Uncovering the metabolic pathway of novel Burkholderia sp. for efficient triclosan degradation and implication: Insight from exogenous bioaugmentation and toxicity pressure

Qiu, Lan; Guo, Xiaoyuan; Liang, Zhiwei; Lu, Qihong; Wang, Shanquan; Shim, Hyungbo

doi:10.1016/j.envpol.2023.122111

Cited by 4 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since 2001, when Sayler et al isolated the first TCS-degrading bacterium, Sphingomonas sp. RD1, from activated sludge in a wastewater treatment plant, a total of 21 TCS-degrading microorganisms have been reported. ,, The degradation pathways of TCS include anaerobic degradation and aerobic degradation. The initial step in TCS anaerobic degradation is dechlorination. , Most TCS-degrading bacteria degrade TCS through aerobic degradation .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation

Yin,

Ren,

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

The emerging contaminant triclosan (TCS) is widely distributed both in surface water and in wastewater and poses a threat to aquatic organisms and human health due to its resistance to degradation. The dioxygenase enzyme TcsAB has been speculated to perform the initial degradation of TCS, but its precise catalytic mechanism remains unclear. In this study, the function of TcsAB was elucidated using multiple biochemical and molecular biology methods. Escherichia coli BL21(DE3) heterologously expressing tcsAB from Sphingomonas sp. RD1 converted TCS to 2,4-dichlorophenol. TcsAB belongs to the group IA family of twocomponent Rieske nonheme iron ring-hydroxylating dioxygenases. The highest amino acid identity of TcsA and the large subunits of other dioxygenases in the same family was only 35.50%, indicating that TcsAB is a novel dioxygenase. Mutagenesis of residues near the substrate binding pocket decreased the TCS-degrading activity and narrowed the substrate spectrum, except for the TcsA F343A mutant. A meta-analysis of 1492 samples from wastewater treatment systems worldwide revealed that tcsA genes are widely distributed. This study is the first to report that the TCS-specific dioxygenase TcsAB is responsible for the initial degradation of TCS. Studying the microbial degradation mechanism of TCS is crucial for removing this pollutant from the environment.

show abstract

Section: Introductionmentioning

confidence: 99%

“…RD1, from activated sludge in a wastewater treatment plant, 14 a total of 21 TCS-degrading microorganisms have been reported. 13,15,16 The degradation pathways of TCS include anaerobic degradation and aerobic degradation. The initial step in TCS anaerobic degradation is dechlorination.…”

Section: Introductionmentioning

confidence: 99%

Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation

Yin,

Ren,

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

Propane Monooxygenases in Soil Associated Metagenomes Align Most Closely to those in the Genera Kribbella, Amycolatopsis, Bradyrhizobium, Paraburkholderia and Burkholderia

Cupples

2024

Curr Microbiol

View full text Add to dashboard Cite

Current strategies on bioremediation of personal care products and detergents: Sustainability and life cycle assessment

Saravanan,

Thamarai,

Deivayanai

et al. 2024

Chemosphere

View full text Add to dashboard Cite

Uncovering the metabolic pathway of novel Burkholderia sp. for efficient triclosan degradation and implication: Insight from exogenous bioaugmentation and toxicity pressure

Cited by 4 publications

References 61 publications

Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation

Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation

Propane Monooxygenases in Soil Associated Metagenomes Align Most Closely to those in the Genera Kribbella, Amycolatopsis, Bradyrhizobium, Paraburkholderia and Burkholderia

Current strategies on bioremediation of personal care products and detergents: Sustainability and life cycle assessment

Contact Info

Product

Resources

About