UM‐SCC‐104: A New human papillomavirus‐16–positive cancer stem cell–containing head and neck squamous cell carcinoma cell line

Abstract

BACKGROUND Few human papillomavirus (HPV)-positive head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established UM-SCC-104, a new HPV(+) HNSCC cell linefrom a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSC). METHODS Tumor cells were tested for biomarker expression by immunohistology and the presence of HPV was assessed by several methods. RESULTS UM-SCC-104 has a unique genotype, contains HPV-16 and expresses E6/E7. Inoculation of (Aldehyde Dehydrogenase) ALDH(+) and ALDH(−) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, while ALDH(−) cells did not produce tumors. CONCLUSIONS UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of EGFR and a functionally significant proportion of ALDH(+) CSC.

Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability

“…UM-SCC-47 (Lansford et al 1999) and UM-SCC-104 (Tang et al 2012) were kindly provided by Thomas Carey, University of Michigan; UPCI:SCC090 (White et al 2007) were from Susanne M. Gollin, University of Pittsburgh; UD-SCC-2 (Ballo et al 1999) were from Henning Bier, University of Dusseldorf; and HMS001 cells were from James Rocco, Harvard Medical School. Please see Table 1 and Supplemental Table 1. CAL 27, D562, SCC-25, SiHa, and CaSki cells were cultured as recommended by ATCC.…”

Primary Chemotherapy and Radiation as a Treatment Strategy for HPV-Positive Oropharyngeal Cancer

“…To date, the major hurdle for HPV/OPSCC screening is the paucity of relevant HPV/ OPSCC cell line models to be used for larger scale drug screening. Currently, there are only 5 HPV/HNC cell lines reported (UD-SCC-2 from hypopharynx, UPCI:SCC90 from the base of tongue, UM-SCC47 from lateral tongue, 93-VU-147T from the floor of the mouth and UM-SCC-104 from recurrent oral cavity [20]; and only one of these was derived from the oropharynx (UPCI:SCC90). Therefore, the development of additional HPV/OPSCC cell models will be of paramount importance to enhance the drug development process for HPV/OPSCC.…”

Human Papillomavirus Detection in Head and Neck Squamous Cell Carcinomas and Its Clinical Implications

“…Some studies have observed high expression of nuclear p53 in some human papillomavirus-containing tumors with wild-type p53 (Hafkamp et al, 2003), and other studies demonstrating low p53 expression (Wilczynski et al, 1998). Mechanism of overexpression of wild-type p53 in the presence of the virus is known (Tang et al, 2011).…”

“…The variability of high risk human papillomavirus containing cell lines enhances our ability to study the role that human papillomavirus plays in head and neck squamous cell carcinoma development and response or resistance to therapy (Tang et al, 2011). Combination of several risk factors could explain this.…”

“…Combination of several risk factors could explain this. A positive tobacco history in patients with human papillomavirus positive tumors may represent a distinct group of head and neck squamous cell carcinoma when all head and neck squamous cell carcinoma are divided by etiologic factors: human papillomavirus negative smokers, human papillomavirus positive never smokers, human papillomavirus positive ever smokers, etc… TPV status likely provided an additive and possibly synergistic effect with others risk factors (Tang et al, 2011).…”