Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

Abstract

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data

“… b The DNA G + C content value of Dasania marina was deduced from the genome sequence, the value give in the literature is 37 mol%. The superscript numbers indicate the following used references: 1, Lee et al ( 2007 ); 2, Suarez et al ( 2014 ); 3, Urios et al ( 2011 ); 4, Cheng et al ( 2015 ); 5, Iwaki et al ( 2012 ); 6, Yoon et al ( 2009 ); 7, Chen et al ( 2011 ); 8, Park et al ( 2014 ); 9, Romanenko et al ( 2010 ); 10, Spring et al ( 2013 ); 11, Park et al ( 2012 ); 12, Baba et al ( 2011 ); 13, Zhang et al ( 2012 ); 14, Kämpfer et al ( 2012 ); 15, Jeong et al ( 2013 ); 16, Vashist et al ( 2013 ); 17, Oh et al ( 2010b ); 18, Jang et al ( 2011a ); 19, Su et al ( 2013 ); 20, Lo et al ( 2014 ) . …”

Cocleimonas flava gen. nov., sp. nov., a gammaproteobacterium isolated from sand snail (Umbonium costatum)

“…During a survey of bacteria associated with marine invertebrates inhabiting shallow sediments of the Sea of Japan, we have isolated and characterized several interesting bacterial groups. Of these, two unique pink-redpigmented bacteria isolated from the sand snail Umbonium costatum were recently proposed to represent a novel genus and species, Umboniibacter marinipuniceus, in the class Gammaproteobacteria (Romanenko et al, 2010). Strain KMM 3898 T was simultaneously isolated from the same snail specimen.…”

Umboniibacter roseus sp. nov., isolated from coastal seawater

“…Isoprenoid quinone analyses determined ubiquinone Q-7 as the major compound and Q-8 as the minor compound in strain 14-121-B13 T , similar to U. marinipuniceus JCM 15738 T (Romanenko et al , 2010). The cellular fatty acid profile of strain 14-121-B13 T , together with that of U. marinipuniceus JCM 15738 T , which was also analysed in this study, are shown in .…”

“…The genus Umboniibacter was proposed by Romanenko et al (2010) for a rod-shaped, chemo-organotrophic and strictly aerobic marine bacterium that belonging to the Gammaproteobacteria . At the time of writing, the genus contained only one species, Umboniibacter marinipuniceus (Romanenko et al , 2010), which was isolated from a sand snail. In the course of investigation into the biodiversity of marine bacteria in coastal seawater of the South Sea at Namhae-gun, a number of novel bacterial taxa were isolated.…”