Umbelliferone induces changes in the structure and pharmacological activities of Bn IV, a phospholipase A2 isoform isolated from Bothrops neuwiedi

Toyama, Daniela de Oliveira; Filho, Eduardo Britto dos Santos Diz; Cavada, Benildo S.; Rocha, B.A.M.; Oliveira, Simone Cristina Buzzo de; Cotrim, Camila A.; Soares, Verônica Cristina Gomes; Delatorre, P.; Marangoni, Sérgio; Toyama, Marcos Hikari

doi:10.1016/j.toxicon.2011.02.024

Cited by 21 publications

(15 citation statements)

References 44 publications

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“…We also found that some pharmacological activities, such as inflammation and myonecrosis, were significantly decreased, but not abolished, after the treatment with 5CQA. This result is in agreement with the results in previously published papers [25, 27, 29, 30], which suggested that there are distinct pharmacological sites in the molecular region near the catalytic site involving the calcium binding loop [26], beta wing [37], and C-terminal [38] of sPLA2.…”

Section: Discussionsupporting

confidence: 93%

“…Toyama et al [30] suggested that enzymatic activity is not required for the antibacterial activity or pharmacological effects induced by the C. d. terrificus PLA2 isoform, and similar results were observed by Diz Filho et al [27] for Crotalus durissus ruruima . Cotrim et al [25] demonstrated the correlation between the enzymatic and antibacterial activities against gram-positive bacteria, and our results strongly reinforce the ideas that the enzymatic activity of sPLA2 is crucial for this activity and that other molecular regions contribute to this effect.…”

Section: Discussionsupporting

confidence: 62%

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Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated fromBaccharis oxyodontaon the Structure and Pharmacological Activities of Secretory Phospholipase A2 fromCrotalus durissus terrificus

Toyama

Ferreira

Romoff

et al. 2014

BioMed Research International

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The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5CQA), isolated from Baccharis oxyodonta, on the structure and pharmacological effect of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus. All in vitro and in vivo experiments were conducted using a purified sPLA2 compared under the same experimental conditions with sPLA2 : 5CQA. 5CQA induced several discrete modifications in the secondary structure and the hydrophobic characteristics of native sPLA2 that induced slight changes in the α-helical content, increase in the random coil structure, and decrease of fluorescence of native sPLA2. Moreover, 5CQA significantly decreased the enzymatic activity and the oedema and myonecrosis induced by native sPLA2. As the catalytic activity of sPLA2 plays an important role in several of its biological and pharmacological properties, antibacterial activity was used to confirm the decrease in its enzymatic activity by 5CQA, which induced massive bacterial cell destruction. We found that 5CQA specifically abolished the enzymatic activity of sPLA2 and induced discrete protein unfolding that mainly involved the pharmacological site of sPLA2. These results showed the potential application of 5CQA in the snake poisoning treatment and modulation of the pathological effect of inflammation induced by secretory PLA2.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 62%

Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated fromBaccharis oxyodontaon the Structure and Pharmacological Activities of Secretory Phospholipase A2 fromCrotalus durissus terrificus

Toyama

Ferreira

Romoff

et al. 2014

BioMed Research International

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“…Evidences show that some of these sPLA2 are involved in the release of arachidonic acid from cellular phospholipids for the biosynthesis of eicosanoids in the inflammatory process (Lambeau, 2008;Lättig et al, 2007;Murakami et al, 1997;Oliveira et al, 2008;Toyama et al, 2011;ValentinLambeau, 2000). Although several experimental data show that sPLA2 may also modulate the activity of cPLA2 and that this modulation may be mediated through specific cell receptors for sPLA2, there is still a discrete mechanism elucidation of the molecular events generated by the interaction of sPLA2 with their receptors (Fonteh et al, 2000;Boilard et al, 2010).…”

Section: Structural Features and Biological Activities Of Spla2mentioning

confidence: 99%

Phospholipases A2 Protein Structure and Natural Products Interactions in Development of New Pharmaceuticals

Toyama¹,

Rodrigues²,

Toyama³

et al. 2012

Protein Structure

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“…Umbelliferone has been shown effect on lipid peroxidation and chemically induced colon carcinogenesis (Muthu et al 2013), antidiabetes (Ramesh and Pugalendi 2006;Kumar et al 2013a), antigenotoxic (Kaur et al 2012;Rezaee et al 2014), antifungal (Bai et al 2012;Islam et al 2012), modify the structure and pharmacological activities of Bn IV (Toyama Dde et al 2011). All these effect of UFD showed the antifungal, antibacterial, anti-inflammatory, antidiabetic, antihyperlipidemic, antioxidant, antigenotoxic activities.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effect of umbelliferon-α-D-glucopyranosyl-(2I→1II)-α-D-glucopyranoside on adjuvant-induced arthritic rats

Kumar

Anwar²,

Verma

et al. 2014

J Food Sci Technol

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The aim and objective of the present investigation was to evaluate the antiarthritic and antioxidant effect of umbelliferon-α-D-glucopyranosyl-(2I→1II)-α-D-glucopyranoside (UFD) in chemically induced arthritic rats. The different doses of the UFD were tested against the turpentine oil (TO), formaldehyde induced acute arthritis and complete fruend's adjuvant (CFA) induced chronic arthritis in Wistar rats. Arthritic assessment and body weight was measured at regular interval till 28 days. On day 28, all the groups animals were anaesthetized, blood were collected from the puncturing the ratro orbital and estimated the hematological parameters. The animals were sacrificed; synovial tissue was extracted and estimated the malonaldehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx) and superoxide dismutase (SOD). The different doses of the UFD showed the protective effect against turpentine oil, formaldehyde induced acute arthritis and CFA induced chronic arthritis at dose dependent manner. Acute model of arthritis such as TOand formaldehyde induced inflammation due to releasing of the inflammatory mediators; significantly inhibited by the UFD at dose dependent manner. CFA induced arthritic rats treated with the different doses of the UFD showed the inhibitory effect on the delayed increase in joint diameter as seen in arthritic control group rats. UFD significantly improved the arthritic index, body weight and confirmed the antiarthritic effect. UFD showed the effect on the hematological parameter such as improved the level of the RBC, Hb and decline the level of the EBC, ESR and confirmed the immune suppressive effect. UFD significantly improved the level of the endogenous antioxidant and confirmed the antioxidant effect. This present investigation suggests that the UFD has prominent antiarthritic impact which can be endorsed to its antiarthritic and antioxidant effects.

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Umbelliferone induces changes in the structure and pharmacological activities of Bn IV, a phospholipase A2 isoform isolated from Bothrops neuwiedi

Cited by 21 publications

References 44 publications

Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated fromBaccharis oxyodontaon the Structure and Pharmacological Activities of Secretory Phospholipase A2 fromCrotalus durissus terrificus

Effect of Chlorogenic Acid (5-Caffeoylquinic Acid) Isolated fromBaccharis oxyodontaon the Structure and Pharmacological Activities of Secretory Phospholipase A2 fromCrotalus durissus terrificus

Phospholipases A2 Protein Structure and Natural Products Interactions in Development of New Pharmaceuticals

Therapeutic effect of umbelliferon-α-D-glucopyranosyl-(2I→1II)-α-D-glucopyranoside on adjuvant-induced arthritic rats

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