Umbilical cord blood: Morphological composition and functional characteristics

Alexeev, I.; Volynetz, M.; Zamaraeva, N.; Osipova, E. Yu.; Loginov, A.A.; Vladimirskaya, E.; Rumjanzev, A.

doi:10.1007/978-3-642-61122-3_6

Cited by 1 publication

(1 citation statement)

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“…22 The fetus is anatomically and physiologically protected from the systemic circulation of mother by the placental barrier. However, in cases of intercurrent disease during pregnancy and despite the integrity of placental barrier, there are a number of bacterial and viral pathogens capable of crossing it and causing subsequent fetal infections, known as vertically transmitted infections.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterial L-forms are found in cord blood: A potential vertical transmission of BCG from vaccinated mothers

Маркова

Slavchev

Djerov

et al. 2016

Human Vaccines & Immunotherapeutics

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Our previous studies showed that mycobacterial L-forms persist in the blood of BCG vaccinated people and that BCG vaccine is able to produce, under appropriate conditions, filterable, self-replicating L-bodies with virus-like size. Because filterability is one of the characteristics of L-forms, considerable interest has been shown in their capacity to cross the maternal-fetal barrier. The current study demonstrated isolation of mycobacterial L-form cultures from umbilical cord blood of 5 healthy newborns of healthy mothers vaccinated previously with BCG. The isolated cultures showed distinctive growth characteristics of cell wall deficient L-form bacteria. Transmission electron microscopy demonstrated presence of L-bodies with extremely small size of 100 nm and revealed morphological transformations, typical for L-forms. IS6110 Real Time PCR assay confirmed that all L-form isolates were of mycobacterial origin and belonged to Mycobacterium tuberculosis complex which includes vaccinal BCG substrains. In conclusion, we could suggest that reproductive filterable L-bodies of BCG origin are able to fall in blood circulation of the fetus by vertical transmitted pathway and colonize newborns.

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Section: Discussionmentioning

confidence: 99%