Single-layer FeSe films with extremely expanded in-plane lattice constant of 3.99±0.02 Å are fabricated by epitaxially growing FeSe/Nb:SrTiO 3 /KTaO 3 heterostructures, and studied by in situ angle-resolved photoemission spectroscopy. Two elliptical electron pockets at the Brillion zone corner are resolved with negligible hybridization between them, indicating the symmetry of the low energy electronic structure remains intact as a free-standing single-layer FeSe, although it is on a substrate. The superconducting gap closes at a record high temperature of 70 K for the iron based superconductors. Intriguingly, the superconducting gap distribution is anisotropic but nodeless around the electron pockets, with minima at the crossings of the two pockets. Our results put strong constraints on the current theories, and support the coexistence of both even and odd parity spin-singlet pairing channels as classified by the lattice symmetry. [3][4][5]. For these systems, weak coupling theories based on spin-fluctuations predict a dwave pairing symmetry [6,7]. However, it is inconsistent with the isotropic superconducting gap observed by angle resolved photoemission spectroscopy (ARPES) [2,3,8,9], together with evidences for nodeless superconducting gap from specific heat [10], nuclear magnetic resonance [11], etc. On the other hand, the sign preserving s-wave pairing symmetry [12][13][14][15] could not account for the spin-resonance mode found in Rb x Fe 2−y Se 2 by inelastic neutron scattering [16], which suggests the sign change of the superconducting order parameter on different Fermi surface sections [17].To explain the sign changing isotropic gap in e-FeHTSs, several novel pairing scenarios were proposed. For example, it is argued in the bonding-antibonding s ± pairing scenario that with strong hybridization between electron pockets, the two reconstructed electron pockets can have different signs [18]. A further study suggested that this pairing likely coexists with the d-wave to form an s + id-wave pairing symmetry [19]. More recently, the importance of the parity of the 2-Fe unit cell has been emphasized [20], and it has been proposed that there are even and odd parity s-wave spin singlet pairing states, and the coexistence of both states gives a fully gapped state with varied signs in different Fermi surface sections [21,22]. The hybridization between the two electron pockets is not necessary in this scenario. So far, these scenarios could not be convincingly tested, since the detailed structure of the two electron pockets could not be resolved in all known e-FeHTSs.Two recent ARPES studies have found a gap in singlelayer FeSe/STO, which closes at 65 K and suggests a possible record high superconducting transition temperature (T c ) of 65 K for FeHTSs [4,5]; or at least, it is the pair-formation temperature record, if the superconducting transition there is a two dimensional Berezinskii-Kosterlitz-Thouless (BKT) type. Particularly, our previous ARPES study has found that the high T c in single-layer FeSe/STO is in...