Unchanged density of 5-HT1A autoreceptors on the plasma membrane of nucleus raphe dorsalis neurons in rats chronically treated with fluoxetine

Riad, Mustapha; Rbah, Latifa; Verdurand, Mathieu; Aznavour, Nicolas; Zimmer, Luc; Descarries, Laurent

doi:10.1016/j.neuroscience.2007.11.024

Cited by 29 publications

(24 citation statements)

References 57 publications

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“…The positive correlation in raphe is not unexpected because both markers exhibit proportionality to the number of serotonergic neurons. Based on animal studies (Le Poul et al, 1995;Cahir et al, 2007;Riad et al, 2008), however, the anticipation would be an inverted U-shaped relationship between 5-HT 1A receptor and SERT binding in hippocampus and frontal cortex, because 5-HT 1A receptor levels seem to be relatively resistant to changes in cerebral 5-HT levels. The authors did not attempt to model an inverted U-formed relationship, and the relatively low sample size may also have been prohibitive for a more extensive analysis.…”

Section: Discussionmentioning

confidence: 99%

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

Erritzoe¹,

Holst²,

Frøkjær³

et al. 2010

J. Neurosci.

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Section: Discussionmentioning

confidence: 99%

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

Erritzoe¹,

Holst²,

Frøkjær³

et al. 2010

J. Neurosci.

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“…The subcellular localization of 5-HT 1A R was then examined after three weeks of treatment with fluoxetine [36]. The 5-HT 1A autoR are known to be desensitized under these conditions [17,43,47,48], but were unexpectedly found in normal density on the plasma membrane of DRN neurons (figure 2a-c).…”

Section: -Ht 1a Receptors and The Mode Of Action Of Selective Serotomentioning

confidence: 99%

Effects of the antidepressant fluoxetine on the subcellular localization of 5-HT _1A receptors and SERT

Descarries

Riad

2012

Phil. Trans. R. Soc. B

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Serotonin (5-HT) 5-HT 1A autoreceptors (5-HT 1A autoR) and the plasmalemmal 5-HT transporter (SERT) are key elements in the regulation of central 5-HT function and its responsiveness to antidepressant drugs. Previous immuno-electron microscopic studies in rats have demonstrated an internalization of 5-HT 1A autoR upon acute administration of the selective agonist 8-OH-DPAT or the selective serotonin reuptake inhibitor antidepressant fluoxetine. Interestingly, it was subsequently shown in cats as well as in humans that this internalization is detectable by positron emission tomography (PET) imaging with the 5-HT 1A radioligand [ 18 F]MPPF. Further immunocytochemical studies also revealed that, after chronic fluoxetine treatment, the 5-HT 1A autoR, although present in normal density on the plasma membrane of 5-HT cell bodies and dendrites, do not internalize when challenged with 8-OH-DPAT. Resensitization requires several weeks after discontinuation of the chronic fluoxetine treatment. In contrast, the SERT internalizes in both the cell bodies and axon terminals of 5-HT neurons after chronic but not acute fluoxetine treatment. Moreover, the total amount of SERT immunoreactivity is then reduced, suggesting that SERT is not only internalized, but also degraded in the course of the treatment. Ongoing and future investigations prompted by these finding are briefly outlined by way of conclusion.

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“…The laser guidance system at the front of the gantry was used to centre the brain in the FOV. A 10-min transmission scan was acquired using a rotating 57 Co point source, followed by a bolus injection of 7.4-11.1 MBq of [ 18 F]MPPF in the tail vein. Radioactivity was then measured in 27 sequential time frames of increasing duration (8×30, 6×60, 5×120 and 8×300 s) for a total duration of 60 min.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

MicroPET imaging of 5-HT1A receptors in rat brain: a test–retest [18F]MPPF study

Aznavour

Benkelfat

Gravel

et al. 2008

Eur J Nucl Med Mol Imaging

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Purpose Earlier studies have shown that positron emission tomography (PET) imaging with the radioligand [ 18 F]MPPF allows for measuring the binding potential of serotonin 5-hydroxytryptamine 1A (5-HT 1A ) receptors in different regions of animal and human brain, including that of 5-HT 1A autoreceptors in the raphe nuclei. In the present study, we sought to determine if such data could be obtained in rat, with a microPET (R4, Concorde Microsystems). Methods Scans from isoflurane-anaesthetised rats (n=18, including six test-retest) were co-registered with magnetic resonance imaging data, and binding potential, blood to plasma ratio and radiotracer efflux were estimated according to a simplified reference tissue model. ResultsValues of binding potential for hippocampus (1.2), entorhinal cortex (1.1), septum (1.1), medial prefrontal cortex (1.0), amygdala (0.8), raphe nuclei (0.6), paraventricular hypothalamic nucleus (0.5) and raphe obscurus (0.5) were comparable to those previously measured with PET in cats, non-human primates or humans. Test-retest variability was in the order of 10% in the larger brain regions (hippocampus, medial prefrontal and entorhinal cortex) and less than 20% in small nuclei such as the septum and the paraventricular hypothalamic, basolateral amygdaloid and raphe nuclei. Conclusions MicroPET brain imaging of 5-HT 1A receptors with [18 F]MPPF thus represents a promising avenue for investigating 5-HT 1A receptor function in rat.

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Unchanged density of 5-HT1A autoreceptors on the plasma membrane of nucleus raphe dorsalis neurons in rats chronically treated with fluoxetine

Cited by 29 publications

References 57 publications

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

Effects of the antidepressant fluoxetine on the subcellular localization of 5-HT _1A receptors and SERT

MicroPET imaging of 5-HT1A receptors in rat brain: a test–retest [18F]MPPF study

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Unchanged density of 5-HT1A autoreceptors on the plasma membrane of nucleus raphe dorsalis neurons in rats chronically treated with fluoxetine

Cited by 29 publications

References 57 publications

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

Effects of the antidepressant fluoxetine on the subcellular localization of 5-HT 1A receptors and SERT

MicroPET imaging of 5-HT1A receptors in rat brain: a test–retest [18F]MPPF study

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A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

A Nonlinear Relationship between Cerebral Serotonin Transporter and 5-HT_2AReceptor Binding: AnIn VivoMolecular Imaging Study in Humans

Effects of the antidepressant fluoxetine on the subcellular localization of 5-HT _1A receptors and SERT