tolerance to aluminum stress in Arabidopsis. The GsMAS1 from the wild soybean BW69 line encodes a MADS-box transcription factor in Glycine soja by bioinformatics analysis. The putative GsMAS1 protein was localized in the nucleus. The GsMAS1 gene was rich in soybean roots presenting a constitutive expression pattern and induced by aluminum stress with a concentration-time specific pattern. The analysis of phenotypic observation demonstrated that overexpression of GsMAS1 enhanced the tolerance of Arabidopsis plants to aluminum (Al) stress with larger values of relative root length and higher proline accumulation compared to those of wild type at the AlCl3 treatments. The genes and/or pathways regulated by GsMAS1 were further investigated under Al stress by qRT-PCR. The results indicated that six genes resistant to Al stress were upregulated, whereas AtALMT1 and STOP2 were significantly activated by Al stress and GsMAS1 overexpression. After treatment of 50 μM AlCl3, the RNA abundance of AtALMT1 and STOP2 went up to 17-fold and 37-fold than those in wild type, respectively. Whereas the RNA transcripts of AtALMT1 and STOP2 were much higher than those in wild type with over 82% and 67% of relative expression in GsMAS1 transgenic plants, respectively. In short, the results suggest that GsMAS1 may increase resistance to Al toxicity through certain pathways related to Al stress in Arabidopsis.

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Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

Cited by 78 publications

References 52 publications

An Update on Nitric Oxide Production and Role Under Phosphorus Scarcity in Plants

An Update on Nitric Oxide Production and Role Under Phosphorus Scarcity in Plants

Dissection of Root Transcriptional Responses to Low pH, Aluminum Toxicity and Iron Excess Under Pi-Limiting Conditions in Arabidopsis Wild-Type and stop1 Seedlings

GsMAS1 Encoding a MADS-box Transcription Factor Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

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