UM171 promotes expansion of autologous peripheral blood hematopoietic stem cells from poorly mobilizing lymphoma patients

Wen, Ruiting; Dong, Chao; Xu, Chao; Zhao, Long; Yang, Yang; Zhang, Zhihua; Chen, Yanfu; Duan, Lian; Hu, Chen; Yang, Zhigang; Zhang, Bin

doi:10.1016/j.intimp.2020.106266

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…To identify dominant cell types of each sample library, the scRNA-seq-based cell type recognition tool SingleR (version 1.0.6) was repurposed and applied to the bulk RNA-seq dataset at hand (Aran et al, 2019). Default parameters were used to compute the correlation of each sample against the curated ImmGen reference dataset (Jojic et al, 2013;Shay and Kang, 2013;Aguilar et al, 2020). In particular, subtypes within the broad hematopoietic stem cell compartment were used as reference.…”

Section: Discussionmentioning

confidence: 99%

“…Despite significant increases in functional HSCs, these strategies uniformly required genetic integration, resulting in a risk of leukemic initiation via over-activation of self-renewal programs or blockage of differentiation. To overcome this, numerous groups have explored transient use of extrinsic regulators such as hematopoietic cytokines, growth factors and small molecules to increase HSC self-renewal (Zhang and Lodish, 2009; Fares et al , 2014; Wohrer et al , 2014; Wen et al , 2020). These efforts have culminated in a fully defined culture system that expands mouse HSCs >200-fold over a 28-day period (Wilkinson et al , 2019).…”

Section: Introductionmentioning

confidence: 99%

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A highly efficient reporter system for identifying and characterizing in vitro expanded hematopoietic stem cells

Jlc

Bode

Kuciński

et al. 2021

Preprint

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Hematopoietic stem cells (HSCs) cultured outside the body are the fundamental component of a wide range of cellular and gene therapies. Recent efforts have achieved more than 200-fold expansion of functional HSCs, but their molecular characterization has not been possible due to the substantial majority of cells being non-HSCs and single cell-initiated cultures displaying substantial clone-to-clone variability. Using the Fgd5 reporter mouse in combination with the EPCR surface marker, we report exclusive identification of HSCs from non-HSCs in expansion cultures. Linking single clone functional transplantation data with single clone gene expression profiling, we show that the molecular profile of expanded HSCs is similar to actively cycling fetal liver HSCs and shares a gene expression signature with functional HSCs from all sources, including Prdm16, Fstl1 and Palld. This new tool can now be applied to a wide-range of functional screening and molecular experiments previously not possible due to limited HSC numbers.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A highly efficient reporter system for identifying and characterizing in vitro expanded hematopoietic stem cells

Jlc

Bode

Kuciński

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Despite significant increases in functional HSCs, these strategies uniformly required genetic integration, resulting in a risk of leukemic initiation via over‐activation of self‐renewal programs or blockage of differentiation. To overcome this, numerous groups have explored transient use of extrinsic regulators such as hematopoietic cytokines, growth factors, and small molecules to increase HSC self‐renewal (Zhang & Lodish, 2008 ; Fares et al , 2014 ; Wohrer et al , 2014 ; Wen et al , 2020 ). These efforts have culminated in a fully defined culture system that expands mouse HSCs > 200‐fold over a 28‐day period (Wilkinson et al , 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of in vitro expanded hematopoietic stem cells

et al. 2022

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“…A study explored the culture of hPSC-HPs in HSC expansion conditions. In the presence of THPO, SCF, FLT3L, IL3 and IL-6, the UM171-expanded is a specialized phenotype CD34+CD41aloCD45+ and was enriched in granulocytic progenitors (G-CFCs) [ 62 ]. However, in the cultures with SCF, FLT3L and IL7, UM171 selectively amplified the CD34+CD45+CD7+ phenotype with NK cells [ 60 ].…”

Section: Ex Vivo Expansion Of Hscsmentioning

confidence: 99%

New Insights into Hematopoietic Stem Cell Expansion to Stimulate Repopulation of the Adult Blood System for Transplantation

Xuan

Liu

et al. 2022

Life

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Successful engraftment of hematopoietic stem cells (HSCs) and progenitor cells (HSPCs) may be considered as a basis for the repopulation of the blood cells after transplantation in adults. Therefore, in vivo and ex vivo expansion of HSCs holds great promise for clinical applications. In this review, the mechanisms of HSC expansion will be discussed, considering the previous studies and works of literature. This is aimed to identify the signaling pathways that regulate HSC expansion and improve the application of engraftment in disease management. The following aspects will be included: (i) Stimulation of HSCs growth in vivo through gene regulation and cytokines activation; (ii) direct or indirect induction of HSC expansion by regulating signaling pathways; (iii) addition to assisting cells to help in the proliferation of HSCs; (iv) changing of living environment in the HSCs cultures via adjusting components and forms of cultures; (v) enhancement of HSC expansion by incorporating substances, such as extracellular vesicles (EVs), UM171, among others. In this review, recent new findings that provide us with new insights into HSC expansion methods have been summarized. Furthermore, these findings will also provide more possibilities for the development of some novel strategies for expanding and engrafting HSCs applied for treatments of some hematopoietic disorders.

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UM171 promotes expansion of autologous peripheral blood hematopoietic stem cells from poorly mobilizing lymphoma patients

Cited by 9 publications

References 25 publications

A highly efficient reporter system for identifying and characterizing in vitro expanded hematopoietic stem cells

A highly efficient reporter system for identifying and characterizing in vitro expanded hematopoietic stem cells

Identification and characterization of in vitro expanded hematopoietic stem cells

New Insights into Hematopoietic Stem Cell Expansion to Stimulate Repopulation of the Adult Blood System for Transplantation

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