When the book Wild mammals of Myanmar (Tun Yin 1993) was published, a total of 252 mammal species were reported. Since that publication, numerous new species have been described, taxonomic revisions published, and additional distribution records produced that have expanded Myanmar’s mammals considerably. Therefore, we aimed to provide an updated checklist of the mammals of Myanmar, which includes the scientific and common names, global and Myanmar distributions, remarks on taxonomy, and conservation information including the IUCN Red List status and legal protection status under The Conservation of Biodiversity and Protected Areas Law 2018 of Myanmar. Our new checklist includes 365 (33 marine and 332 terrestrial) mammal species belonging to 13 orders and 49 families. We also confirmed that Myanmar is home to at least nine endemic mammal species. Our results highlight the areas that require further exploration to gain a more comprehensive understanding of Myanmar’s diverse fauna.