Uncoupling heart cell specification and migration in the simple chordate<i>Ciona intestinalis</i>

Davidson, Brad; Shi, Weixing; Levine, Michael

doi:10.1242/dev.02051

Cited by 98 publications

(125 citation statements)

References 25 publications

(24 reference statements)

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“…In addition, the medaka 2.8 kb mesp-b enhancer can substitute for the mouse Mesp2 350 bp enhancer, suggesting the mesp-b anterior PSM enhancer is conserved in vertebrates despite differences in size (Yasuhiko et al, 2008). In Ciona T box binding sites found upstream of mesp are critical for expression of a reporter in the heart/muscle progenitor cells (Davidson et al, 2005), whilst in medaka and mouse T-box sites upstream of mesp-b/Mesp2 are also required for reporter expression in stripes at the anterior PSM (Terasaki et al, 2006, Yasuhiko et al, 2008. Our binding site analysis indicates that clusters of T-box sites are present upstream of all mesp-bs, and that in medaka, fugu, tetraodon and stickleback these sites fall within or close to regions of high conservation.…”

Section: Discussionmentioning

confidence: 99%

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Identification and expression analysis of two novel members of the Mesp family in zebrafish

Cutty¹,

Fior²,

Henriques³

et al. 2012

Int. J. Dev. Biol.

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Mesp proteins play crucial roles in the formation of heart, vasculature and somites during vertebrate embryogenesis. We have used phylogenetic and genomic analysis, combined with qRT-PCR and in situ hybridization, to characterize two novel additional mesp genes in zebrafish, mesp-ab and mesp-bb, and describe their expression pattern in wild type and segmentation mutants. Both mesp-ab and mesp-bb are expressed in early mesoderm with mesp-ab expression starting during late blastula stages and mesp-bb expression initiating later, at the end of gastrulation. During somitogenesis, both mesp genes are expressed dynamically in the anterior presomitic mesoderm. mesp-ab is expressed in presumptive somites S-I and S-II, while mesp-bb is detected in S-I, S-II and S0, with expression restricted to the rostral compartment of presumptive somites. We show that the segmentation clock program regulates expression of these newly identified zebrafish mesp genes in a similar manner to their ohnologs, mesp-aa and mesp-ba. We also present evidence that zebrafish, minnow and salmon retained these additional mesp genes after the teleost whole genome duplication, while medaka, stickleback, fugu and tetraodon did not. Finally we show that although expression and regulation of zebrafish mesp genes appears highly comparable, there is no conservation in non-coding regions with other teleosts. In this study we have completed the description of the Mesp family in zebrafish, which will enable correct genome annotation and facilitate further functional studies on the role of these proteins in zebrafish.

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Section: Discussionmentioning

confidence: 99%

“…1C). However the genomic sequence in this area is been shown to have a role in heart formation (Davidson et al, 2005, Satou et al, 2004, suggesting a conserved ancestral role for mesp genes.…”

Section: Syntenic Analysis Of Mesp Proteinsmentioning

confidence: 99%

Identification and expression analysis of two novel members of the Mesp family in zebrafish

Cutty¹,

Fior²,

Henriques³

et al. 2012

Int. J. Dev. Biol.

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“…The Mesp>FoxF:VP16 and Mesp>FoxF:WRPW fusion genes were derived from the previously reported Mesp>Mesp:VP16 and Mesp>Mesp:WRPW fusion genes (Davidson et al, 2005), by replacing the Mesp bHLH domain with the FoxF-DBD fragment.…”

Section: Molecular Cloningmentioning

confidence: 99%

“…However, it has been possible to uncouple TVC migration from cardiac muscle induction by targeted expression of a constitutively activated form of Mesp, the Mesp:VP16 fusion protein, in the B7.5 lineage (Davidson et al, 2005). TVCs are sometimes arrested in the anterior tail, and differentiate into disorganized aggregates of contractile cardiomyocytes at an ectopic location in the juvenile.…”

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confidence: 99%

FoxFis essential for FGF-induced migration of heart progenitor cells in the ascidianCiona intestinalis

et al. 2007

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Heart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK-mediated activation of the transcription factor Ets1/2 is required for heart tissue specification and cell migration. We found that FoxF is one of the first genes to be activated in heart precursors in response to FGF signaling. We identified the FoxF minimal heart enhancer and used a cis-trans complementation test to show that Ets1/2 can interact with the FoxF enhancer in vivo. Next, we found that FoxF function is required downstream and in parallel to the FGF/MAPK/Ets cascade for cell migration. In addition, we demonstrated that targeted expression of a dominant-negative form of FoxF inhibits cell migration but not heart differentiation, resulting in a striking phenotype: a beating heart at an ectopic location within the body cavity of juveniles. Taken together, our results indicate that FoxF is a direct target of FGF signaling and is predominantly involved in the regulation of heart cell migration.

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“…1 A), and Tbx24 also binds to the Mesp2 upstream region (data not shown). Recently, Davidson et al (22) reported that, during heart development in the simple chordate Ciona intestinalis, a Mesp homolog is also expressed in a Tbx6-dependent manner. Comparing genomic sequences among Ciona, mouse, and zebrafish, the authors identified multiple Tbx6 binding sites in the upstream sequence of Ciona Mesp homolog.…”

Section: Tbx6 Binds To Cis-regulatory Elements Of the Mesp2 Gene Andmentioning

confidence: 99%

Tbx6-mediated Notch signaling controls somite-specific Mesp2 expression

Yasuhiko

Haraguchi²,

Kitajima³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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142

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Mesp2 is a transcription factor that plays fundamental roles in somitogenesis, and its expression is strictly restricted to the anterior presomitic mesoderm just before segment border formation. The transcriptional on-off cycle is linked to the segmentation clock. In our current study, we show that a T-box transcription factor, Tbx6, is essential for Mesp2 expression. Tbx6 directly binds to the Mesp2 gene upstream region and mediates Notch signaling, and subsequent Mesp2 transcription, in the anterior presomitic mesoderm. Our data therefore reveal that a mechanism, via Tbx6-dependent Notch signaling, acts on the transcriptional regulation of Mesp2. This finding uncovers an additional component of the interacting network of various signaling pathways that are involved in somitogenesis.enhancer ͉ transgenic mouse ͉ RBPJ ͉ luciferase assay

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Uncoupling heart cell specification and migration in the simple chordateCiona intestinalis

Cited by 98 publications

References 25 publications

Identification and expression analysis of two novel members of the Mesp family in zebrafish

Identification and expression analysis of two novel members of the Mesp family in zebrafish

FoxFis essential for FGF-induced migration of heart progenitor cells in the ascidianCiona intestinalis

Tbx6-mediated Notch signaling controls somite-specific Mesp2 expression

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