Unconjugated bilirubin ameliorates the inflammation and digestive protease increase in TNBS-induced colitis

Zhou, Jinan; Jiang, Meng; Yang, Xiaoshan; Liu, Yuanli; Guo, Junyu; Zheng, Jiadong; Qu, Yilin; Song, Yu; Li, Rongyan; Qin, Xiaofa; Wang, Xiuhong

doi:10.3892/mmr.2017.6825

“…In addition, evidence from experimental studies in vitro and in vivo strongly supports the data obtained in human studies. A study on the treatment of experimental colitis in a rat model showed that UCB treatment reduced the inflammatory response induced by trinitrobenzene sulfonic acid and had a strong anti-inflammatory effect [34]. Hypochlorous acid (HOCl), an oxidant produced by myeloperoxidase (MPO), can induce protein and lipid oxidation.…”

Section: Discussionmentioning

confidence: 99%

Association of total bilirubin and indirect bilirubin content with metabolic syndrome among Kazakhs in Xinjiang

Hao

¹

,

Guo

²

,

Ma

³

et al. 2020

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Background: Some studies have shown that a high level of bilirubin is a protective factor against metabolic syndrome (MS), while a high level of transaminase is a risk factor for MS. However, the existing results are inconsistent and few cohort studies have been published. Methods: Using an ambispective cohort study, 565 Kazakhs from Xinjiang, China were selected as the study subjects. The baseline serum bilirubin and transaminase levels of the subjects were divided into quartiles and the relationship between these values and the incidence of MS was analyzed. The definition of MS was based on the Joint Interim Statement (JIS) diagnostic criteria. Results: The average follow-up time for the subjects was 5.72 years. The cumulative incidence of MS was 36.11% (204 of the 565 subjects), and the incidence density was 63.10/1000 person-years. Multivariate Cox regression analysis showed that the levels of total bilirubin (TBIL) and indirect bilirubin (IBIL) were negatively correlated with the occurrence of MS, Compared to the lowest quartile level (Q1), the hazard ratios of MS the TBIL levels at the Q2-Q4 quartiles were: 0.47 (0.31-0.71), 0.53 (0.35-0.79), and 0.48 (0.32-0.72), respectively, while IBIL levels at the Q2-Q4 quartiles showed an MS hazard ratio of 0.48 (0.32-0.72), 0.54(0.36-0.81), and 0.52 (0.35-0.77), respectively, all at a 95% confidence level. However, no relationship was found between transaminase levels and the incidence of MS. Conclusion: Serum TBIL and IBIL levels were negatively correlated with the incidence of MS in a Kazakh population in China.

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“…The disease activity index (DAI) was calculated for each animal by adding together the scores for body weight, stool consistency, and stool blood as listed in Supplementary Table 1 and described in our previous studies (29).…”

Section: Disease Activity Indexmentioning

confidence: 99%

Sucralose Promotes Colitis-Associated Colorectal Cancer Risk in a Murine Model Along With Changes in Microbiota

Li

¹

,

Liu

²

,

Li

³

et al. 2020

Self Cite

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Sucralose is a calorie-free high-intensity artificial sweetener that is widely used in thousands of foods and beverages all over the world. Although it was initially regarded as a safe, inert food additive, its adverse effect on gut microbiota and health has drawn more and more attention as evidence accumulates. Studies by us and others revealed that sucralose exacerbated gut damage and inflammation in animal models for inflammatory bowel disease (IBD), including those for both ulcerative colitis, and Crohn's disease. Our study demonstrated that sucralose greatly aggravated dextran sulfate sodium (DSS)-induced colitis along with causing changes in gut microbiota, the gut barrier and impaired inactivation of digestive proteases mediated by deconjugated bilirubin. It is welldocumented that IBD greatly increases the risk of colorectal cancer (CRC), the globally third-most-common cancer, which, like IBD, has a high rate in the developed countries. Azoxymethane (AOM)/DSS has been the most commonly used animal model for CRC. In this study, we further explored the effect of sucralose on tumorigenesis and the possible mechanism involved using the AOM/DSS mouse model. First, 1.5 mg/ml sucralose was included in the drinking water for 6 weeks to reach a relatively stable phase of impact on gut microbiota. Then, 10 mg/kg AOM was administered through intraperitoneal injection. Seven days later, 2.5% DSS was put in the drinking water for 5 days, followed by 2 weeks without DSS. The 5 days of DSS was then repeated, and the mice were sacrificed 6 weeks after AOM injection. The results showed that sucralose caused significant increases in the number and size of AOM/DSS-induced colorectal tumors along with changes in other parameters such as body and spleen weight, pathological scores, mortality, fecal β-glucuronidase and digestive proteases, gut barrier molecules, gut microbiota, inflammatory cytokines and pathways (TNFα, IL-1β, IL-6, IL-10, and TLR4/Myd88/NF-κB signaling), and STAT3/VEGF tumor-associated signaling pathway molecules. These results suggest that sucralose may increase tumorigenesis along with dysbiosis of gut microbiota, impaired inactivation of digestive protease, damage to the gut barrier, and exacerbated inflammation.

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“…In addition, evidence from experimental studies in vitro and in vivo strongly supports the data obtained in human studies. A study on the treatment of experimental colitis in a rat model showed that UCB treatment reduced the in ammatory response induced by trinitrobenzene sulfonic acid and had a strong anti-in ammatory effect [34].…”

Section: Discussionmentioning

confidence: 99%

Association of total bilirubin and indirect bilirubin content with metabolic syndrome among Kazakhs in Xinjiang

Hao

¹

,

Guo

²

,

Ma

³

et al. 2020

Preprint

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Background: Some studies have shown that a high level of bilirubin is a protective factor against metabolic syndrome (MS), while a high level of transaminase is a risk factor for MS. However, the existing results are inconsistent and few cohort studies have been published. Methods: Using an ambispective cohort study, 565 Kazakhs from Xinjiang, China were selected as the study subjects. The baseline serum bilirubin and transaminase levels of the subjects were divided into quartiles and the relationship between these values and the incidence of MS was analyzed. The definition of MS was based on the Joint Interim Statement (JIS) diagnostic criteria. Results: The average follow-up time for the subjects was 5.72 years. The cumulative incidence of MS was 36.11% (204 of the 565 subjects), and the incidence density was 63.10/1000 person-years. Multivariate Cox regression analysis showed that the levels of total bilirubin (TBIL) and indirect bilirubin (IBIL) were negatively correlated with the occurrence of MS, Compared to the lowest quartile level (Q1), the hazard ratios of MS the TBIL levels at the Q2-Q4 quartiles were: 0.47 (0.31-0.71), 0.53 (0.35-0.79), and 0.48 (0.32-0.72), respectively, while IBIL levels at the Q2-Q4 quartiles showed an MS hazard ratio of 0.48 (0.32-0.72), 0.54(0.36-0.81), and 0.52 (0.35-0.77), respectively, all at a 95% confidence level. However, no relationship was found between transaminase levels and the incidence of MS. Conclusion: Serum TBIL and IBIL levels were negatively correlated with the incidence of MS in a Kazakh population in China.

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Unconjugated bilirubin ameliorates the inflammation and digestive protease increase in TNBS-induced colitis

Cited by 17 publications

References 35 publications

Association of total bilirubin and indirect bilirubin content with metabolic syndrome among Kazakhs in Xinjiang

Association of total bilirubin and indirect bilirubin content with metabolic syndrome among Kazakhs in Xinjiang

Sucralose Promotes Colitis-Associated Colorectal Cancer Risk in a Murine Model Along With Changes in Microbiota

Association of total bilirubin and indirect bilirubin content with metabolic syndrome among Kazakhs in Xinjiang

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