Under-expression of the Auxin Response Factor<i>Sl-ARF4</i>improves post-harvest behavior of tomato fruits

Sagar, Maha; Chervin, Christian; Roustan, Jean‐Paul; Bouzayen, Mondher; Zouine, Mohamed

doi:10.4161/psb.25647

Cited by 30 publications

(12 citation statements)

References 18 publications

(25 reference statements)

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“…Recent studies have also demonstrated that loss or gain of function of several auxin response genes, such as SlARF4 , SlARF2a , SlIAA17 and SlIAA27 , leads to conspicuous changes in fruit pigment accumulation, sugar content, starch accumulation, phenylpropanoids component, organic acids contents and other fruit quality attributes ( Bassa et al, 2013 ; Sagar et al, 2013a , b ; Hao et al, 2015 ; Su et al, 2015 ). By using a transcriptome analysis approach Li et al (2016) have suggested that exogenous auxin retards tomato ripening process and interferes on the normal expression patterns of many genes involved in metabolic pathways.…”

Section: The Metabolic Behavior Of Organic Acids During Fruit Developmentioning

confidence: 99%

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

et al. 2018

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The pivotal role of phytohormones during fruit development and ripening is considered established knowledge in plant biology. Perhaps less well-known is the growing body of evidence suggesting that organic acids play a key function in plant development and, in particular, in fruit development, maturation and ripening. Here, we critically review the connection between organic acids and the development of both climacteric and non-climacteric fruits. By analyzing the metabolic content of different fruits during their ontogenetic trajectory, we noticed that the content of organic acids in the early stages of fruit development is directly related to the supply of substrates for respiratory processes. Although different organic acid species can be found during fruit development in general, it appears that citrate and malate play major roles in this process, as they accumulate on a broad range of climacteric and non-climacteric fruits. We further highlight the functional significance of changes in organic acid profile in fruits due to either the manipulation of fruit-specific genes or the use of fruit-specific promoters. Despite the complexity behind the fluctuation in organic acid content during fruit development and ripening, we extend our understanding on the importance of organic acids on fruit metabolism and the need to further boost future research. We suggest that engineering organic acid metabolism could improve both qualitative and quantitative traits of crop fruits.

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Section: The Metabolic Behavior Of Organic Acids During Fruit Developmentioning

confidence: 99%

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

et al. 2018

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“…T-DNA insertion alleles demonstrated that AtARF8 is an important regulator of fruit initiation and that the disruption of its normal function induces parthenocarpy in Arabidopsis [ 14 ]. In tomato ( Solanum lycopersicon ), SlARF4 was demonstrated to be involved in sugar metabolism and cell wall architecture during tomato fruit development [ 36 , 37 ]. The transgenic antisense- OsARF1 rice showed extremely low growth, poor vigor, short curled leaves and sterility, suggesting that the OsARF1 is essential for growth in vegetative organs and seed development [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of AUXIN RESPONSE FACTOR gene family from Prunus sibirica and its expression analysis during mesocarp and kernel development

Niu

Deng

et al. 2018

BMC Plant Biol

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BackgroundAuxin response factors (ARFs) in auxin signaling pathway are an important component that can regulate the transcription of auxin-responsive genes involved in almost all aspects of plant growth and development. To our knowledge, the comprehensive and systematic characterization of ARF genes has never been reported in Prunus sibirica, a novel woody biodiesel feedstock in China.ResultsIn this study, we identified 14 PsARF genes with a perfect open reading frame (ORF) in P. sibirica by using its previous transcriptomic data. Conserved motif analysis showed that all identified PsARF proteins had typical DNA-binding and ARF domain, but 5 members (PsARF3, 8 10, 16 and 17) lacked the dimerization domain. Phylogenetic analysis of the ARF proteins generated from various plant species indicated that ARFs could be categorized into 4 major groups (Class I, II, III and IV), in which all identified ARFs from P. sibirica showed a closest relationship with those from P. mume. Comparison of the expression profiles of 14 PsARF genes in different developmental stages of Siberian apricot mesocarp (SAM) and kernel (SAK) reflected distinct temporal or spatial expression patterns for PsARF genes. Additionally, based on the expressed data from fruit and seed development of multiple plant species, we identified 1514 ARF-correlated genes using weighted gene co-expression network analysis (WGCNA). And the major portion of ARF-correlated gene was characterized to be involved in protein, nucleic acid and carbohydrate metabolic, transport and regulatory processes.ConclusionsIn summary, we systematically and comprehensively analyzed the structure, expression pattern and co-expression network of ARF gene family in P. sibirica. All our findings provide theoretical foundation for the PsARF gene family and will pave the way for elucidating the precise role of PsARF genes in SAM and SAK development.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1220-2) contains supplementary material, which is available to authorized users.

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“…These findings directed the functional roles of GH3 in reducing the IAA level and promoting fruit ripening. Studies with the regulation of auxin in transcript level indicated that an increase in firmness and prolonged shelf life was mediated by the down-regulation of the ARF4 gene in the tomato [ 61 ]. In our results, we also found that ARF genes were downregulated in the post-ripening samples, suggesting a role in fruit ripening.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Profiling to Understand Pre- and Post-Ripening Hormonal Regulations and Anthocyanin Biosynthesis in Early Ripening Apple Fruit

Onik

Lin

et al. 2018

Molecules

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The ‘Hongyu’ apple is an early ripening apple cultivar and usually used for fresh marketing. Due to the short ripening period, most of the fruit are harvested at the commercial maturity stage for proper marketing distribution and a longer shelf life. Fruit ripening involves delicate changes to its metabolic and physiological traits through well-organized synchronization of several hormones and regulatory steps. A clear understanding of these hormonal alterations is crucial for extending the period from commercial to physiological ripening. This study was intended to clarify the hormonal alterations and anthocyanin biosynthesis process prior to and immediate after, the harvesting of apple fruit considering the commercial maturity stage. Fruits harvested at 120 Days after flowering (DAF) (HY_4th) was considered as commercially ripened, 110 DAF (HY_3rd) as pre-ripening and 120 DAF followed by five days storage at 20 °C (HY_20 °C_5) as post-ripening samples. Three different stages of fruit were used for transcriptome assembly using RNA-Seq. Results revealed 9187 differentially expressed genes (DEGs) in the post-ripening samples, which was comparatively lower (922 DEGs) in the pre-ripening fruits. DEGs were subjected to Gene Ontology analysis and 31 categories were significantly enriched in the groups ‘biological process,’ ‘molecular function’ and ‘cellular component.’ The DEGs were involved in hormonal signaling pathways like ethylene, abscisic acid (ABA), auxin, gibberellin (GA), brassinosteroid (BR) and anthocyanin biosynthesis pathways such as PAL, 4CL, CHI, DFR, F3H, UFGT. Several transcription factors like the MADS-box gene, MYB, bHLH, NAC, WRKY and HSF were differentially expressed between the pre- and post-ripening fruits. Selected DEGs were subjected to gene expression analysis using quantitative RT-PCR (qRT-PCR) and the results were consistent with those of RNA-Seq. Our data suggested that in addition to ethylene, ABA and other hormones also play key roles in regulating apple fruit ripening and may interact with the ethylene signaling process. Additionally, our data provided an exhibition of the expression pattern of genes in the anthocyanin biosynthesis pathway.

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Under-expression of the Auxin Response FactorSl-ARF4improves post-harvest behavior of tomato fruits

Cited by 30 publications

References 18 publications

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

Identification of AUXIN RESPONSE FACTOR gene family from Prunus sibirica and its expression analysis during mesocarp and kernel development

Comparative Transcriptomic Profiling to Understand Pre- and Post-Ripening Hormonal Regulations and Anthocyanin Biosynthesis in Early Ripening Apple Fruit

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