Uncoating of tobacco mosaic virus ribonuleic acid after inoculation of tobacco leaves

Shaw, J. G.

doi:10.1016/0042-6822(70)90236-9

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…Estimates of the time required before uncoating was initiated ranged from a few minutes to a few hours, but in these types of experiments the lengths of the uncoated RNA molecules could not be determined since they had been destroyed. In attempts to obtain such information, density-gradient centrifugation analyses of extracts of leaves inoculated with radioactive TMV particles were performed (Shaw 1967(Shaw , 1969(Shaw , 1970Hirashima & Hirai 1969b). In these experiments, it was shown that, a few minutes after inoculation, some coat-protein subunits had been removed from the particles and uncoated viral RNA, a small proportion of it apparently of full length, could be detected.…”

Section: Tmvand Early Events In Virus Infection J G Shaw 605mentioning

confidence: 99%

Tobacco mosaic virus and the study of early events in virus infections

Shaw

1999

Phil. Trans. R. Soc. Lond. B

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In order to establish infections, viruses must be delivered to the cells of potential hosts and must then engage in activities that enable their genomes to be expressed and replicated. With most viruses, the events that precede the onset of production of progeny virus particles are referred to as the early events and, in the case of positive-strand RNA viruses, they include the initial interaction with and entry of host cells and the release (uncoating) of the genome from the virus particles. Though the early events remain one of the more poorly understood areas of plant virology, the virus with which most of the relevant research has been performed is tobacco mosaic virus (TMV). In spite of this e¡ort, there remains much uncertainty about the form or constituent of the virus that actually enters the initially invaded cell in a plant and about the mechanism(s) that trigger the subsequent uncoating (virion disassembly) reactions. A variety of approaches have been used in attempts to determine the fate of TMV particles that are involved in the establishment of an infection and these are brie£y described in this review. In some recent work, it has been proposed that the uncoating process involves the bidirectional release of coat protein subunits from the viral RNA and that these activities may be mediated by cotranslational and coreplicational disassembly mechanisms.

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Section: Tmvand Early Events In Virus Infection J G Shaw 605mentioning

confidence: 99%

Tobacco mosaic virus and the study of early events in virus infections

Shaw

1999

Phil. Trans. R. Soc. Lond. B

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“…The frozen samples of the epidermis and the stripped leaf whidi had been obtained at different times after inoculation were individually ground with a mortar and pestle in 2 ml of cold GPS huffer (pH 9.5) containing 0.1 M glycine, 0.05 M K«HPO^, and 0.3 M NaCl. At this time, one drop of octanol was also added (SHAW 1970). The homogenates were centrifuged at 15000 X g for 30 min.…”

Section: Administration Of ^*P Into the Inoculated Leavesmentioning

confidence: 99%