Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis

Abstract

Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes...

Exploring immunoregulatory properties of a phenolic-enriched maple syrup extract through integrated proteomics and in vitro assays

“…30,31 In a previous study, we exclusively utilized the DIA strategy for the discovery of proteomics. 23 However, a notable limitation of DIA workflows is the multiplexed spectra within each MS 2 scan, making accurate peptide identification challenging. 32 Herein, we strategically integrated the DDA proteomics into the DIA analysis process.…”

“…The mice were provided a 2020X Teklad Global Soy Protein-Free Extruded Rodent Diet as we previously reported. 23 All experimental procedures undertaken in this study align with those detailed in our earlier publications. 13,23 In detail, three groups including the control group, model, and MSX groups were employed in this study.…”

“…23 All experimental procedures undertaken in this study align with those detailed in our earlier publications. 13,23 In detail, three groups including the control group, model, and MSX groups were employed in this study. For the control group, mice were administered an intraperitoneal (i.p.)…”

“…The spleen tissues used in this study were obtained from an animal study we previously reported. 23 Specifically, we utilized an LPS-induced peritonitis model in mice where the optimal MSX dose (0.05%) was selected in a dose-finding study, followed by a DIA proteomic analysis of the kidney and liver. The study was conducted in strict adherence to the guidelines outlined in the “Guide for the Care and Use of Laboratory Animals,” 8th Edition, as released by the National Institutes of Health (NIH), the “Public Health Service Policy on Humane Care and Use of Laboratory Animals” (PHS Policy), and the “Animal Welfare Regulations” (AWRs) set forth by the United States Department of Agriculture (USDA).…”

“…Recently, our group elucidated the anti-inflammatory mechanisms of MSX in an LPS-induced peritonitis mouse model, utilizing data-independent acquisition (DIA) proteomics to gain a comprehensive understanding of its action. 23 Building upon the foundation of that study, the current research aims to evaluate MSX's potential immunoregulatory effects in the context of splenic inflammation induced by LPS. To facilitate this investigation, we have taken several strategic steps.…”