Uncoupling of DNA and histone synthesis prior to prophase I of meiosis in the cricket Grillus (Acheta) domesticus L

Bogdanov, Yu. F.; Ляпунова, Н. А.; Sherudilo, A.I.; Антропова, Е. Н.

doi:10.1016/0014-4827(68)90547-8

Cited by 25 publications

(8 citation statements)

References 18 publications

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“…The activity of these proteins during meiotic prophase and the control of supercoiling in meiotic chromatin are exciting topics for future research. Observations concerning the special qualities of the premeiotic S phase (Callan and Taylor, 1968;Callan, 1972;Callan, 1973; Hotta and Stern, 1971b), the unusual properties of meiotic histones (Sheridan and Stern, 1967;Bogdanov et at., 1968;Strokov et at., 1973;Nadler, 1976), and the altered nucleosome arrangement in meiotic chromatin may also be relevant to understanding how the careful control of meiotic chromosome condensation is achieved. Throughout this discussion, the occurrence of recombination at a limited number of specific sites in the genome has been emphasised.…”

Section: Discussionmentioning

confidence: 99%

Biochemical analysis of genetic recombination in eukaryotes

Pukkila

1977

Heredity

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SUMMARYRecent studies concerning molecular mechanisms of genetic recombination in eukaryotes are reviewed. Since many of these studies have focused on the testable predictions arising from the hybrid DNA theory of genetic recombination, this theory is summarised. Experiments to determine the time of meiotic crossing-over and the structure of the synaptonemal complex which facilitates meiotic crossing-over are described. Investigations of DNA nicking and repair events implicated in recombination are discussed. Properties of proteins which may facilitate hybrid DNA formation, and biochemical evidence for hybrid DNA formation are presented. Finally, a nuclease which has been implicated in gene conversion is described.STUDIES of the molecular events which result in recombination between chromosomes have been initiated in a wide variety of eukaryotic organisms. Since the meiocyte is a highly specialised cell which will produce specialised haploid progeny, the problem of identifying biochemical events which are strictly related to genetic recombination in the meiocyte are formidable indeed. In higher eukaryotes, the task of analysing genetic exchanges is further complicated by the striking evolutionary conservation of the number of reciprocal exchanges which occur in each meiosis. Although the amount of DNA can vary by orders of magnitude, the number of exchanges has remained quite limited.The hybrid DNA theory as proposed by Holliday (1964) and Whitehouse and Hastings (1965) has become the focal point for experimental approaches. The scheme proposed by Holliday (1964) is still compatible with the available genetic data, and thus will be used as a basis for the discussion, in Section 1, of likely biochemical events during meiotic recombination. The first requirement which must be met to study the biochemical predictions which arise from this theory is to determine the time of meiotic crossing-over. The evidence, discussed in Section 2, strongly suggests that genetic exchange occurs during the pachytene stage of meiotic prophase. The prominent feature of the pachytene nucleus, which provides the framework for crossingover, is the synaptonemal complex, whose structure is reviewed in Section 3.DNA nicking and repair events implicated in hybrid DNA formation and recombination are evaluated in Section 4, and properties of DNA binding proteins which may facilitate this process are reviewed in Section 5. Biochemical evidence for hybrid DNA formation is presented in Section 6 and a nuclease implicated in gene conversion is described in Section 7. 's model (1964) nicks are introduced in single strands of the same polarity at corresponding sites in two homologous chromatids. These strands unravel, exchange positions, and reanneal with the complementary unnicked strand in the homologous chromatid, forming a half-chromatid chiasma and generating hybrid DNA on the two chromatids. The following events do not necessarily occur in the order in which they are described. The nicks at the origin of the hybrid DNA are ligated. The ...

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Section: Discussionmentioning

confidence: 99%

Biochemical analysis of genetic recombination in eukaryotes

Pukkila

1977

Heredity

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“…After staining, preparations were washed in the same buffer for three minutes and dehydrated in absolute tertiary butanol and xylene to mount in Canada balsam. Other details were as described previously (ANTROPOVA and BoGDANOV, 1970;BoGDANOV et al 1968).…”

Section: Methodsmentioning

confidence: 99%

“…While DNA synthesis is completed during the premeiotic period, the increase in histone content in the nucleus continues until the pachytene stage. For example, in early pachytene of a house cricket and a bug, Pyrrhocoris apterus, the amount of histone in the nucleus increases by 30% (BoGDANOV et al 1968;ANTROPOVA and BOGDANOV 1970). In contrast, during the mitotic cycle histone synthesis is synchronized with the doubling of DNA and both these syntheses are completed in the interphase.…”

Section: Introductionmentioning

confidence: 99%

“…The methods employed were similar to those used in the previous studies of histone and DNA in meiosis (ANTROPOVA and BoGDANOV 1970;BoGDANOV et al 1968).…”

Section: Introductionmentioning

confidence: 99%

“…Recently two of us have demonstrated (ANTROPOVA and BoGDANOV 1970;BoGDANOV et al 1968) that during the transition of cells from mitosis to meiosis the doubling of histone lags behind the doubling of DNA. While DNA synthesis is completed during the premeiotic period, the increase in histone content in the nucleus continues until the pachytene stage.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytophotometric Studies of Histone and DNA in the Mitotic Cycle of Tulip Endosperm Nuclei

Антропова¹,

Petrova

Bogdanov³

1970

Caryologia

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Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study

Bertout¹

1983

J. Exp. Zool.

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The development of DNA and RNA synthesis in the germ cell population was studied after a 3H-thymidine or 3H-uridine pulse at each stage of spermatogenesis. The autoradiographic results show that the first sign (after 3 days in vitro) of cellular changes is an increase in RNA synthesis which reaches a maximum at day 5. DNA replication (premeiotic S phase) occurred at day 7, then cells entered meiotic prophase (day 9). Meiotic divisions and spermiogenesis occurred after 11 days. Silver grain counts permit the conclusion that RNA synthesis is clearly higher during premeiotic interphase (days 3-7) than during spermatogonial proliferation (day 0). It appears therefore that male meiotic differentiation in Nereidae is accompanied by increased RNA synthesis.

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Uncoupling of DNA and histone synthesis prior to prophase I of meiosis in the cricket Grillus (Acheta) domesticus L

Cited by 25 publications

References 18 publications

Biochemical analysis of genetic recombination in eukaryotes

Biochemical analysis of genetic recombination in eukaryotes

Cytophotometric Studies of Histone and DNA in the Mitotic Cycle of Tulip Endosperm Nuclei

Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study

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