Uncovering PGPB Vibrio spartinae inoculation-triggered physiological mechanisms involved in the tolerance of Halimione portulacoides to NaCl excess

Mateos‐Naranjo, Enrique; López‐Jurado, Javier; Redondo‐Gómez, Susana; Pérez-Romero, Jesús Alberto; Glick, Bernard R.; Rodríguez‐Llorente, Ignacio D.; Pajuelo, Eloísa; Echegoyan, Almudena; Mesa‐Marín, Jennifer

doi:10.1016/j.plaphy.2020.05.034

Cited by 11 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The traits evaluated from the chlorophyll fluorescence measurements confirmed that H. portulacoides is highly salt-tolerant, as expected from the findings in previous studies [34,35], although at the high tested concentration (600 mM NaCl) already some signs of stress could be found. Nevertheless, the most noticeable differences were detected when comparing the non-inoculated and inoculated plants' responses to the NaCl treatments.…”

Section: Discussionsupporting

confidence: 87%

Bioaugmentation Improves Phytoprotection in <em>Halimione Portulacoides </em>Exposed to Mild Salt Stress: Perspectives for Salinized Soil Restoration

Carreiras¹,

Caçador²,

Duarte³

2022

Preprint

View full text Add to dashboard Cite

Rhizosphere bacteria have a decisive influence on plant ionic adjustment, as well as in ameliorating plant growth under an array of stress situations. Plant growth-promoting rhizobacteria (PGPR) colonize the rhizosphere of plants and promote plant growth through mechanisms such as solubilization of mineral phosphates, biological N2 fixation, production of siderophores and phytohormones, and can induce systemic resistance in the plant. This can be of extreme importance when considering the restoration of salinized grounds by halophytic species. This present work aims to evaluate the physiological fitness and phytoprotection improvement by salt marsh PGPR in Halimione portulacoides under mild and severe salt stress. Plants inoculated with PGPR-consortium showed higher photochemical performances, improved antioxidant response, and promotion of osmotic balance traits, that boosted the individual’s ability to cope with mild salt stress. All these changes are also in line with the differential elemental profiles (Na, K, and Ca) observed in the different plant tissues. Even under severe salt stress, some physiological traits were improved when compared to the non-inoculated individuals. The results developed under this work, point out an important role of bioaugmentation in promoting plant fitness and improving salt tolerance, with a great potential for applications in seawater agriculture, restoration, and bio-reclamation of salinized soils.

show abstract

Section: Discussionsupporting

confidence: 87%

Bioaugmentation Improves Phytoprotection in <em>Halimione Portulacoides </em>Exposed to Mild Salt Stress: Perspectives for Salinized Soil Restoration

Carreiras¹,

Caçador²,

Duarte³

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Additionally, 40, 60, 80, 100, and 120 days after the application of experimental treatments, leaf instantaneous gas exchange and photochemical traits characteristics were recorded in fully developed leaves from each inoculation and phosphorus fertilization treatment combination (n = 10) using an infrared gas analyzer (LI-6800-01, LICOR Inc., Lincoln, NE, USA) and a fluorimeter (FMS-2; Hansatech Instruments Ltd., King's Lynn, UK), respectively. Thus, net photosynthetic rate (A N ), stomatal conductance (g s ), intercellular CO 2 concentration (C i ) and intrinsic water use efficiency ( i WUE), actual efficiency of the photosystem II (Φ PSII ), and the electron transport rate (ETR) were measured at midday between 11:00 and 13:00 pm at 1000 µmol photon m −2 s −1 (with 15% blue light), 2.0-3.0 kPa vapor pressure, 50 ± 1% air relative humidity, 400 µmol CO 2 mol −1 , and 25 • C. Finally, maximum quantum efficiency of PSII photochemistry (F v /F m ) was obtained in dark-adapted leaves according to the protocol described by Mateos-Naranjo et al [37].…”

Section: Plant Growth and Physiological Traits Analysismentioning

confidence: 99%

Biofertilization with PGP Bacteria Improve Strawberry Plant Performance under Sub-Optimum Phosphorus Fertilization

et al. 2023

Self Cite

View full text Add to dashboard Cite

Biofertilization with plant growth-promoting bacteria (PGPB) could optimize chemical fertilization for strawberry crop cultivation. A greenhouse study was arranged to assess the impact of an isolated PGPB consortium from halophytes on strawberry development, physiological traits, and nutritional balance subjected to two phosphorus fertilization limitation treatments (with and without insoluble phosphorus form application). Biofertilization had a positive effect on strawberry development. Thus, shoot and root biomass was c. 20 and 32% higher in inoculated plants grown with insoluble phosphorus. This effect was mediated by a positive bacterial impact on plant carbon absorption capacity and water use efficiency, through a reduction in CO2 diffusional and biochemical photosynthesis limitation. Thus, net photosynthetic rate and intrinsic water use efficiency showed increments of 21–56% and 14–37%, respectively. In addition, inoculation led to a better efficiency of the plant photochemical apparatus, as indicated by the invariable higher PSII photochemistry parameters. Furthermore, these effects correlated with improved nutritional balance of phosphorus and nitrogen, which was directly related to the beneficial impact on carbon metabolism and, consequently, on strawberries’ growth. In conclusion, we can recommend the biofertilization based on PGPB for achieving more efficient strawberry P fertilization management practices, providing high efficiency in yields.

show abstract

Section: Methodsmentioning

confidence: 99%

“…On the other hand, photochemical traits of plants were obtained in fully developed leaves from each experimental treatment (n = 10 replicates per treatment) coupled to a multiphase flash fluorometer (LI-6800-01A, LICOR Inc., Lincoln, NE, USA) with an infrared gas analyzer. Furthermore, the maximum quantum efficiency of PSII photochemistry (F v /F m ) and non-photochemical quenching (NPQ) was obtained in leaves adapted to light and dark (n = 10 replicates per treatment) with a portable fluorimeter FMS-2 (Hansatech Instruments Ltd., King's Lynn, UK) according to the protocol described by Mateos-Naranjo et al (2020). Finally, to complete the fluorometry study, delayed fluorescence of leaves of plants subjected to different nitrogen fertilization treatments was detected using a plant imaging system (NightShade LB 985, Berthold Technologies, Germany) equipped with a deeply cooled CCD camera (n = 5 replicates per treatment).…”

Section: Analysis Of Plant Growth and Physiological Traitsmentioning

confidence: 99%

Exploring through the use of physiological and isotopic techniques the potential of a PGPR-based biofertilizer to improve nitrogen fertilization practices efficiency in strawberry cultivation

García-López,

Redondo-Gómez,

Flores-Duarte

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

The use of microorganisms as a biofertilizer in strawberry has focused mainly on pathogen biocontrol, which has led to the underestimation of the potential of microorganisms for the improvement of nutritional efficiency in this crop. A study was established to investigate the impact of a plant growth-promoting rhizobacteria (PGPR) based biofertilizer integrated by self-compatible stress tolerant strains with multiple PGP properties, including atmospheric nitrogen fixation, on strawberry (Fragaria × ananassa cv. Rociera) tolerance to N deficiency in terms of growth and physiological performance. After 40 days of nitrogen fertilization shortage, inoculated plants were able to maintain root development and fertility structures (i.e. fruits and flowers) at a level similar to plants properly fertilized. In addition, inoculation lessened the negative impact of nitrogen deficiency on leaves’ dry weight and relative water content. This effect was mediated by a higher root/shoot ratio, which would have allowed them to explore larger volumes of soil for the acquisition of water. Moreover, inoculation was able to buffer up to 50% of the reduction in carbon assimilation capacity, due to its positive effect on the diffusion efficiency of CO2 and the biochemical capacity of photosynthesis, as well as on the activity of photosystem II light harvesting. Furthermore, the higher leaf C/N ratio and the maintained δ15N values close to control plants were related to positive bacterial effects at the level of the plant nutritional balance. Despite these positive effects, the application of the bacterial inoculum was unable to completely counteract the restriction of fertilization, being necessary to apply a certain amount of synthetic fertilizer for the strawberry nutrition. However, according to our results, the complementary effect of this PGPR-based biofertilizer could provide a higher efficiency in environmental and economic yields on this crop.

show abstract

Uncovering PGPB Vibrio spartinae inoculation-triggered physiological mechanisms involved in the tolerance of Halimione portulacoides to NaCl excess

Cited by 11 publications

References 40 publications

Bioaugmentation Improves Phytoprotection in <em>Halimione Portulacoides </em>Exposed to Mild Salt Stress: Perspectives for Salinized Soil Restoration

Bioaugmentation Improves Phytoprotection in <em>Halimione Portulacoides </em>Exposed to Mild Salt Stress: Perspectives for Salinized Soil Restoration

Biofertilization with PGP Bacteria Improve Strawberry Plant Performance under Sub-Optimum Phosphorus Fertilization

Exploring through the use of physiological and isotopic techniques the potential of a PGPR-based biofertilizer to improve nitrogen fertilization practices efficiency in strawberry cultivation

Contact Info

Product

Resources

About