Uncovering the diverse hosts of tigecycline resistance gene tet(X4) in anaerobic digestion systems treating swine manure by epicPCR

Dai, Shiting; He, Qing; Han, Ziming; Shen, Wei; Deng, Ye; Wang, Yang; Qiao, Wei; Yang, Min; Zhang, Yu

doi:10.1016/j.wroa.2023.100174

Cited by 6 publications

(2 citation statements)

References 75 publications

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“…A growing number of studies have reported that any environment has the potential to be a host for ARGs, with human, animal, and environmental components interacting and contributing directly or indirectly to the spread of ARGs ( Dai et al, 2023 ; Ramatla et al, 2023 ). Therefore, ARGs are considered to be a new environmental pollutant ( Zhu et al, 2017 , 2018 ; Zhang et al, 2020 , 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Decoding the enigma: unveiling the molecular transmission of avian-associated tet(X4)-positive E. coli in Sichuan Province, China

Zhang,

Wen,

Wang

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Decoding the enigma: unveiling the molecular transmission of avian-associated tet(X4)-positive E. coli in Sichuan Province, China

Zhang,

Wen,

Wang

et al. 2023

Poultry Science

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“…The fusion products are then isolated and sequenced in parallel. So far, the method has been applied to reveal bacterial hosts of various genes associated with AMR, including intI1 and sul1, in environmental samples [17][18][19][20][21]. A variant of epicPCR was used to identify bacterial hosts of beta-lactamases in human gut microbiomes [22].…”

Section: Introductionmentioning

confidence: 99%

Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach

Knecht,

Hinkel,

Mäusezahl

et al. 2023

Water

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Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management of the global AMR crisis. Untreated and even treated wastewaters are important sources that release AMR into the environment. Methodologically, it is difficult to generate a comprehensive in situ profile of antibiotic resistance gene hosts. Here, we used epicPCR (emulsion, paired isolation, and concatenation PCR) as a cultivation-independent method to reveal the host profiles of the AMR indicator genes intI1, sul1, sul2, and dfrA1 in two constructed wetlands treating municipal wastewater. Overall, the epicPCR analysis revealed a profile of AMR indicator gene hosts that is consistent with literature data from cultivation-based approaches. Most carriers of antibiotic resistance (AR) genes and likely of class 1 integrons belonged to the Gammaproteobateria, particularly the Burkholderiaceae and Rhodocyclaceae families, followed by members of the Campylobacterota, Desulfobacterota, and Firmicutes. The analysis also identified several novel hosts for the indicator genes widely distributed in the wetlands, including the genera Legionella and Ralstonia. Therefore, the application of epicPCR has produced an expanded insight into the in situ indicator gene host profile, while highlighting the role of the environment as a reservoir for AMR.

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Insights into response mechanism of anaerobic digestion of waste activated sludge to particle sizes of zeolite

Tang

Zhang

Yao

et al. 2023

Bioresource Technology

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Uncovering the diverse hosts of tigecycline resistance gene tet(X4) in anaerobic digestion systems treating swine manure by epicPCR

Cited by 6 publications

References 75 publications

Decoding the enigma: unveiling the molecular transmission of avian-associated tet(X4)-positive E. coli in Sichuan Province, China

Decoding the enigma: unveiling the molecular transmission of avian-associated tet(X4)-positive E. coli in Sichuan Province, China

Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach

Insights into response mechanism of anaerobic digestion of waste activated sludge to particle sizes of zeolite

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