Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production

Yusoff, Mohd Zulkhairi Mohd; Maeda, Takeyasu; Sánchez-Torres, Viviana; Ogawa, Hiroaki; Shirai, Yoshihito; Hassan, Mohd Ali; Wood, Thomas K.

doi:10.1016/j.ijhydene.2012.08.115

Cited by 30 publications

(11 citation statements)

References 57 publications

(98 reference statements)

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“…The concentration of lactic acid was quantified because it was one of the main compounds produced by probiotic strains in this study. After 24 h incubation following the addition of probiotic cells, the spent supernatant was collected and analyzed for its lactic acid concentration by high performance liquid chromatography (HPLC, Shimadzu LC-10AD) [ 34 ]). The range of lactic acid concentrations produced was used as a reference to determine the effect of exogenously added lactic acid on biofilm degradation.…”

Section: Methodsmentioning

confidence: 99%

Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.

et al. 2016

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The biofilm degradation of Aggregatibacter actinomycetemcomitans is essential as a complete periodontal disease therapy, and here we show the effects of potential probiotic bacteria such as Lactobacillus spp. for the biofilm of several serotypes of A. actinomycetemcomitans strains. Eight of the 13 species showed the competent biofilm degradation of ≥ 90% reduction in biofilm values in A. actinomycetemcomitans Y4 (serotype b) as well as four of the seven species for the biofilm of A. actinomycetemcomitans OMZ 534 (serotype e). In contrast, the probiotic bacteria did not have a big impact for the degradation of A. actinomycetemcomitans SUNY 75 (serotype a) biofilm. The dispersed A. actinomycetemcomitans Y4 cells through the biofilm detachment were still viable and plausible factors for the biofilm degradation were not due to the lactic acid and low pH conditions. The three enzymes, protease, lipase, and amylase may be responsible for the biofilm degradation; in particular, lipase was the most effective enzyme for the biofilm degradation of A. actinomycetemcomitans Y4 along with the protease activity which should be also important for the other serotypes. Remarkable lipase enzyme activities were detected from some of the potential probiotics and a supporting result using a lipase inhibitor presented corroborating evidence that lipase activity is one of the contributing factors for biofilm degradation outside of the protease which is also another possible factor for the biofilm of the other serotype of A. actinomycetemcomitans strains. On the other hand, the biofilm of A. actinomycetemcomitans SUNY 75 (serotype a) was not powerfully degraded by the lipase enzyme because the lipase inhibitor was slightly functional for only two of potential probiotics.

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Section: Methodsmentioning

confidence: 99%

Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.

et al. 2016

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“…The phosphorylation of Akt can induce the translocation of glucose from the cytoplasm to the plasma membrane through glucose transporter 4 (Patki et al, ). Glucose is converted to pyruvate and NADPH through the glycolysis pathways and then converted to acetyl coenzyme A via the pyruvate dehydrogenase complex, which is involved in fatty acid biosynthesis (Maerker, Rohde, Brakhage, & Brock, ; Yusoff et al, ). However, there is limited literature concerning how dietary Met affects the expression of genes involved in glucose metabolism, and whether Met regulates gene expression via the IGF‐I/PI3K/Akt pathway in fish.…”

Section: Introductionmentioning

confidence: 99%

Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Chi

Zhu

et al. 2019

Aquacult Nutr

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Juvenile cobia (Rachycentron canadum), a carnivorous fish, was chosen to examine the effects of methionine (Met) supplementation of low fishmeal diets on growth and the expression of key genes related to hepatic lipogenesis and glucose metabolism. Seven isonitrogenous and isolipidic diets were formulated to contain 7.2, 9.0, 10.0, 12.4, 14.1, 16.3 and 18.6 g/kg dietary Met levels. Healthy cobia (initial average weight ± SE = 9.79 ± 0.04 g) were randomly assigned into seven groups in triplicate with 40 individuals per sea cage for 8 weeks. The results showed that the weight gain rate (WGR) of fish increased as the dietary Met was increased up to 12.4 g/kg and then decreased. The expression of insulin‐like growth factor‐I (IGF‐I) and target of rapamycin (TOR) genes was inhibited by the Met‐deficient diets. The mRNA levels of fatty acid synthase (FAS) and sterol regulatory element‐binding protein‐1 (SREBP1) genes increased with increasing dietary Met until 12.4 g/kg after which the former remained constant, whereas the latter decreased from 12.4 to 18.6 g/kg Met. The mRNA levels of pyruvate kinase (PK) increased as the dietary Met levels increased, reaching a peak at 12.4 g/kg, and then decreased with further increases in Met. However, the mRNA levels of phosphoenolpyruvate carboxykinase (PEPCK) decreased as the dietary Met was increased up to 12.4 g/kg and then remained constant from 12.4 to 18.6 g/kg Met. In conclusion, based on the straight broken‐line analysis of WGR against dietary Met levels, the optimal dietary Met requirement for juvenile cobia was estimated to be 12.4 g/kg (26.9 g/kg dietary protein). A diet with the optimal level of Met induces fish growth and is associated with increased IGF‐I and TOR.

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“…One unit of protease activity was defined as the quantity of tyrosine (mmol) produced from casein per minute by 1 mg of enzyme. Organic acids were analysed using high-performance liquid chromatography (Shimadzu LC-10AD) as described previously (Mohd Yusoff et al 2012). Ammonia concentration in WSS supernatant was measured using ammonia assay kit (Wako, Japan) (Inokuma et al 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Total ribonucleic acid (RNA) was extracted using the RNeasy kit (Qiagen, Valencia, CA) as described previously (Mohd Yusoff et al 2012). The complementary deoxyribonucleic acid (cDNA) was synthesized using the PrimeScript RT Reagent Kits (TAKARA Bio Inc., Shiga, Japan) as described previously (Mustapha et al 2016).…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Effect of Aso limonite on anaerobic digestion of waste sewage sludge

2020

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The effect of Aso volcanic limonite was explored in anaerobic digestion using waste sewage sludge (WSS). In this study, methane and hydrogen sulfide were remarkably inhibited when Aso limonite was mixed with WSS as well as a significant reduction of ammonia. Although pH was lowered after adding Aso limonite, methane was still inhibited in neutralized pH condition at 7.0. Hydrolysis stage was not influenced by Aso limonite as supported by the result that a high protease activity was still detected in the presence of the material. However, acidogenesis stage was affected by Aso limonite as indicated by the different productions of organic acids. Acetic acid, was accumulated in the presence of Aso limonite due to the inhibition of methane production, except in the highest concentration of Aso limonite which the production of acetate may be inhibited. Besides, the production of propionate and butyrate reduced in accordance to the increased concentration of Aso limonite. In addition, Archaeal activity (methanogens) in WSS with Aso limonite was low in agreement with the low methane production. Thus, these results indicate that Aso limonite influences the acidogenesis and methanogenesis processes, by which the productions of methane and ammonia were inhibited. On the other hand, in the contactless of Aso limonite during the anaerobic digestion of WSS (Aso limonite was placed in the area of headspace in the vial), Aso limonite had the adsorptive ability for hydrogen sulfide from WSS, but not for methane. This contactless system of Aso limonite may be a practical means to remove hydrogen sulfide without inhibiting methane production as an important bioenergy source. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production

Cited by 30 publications

References 57 publications

Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.

Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.

Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Effect of Aso limonite on anaerobic digestion of waste sewage sludge

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