Uncertainties in diagnosis, treatment and prevention of trichinellosis

Shimoni, Zvi; Froom, Paul

doi:10.1586/14787210.2015.1075394

Cited by 22 publications

(25 citation statements)

References 44 publications

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“…Currently, the clinical diagnosis of trichinellosis is very difficult because most Trichinella infections are asymptomatic or with non-specific clinical manifestations (Gottstein et al, 2009; Shimoni and Froom, 2015). Microscopic examination and serological assays are used to diagnosis of Trichinella infection in domestic or wild boars (Gottstein et al, 2009; Cuttell et al, 2012; Fu et al, 2013; Lin et al, 2013; Shimoni and Froom, 2015; Sun et al, 2015). Microscopic examinations are routinely used for the detection of Trichinella larvae in muscle tissues at slaughtering.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

Wang

Zhang

et al. 2019

Front. Cell. Infect. Microbiol.

112

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Trichinella spp., are amongst the most widespread parasitic nematodes, primarily live in the muscles of a wide range of vertebrate animals and humans. Human infection occurs by ingestion of raw or undercooked meat containing Trichinella larvae. Accurate diagnosis of Trichinella spp. infection in domestic animals is crucial for the effective prevention and control of human trichinellosis. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral flow strip (LF-RPA) to detect Trichinella spp. infection. The LF-RPA assay targets Trichinella spp. mitochondrial small-subunit ribosomal RNA (rrnS) gene and can detect as low as 100 fg DNA of Trichinella strains, which was approximately 10 times more sensitive than a conventional PCR assay. The LF-RPA assay can be performed within 10–25 min, at a wide range of temperatures (25–45°C) and showed no cross-reactivity with DNA of other parasites and related host species of Trichinella. The performance of the LF-RPA assay in the presence of high concentration of PCR inhibitor was better than that of a conventional PCR assay. Results obtained by LF-RPA assay for the detection of experimentally infected mice were comparable to the results obtained by using a conventional PCR, achieving 100% specificity and high sensitivity. These results present the developed LF-RPA assay as a new simple, specific, sensitive, rapid and convenient method for the detection of Trichinella infection in domestic animals.

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Section: Introductionmentioning

confidence: 99%

Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

Wang

Zhang

et al. 2019

Front. Cell. Infect. Microbiol.

112

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“…Although these drugs are described as relatively safe, they can induce bone marrow suppression. In addition, they cannot be used in pregnant women due to their teratogenic effects (Shimoni and Froom, 2015 ). Despite the control measures against trichinellosis are mainly based in controlled management of domestic pigs (Gamble et al, 2019 ) and “post-harvest measures,” including testing and processing methods, as well as consumer education (Noeckler et al, 2019 ), vaccination of pigs represents an alternative approach to trichinellosis control, especially for those pigs feeding under backyard or free-ranging conditions (Zhang N. et al, 2018 ).…”

Section: Veterinary Vaccine Development Against Fbds Caused By Parasimentioning

confidence: 99%

Use of Veterinary Vaccines for Livestock as a Strategy to Control Foodborne Parasitic Diseases

Sander

López

Morales

et al. 2020

Front. Cell. Infect. Microbiol.

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Foodborne diseases (FBDs) are a major concern worldwide since they are associated with high mortality and morbidity in the human population. Among the causative agents of FBDs, Taenia solium, Echinococcus granulosus, Toxoplasma gondii, Cryptosporidium spp., and Trichinella spiralis are listed in the top global risk ranking of foodborne parasites. One common feature between them is that they affect domestic livestock, encompassing an enormous risk to global food production and human health from farm to fork, infecting animals, and people either directly or indirectly. Several approaches have been employed to control FBDs caused by parasites, including veterinary vaccines for livestock. Veterinary vaccines against foodborne parasites not only improve the animal health by controlling animal infections but also contribute to increase public health by controlling an important source of FBDs. In the present review, we discuss the advances in the development of veterinary vaccines for domestic livestock as a strategy to control foodborne parasitic diseases.

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“…Besides, traditional anthelminthic drugs are active against enteric stages of T. spiralis, but currently no anthelminthic drug has proven to be effective against the parasite systemic stages [7,8]. Furthermore, serious side effects including bone marrow suppression and teratogenic effects are observed [7,8].…”

Section: Natural Remedies In the Fight Against Parasitesmentioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

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Uncertainties in diagnosis, treatment and prevention of trichinellosis

Cited by 22 publications

References 44 publications

Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay

Use of Veterinary Vaccines for Livestock as a Strategy to Control Foodborne Parasitic Diseases

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

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