Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids

Abstract

This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated.

DNA nanostructure decoration: a how-to tutorial

“…This is true whether that band was separated by electrophoretic mobility, sedimentation rate, or size exclusion. Other sources of uncertainty include the linearity of the camera or photodetector, and the subtraction of the background signal [115].…”

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

“…Isothermal amplification is a simplified and faster alternative to PCR, as it does not require denaturation, annealing, and extension steps. The method of detection of the isothermal temperature can be done using turbidimetry [161], fluorescence detection [162][163][164], gel electrophoresis [165][166][167][168], and visual color change chromogenesis [169][170][171][172].…”