Uncovering the Roles of Septins in Cilia

Palander, Oliva; El-Zeiry, Maha; Trimble, William S.

doi:10.3389/fcell.2017.00036

Cited by 37 publications

(32 citation statements)

References 55 publications

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“…21 At present, how septin ring works as diffusion barrier against free diffusion of membrane proteins and lipids remains unknown. 20,22,23 Cell adhesion apparatus in the basolateral domains of epithelial cells Finally, in this section, I introduce the findings about the roles of lipids in the formation of cell membrane structures involved in cell adhesion. In the basolatelral membrane domains, epithelial cells form membrane structures involved in the cell-cell or cell-matrix adhesion.…”

Section: Diffusion Barrier Of Cell Membranementioning

confidence: 99%

Roles of membrane lipids in the organization of epithelial cells: Old and new problems

Ikenouchi

2018

Tissue Barriers

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Epithelial cells have characteristic membrane domains. Identification of membrane proteins playing an important role in these membrane domains has progressed and numerous studies have been performed on the functional analysis of these membrane proteins. On the other hand, the precise roles of membrane lipids in the organization of these membrane domains are largely unknown. Historically, the concept of lipid raft arose from the analysis of lipid composition of the apical membrane, and it can be said that epithelial cells are an optimal experimental model for elucidating the functions of lipids. In this review, I discuss the role of lipids in the formation of epithelial polarity and in the formation of cell membrane structures of epithelial cells such as microvilli in the apical domain, cell-cell adhesion apparatus in the lateral domain and cell-matrix adhesion in the basal domain.

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Section: Diffusion Barrier Of Cell Membranementioning

confidence: 99%

Roles of membrane lipids in the organization of epithelial cells: Old and new problems

Ikenouchi

2018

Tissue Barriers

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“…Compared to yeast septins, animal septins have a much more variable cellular localization that changes with tissue type, developmental stage, and cell cycle state [20,21]. They are frequently found at curved regions of the plasma membrane such as the base of dendritic spines and cilia, where they provide a diffusion barrier [22][23][24]. However, animal septins also associate with flat regions of the cell cortex, where they influence the rigidity and contractility of the actin-myosin cortex within single cells [25][26][27] and within multicellular tissues [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Membrane binding controls ordered self-assembly of animal septins

Szuba

Bano

François

et al. 2020

Preprint

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Septins are conserved cytoskeletal proteins that regulate cell cortex mechanics. The mechanisms of their interactions with the plasma membrane remain poorly understood. Here we show by cell-free reconstitution that membrane binding requires electrostatic interactions of septins with anionic lipids and promotes the ordered self-assembly of fly septins into filamentous meshworks. Transmission electron microscopy reveals that both fly and mammalian septins form arrays of single and paired filaments. Atomic force microscopy and quartz crystal microbalance demonstrate that the fly filaments form mechanically rigid, 12 to 18 nm thick, double layers of septins. By contrast, C-terminally truncated septin mutants form 4 nm thin monolayers, indicating that stacking requires the C-terminal coiled coils on DSep2 and Pnut subunits. Our work shows that membrane binding is required for fly septins to form ordered arrays of single and paired filaments and provides new insights into the mechanisms by which septins may regulate cell surface mechanics.

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“…This diffusion barrier is critical for preventing the segregation of misfolded proteins from the mother to daughter compartment (Clay et al 2014; Chao et al 2014). Septins are also suggested to function as diffusion barriers in different mammalian cell processes such as compartmentalization of the sperm tail (Kissel et al 2005; Ihara et al 2005); the forming cilia (Hu et al 2010), although caution should be taken for this interpretation (Palander et al 2017); or during dendritic branching in neurons (Xie et al 2007; Tada et al 2007). This highlights the conservation of septin-based barriers beyond a specific model system.…”

Section: The Functional Significance Of the Septin Architecturesmentioning

confidence: 99%

Architecture, remodeling, and functions of the septin cytoskeleton

Marquardt

Chen

2018

Cytoskeleton

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The septin family of proteins has fascinated cell biologists for decades due to the elaborate architecture they adopt in different eukaryotic cells. Whether they exist as rings, collars, or gauzes in different cell types and at different times in the cell cycle illustrates a complex series of regulation in structure. While the organization of different septin structures at the cortex of different cell types during the cell cycle has been described to various degrees, the exact structure and regulation at the filament level are still largely unknown. Recent advances in fluorescent and electron microscopy, as well as work in septin biochemistry, have allowed new insights into the aspects of septin architecture, remodeling, and function in many cell types. This mini-review highlights many of the recent findings with an emphasis on the budding yeast model.

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Uncovering the Roles of Septins in Cilia

Cited by 37 publications

References 55 publications

Roles of membrane lipids in the organization of epithelial cells: Old and new problems

Roles of membrane lipids in the organization of epithelial cells: Old and new problems

Membrane binding controls ordered self-assembly of animal septins

Architecture, remodeling, and functions of the septin cytoskeleton

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