UN MÉTODO PRECISO PARA MEDIR SEVERIDAD DE ROYA DE LA HOJA (Puccinia triticina Eriksson) EN TRIGO

Sauceda-Acosta, Carlos P.; Lugo-García, Gabriel Antonio; Villaseñor-Mir, Héctor E.; Ruvalcaba, Leopoldo Partida; Reyes-Olivas, Álvaro

doi:10.35196/rfm.2015.4.427

Cited by 5 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Inoculated leaves were pressed in a botanical press for 15 min and after, digitalized in a scanner (HP Officejet Pro 6830) at 300 dpi resolution. Disease severity was evaluated using digital images and the affected area was calculated by measuring the lesion area (mm 2 ) concerning the size of the leaf by digital image analysis, with ImageJ Software (version 1.48r; NIH, Bethesda, MD, USA) [ 56 ]. The severity data were analyzed by nonparametric ANOVA with Kruskal–Wallis test using InfoStat ® 2017 [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

et al. 2020

Self Cite

View full text Add to dashboard Cite

Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 Neopestalotiopsis isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four Neopestalotiopsis clades from this study and induce lesions on mango leaves. This is the first report of species of Neopestalotiopsis causing mango grey leaf spot disease in Mexico.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…underestimation that frequently occurs when a pictorial diagram is used. A similar method of digital image analysis was used satisfactorily by Sauceda- Acosta et al (2015) to evaluate the severity of Puccinia triticina in wheat through Image J 1.0 software for Windows ® .…”

Section: Conidial Concentration (Conidia/ml)mentioning

confidence: 99%

An alternative inoculation technique of Colletotrichum gloeosporioides on mango for early anthracnose tolerance screening

et al. 2018

View full text Add to dashboard Cite

The importance of having a technique that allows an efficient expression of symptoms of anthracnose is based on the early differentiation of cultivars and the optimization of genetic, material and financial resources. The objective of this research was to generate an alternative inoculation technique for Colletotrichum gloeosporioides on mango for early anthracnose tolerance screening. On this technique was optimized some of the most relevant components such as isolate virulence, conidial density, the inoculum deposition on leaves and using of surfactants. The study was carried in Iguala, Mexico, during the production cycles 2015-2016. C. gloeosporioides was biologically and culturally characterized. Gro and Sin monosporic strains were isolated from leaves, flowers, fruits and branches with anthracnose symptoms from commercial mango orchards located in Guerrero and Sinaloa states, Mexico. These strains show mycelial growth at 2.2 and 2.1 cm of diameter per day, spore density of 4.3x106, 3.9x106 conidia/ml, germination of 27 and 26%, virulence, with incubation period 4.5 and 4.1 days after inoculation, incidence of 90 and 92% and severity of 3.2 and 3.5 cm of diameter. The highest incidence and severity values with the lowest incubation period, was obtained using Gro isolate (1x105 conidia/ml) and polyoxyethylene-20-sorbitan monolaurate as spreader-sticker inoculated on the abaxial surface on detached young leaves, 15-20 days old, with a soft brush and incubated under dark condition. This inoculation technique allowed the optimal expression of C. gloeosporioides virulence in mango leaves and could be incorporated as a tool in the early differentiation of tolerance and susceptibility among cultivars.

show abstract

“…Entre las técnicas de cuantificación de la severidad están las medidas relacionadas, la estimación visual (Cowling y Horsfall, 1978) y el uso de métodos electrónicos (Guzmán et al, 2003). Particularmente para el caso de las royas el método visual es el más utilizado para evaluar la severidad de la enfermedad, debido a su sencillez, rapidez y bajo costo, con el uso de escalas o claves pictográficas que muestran el aumento progresivo de las lesiones; estas escalas diagramáticas, muestran series o partes de plantas con diferentes niveles de gravedad de los síntomas (James, 1971;Navarro y Arauz, 1999;Sauceda-Acosta et al, 2015).…”

Section: Introductionunclassified

Comparación de métodos para cuantificar severidad de la roya del cafeto en diferentes tamaños de hojas

et al. 2022

View full text Add to dashboard Cite

Coffee rust is the most important disease of the crop, the severity of leaf damage caused by the pathogen causes leaf abscission and significantly affects the reduction of yields. This investigation was carried out with the objective of comparing the visual method with a digital one, in the quantification of the percentage of severity of the rust in different sizes of leaves. The quantifications of the disease were carried out at the University Clinic for Agricultural Diagnostics at Oriente University. To carry out the experiment, 360 leaves injured with rust were collected from Catuaí Rojo plantations and divided into three groups, categorized according to leaf area into large, medium and small, each group was represented by 120 leaves, initially evaluated by the visual method by two observers with the help of the diagrammatic scale and later with the digital method by analyzing images with the ImageJ® program. The randomized block design was used with a 2x3 factorial arrangement with ten repetitions per experimental unit. The data were processed by analysis of variance. It was found that the severity percentage did not present statistical differences for the digital methodology when the size of the sheets was changed; while, for the visual methodology, therewas an influence of the size of the leaves, observing that the estimated severity increased as the size of the leaves decreased. The digital method is a reliable alternative for the quantification of the severity of attack by coffee rust.

show abstract

UN MÉTODO PRECISO PARA MEDIR SEVERIDAD DE ROYA DE LA HOJA (Puccinia triticina Eriksson) EN TRIGO

Cited by 5 publications

References 15 publications

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

An alternative inoculation technique of Colletotrichum gloeosporioides on mango for early anthracnose tolerance screening

Comparación de métodos para cuantificar severidad de la roya del cafeto en diferentes tamaños de hojas

Contact Info

Product

Resources

About