Umbelliferone modulates gamma-radiation induced reactive oxygen species generation and subsequent oxidative damage in human blood lymphocytes

Kanimozhi, G.; Prasad, Nagarajan Rajendra; Samivel, Ramachandran; Pugalendi, Kodukkur Viswanathan

doi:10.1016/j.ejphar.2011.09.003

Cited by 44 publications

(25 citation statements)

References 47 publications

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“…UVB-irradiation of cells elicits a complex cellular response via cell surface receptor aggregation [54,55] and upon prolonged exposure; it induces apoptosis in mammalian cells including keratinocytes and lymphocytes. [56] In agreement with this, we observed the protective effect of FA on UVB radiation-induced apoptotic morphological changes. The amount of fragmentation and destruction of irradiated cells were dramatically reduced when the cells were treated with FA [ Figure 8].…”

Section: Discussionsupporting

confidence: 85%

Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts

Ambothi

Prasad

2014

Int J Nutr Pharmacol Neurol Dis

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Aim: Protective effects of ferulic acid (FA) against ultraviolet-B (290-320 nm) radiation induced cellular changes were investigated in human dermal fibroblasts (HDFa). Materials and Methods: HDFa cells pretreated with increasing concentrations of FA (0, 10, 20, 40 μg/ml) for 30 min, were UVB irradiated and different cellular and oxidative end points were analyzed. Results: The percentage of cytotoxicity, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, thiobarbituric acid reactive substances (TBARS) and DNA damage, were significantly increased in 19.8 mJ/cm 2 ultraviolet-B (UVB)-exposed HDFa. Further, exposure to UVB causes significantly decreased antioxidants status in HDFa cells. Treatment of HDFa cells with FA before 30 min of UVB-irradiation significantly restored mitochondrial membrane potential, ROS levels and antioxidant status in HDFa. Further, FA treatment reverted UVB-induced mutagenesis in Ames tester strains and DNA damage in HDFa. Moreover, we noticed increased expression of GADD 45, XRCC1 and HOGG1 in UVB exposed HDFa. Conversely, FA pretreatment significantly attenuated UVB-induced expression of DNA repair genes in HDFa. Conclusion: The present findings indicate that FA act as a sunscreen rather than working at molecular level to offer photoprotection.

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Section: Discussionsupporting

confidence: 85%

Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts

Ambothi

Prasad

2014

Int J Nutr Pharmacol Neurol Dis

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“…However, the most vulnerable populations are either victim of occupational exposure or cancer patients undergoing routine diagnostic or therapeutic radiation treatment. Radiation generates reactive oxygen species (ROS) such as superoxide anion (O À 2 ), hydroxyl radical (•OH) and hydrogen peroxide (H 2 O 2 ) in the cells (Hall, 2000;Kanimozhi, Prasad, Ramachandran, & Pugalendi, 2011). These ROS cause oxidative damage in cellular molecules such as DNA, lipids and proteins (Weiss & Landauer, 2003).…”

Section: Introductionmentioning

confidence: 99%

Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

Bansal

Paul

Kunwar

et al. 2012

Journal of Functional Foods

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“…UMB also reduces the incidence of lymphoedema in humans [14] . Of note, antioxidative, anti-inflammatory and immunomodulatory effects of UMB in both, in vitro and in vivo studies have been reported elsewhere [8,[15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

et al. 2015

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Background/Aims: The aim of this study was to determine the effects of umbelliferone (7-hydroxycoumarin; UMB) on the anticonvulsant potency of four classical antiepileptic drugs (carbamazepine (CBZ), phenytoin (PHT), phenobarbital (PB) and valproate (VPA)) in the mouse maximal electroshock-induced seizure (MES) model. Results: UMB administered systemically intraperitoneally (ip) in a dose of 150 mg/kg significantly elevated the threshold for maximal electroconvulsions (p < 0.05) in mice. Moreover, UMB (150 mg/kg) co-administered with PB and VPA significantly enhanced the anticonvulsant potency of these drugs by reducing their median effective doses (ED₅₀ values) from 35.39 to 21.78 mg/kg (p < 0.01) for PB, and from 281.4 to 215.5 mg/kg (p < 0.01) for VPA. In contrast, UMB (150 mg/kg, ip) had no significant effect on the antiseizure activity of CBZ and PHT in the mouse MES model. Neither total brain PB, nor total brain VPA concentrations were altered after ip administration of UMB, indicating a pharmacodynamic nature of interactions between the tested drugs. Conclusions: The selective potentiation of the anticonvulsant potency of PB and VPA by UMB, and lack of any pharmacokinetic interactions between drugs, make the combinations of UMB with PB or VPA worthy of consideration for epileptic patients who are refractory to standard antiepileptic treatment.

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Umbelliferone modulates gamma-radiation induced reactive oxygen species generation and subsequent oxidative damage in human blood lymphocytes

Cited by 44 publications

References 47 publications

Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts

Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts

Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

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