Abstract:Protein transport is an interesting and intrinsic life feature that is highly relevant to physiology and disease in living beings. Herein, inspired by nature, based on the supramolecular host-guest interaction, we have introduced the classical azobenzene light switches and L-phenylalanine derived pillar[6]arene (L-Phe-P6) into the artificial nanochannel to construct light-responsive nanochannels that could regulate protein transport effectively under the control of ultraviolet (UV) and visible (Vis) light. The… Show more
The precise regulation of chiral drug transmembrane transport can be achieved through drug transporters in living organisms. However, implementing this process in vitro is still a formidable challenge due to the complexity of the biological systems that control drug enantiomeric transport. Herein, a facile and feasible strategy is employed to construct chiral L‐tyrosine‐modified nanochannels (L‐Tyr nanochannels) based on polyethylene terephthalate film, which could enhance the chiral recognition of propranolol isomers (R‐/S‐PPL) for transmembrane transport. Moreover, conventional fluorescence spectroscopy, patch‐clamp technology, laser scanning confocal microscopy, and picoammeter technology are employed to evaluate the performance of nanochannels. The results show that the L‐Tyr nanochannel have better chiral selectivity for R‐/S‐PPL compared with the L‐tryptophan (L‐Trp) channel, and the chiral selectivity coefficient is improved by about 4.21‐fold. Finally, a detailed theoretical analysis of the chirality selectivity mechanism is carried out. The findings would not only enrich the basic theory research related to chiral drug transmembrane transport, but also provide a new idea for constructing artificial channels to separate chiral drugs.
The precise regulation of chiral drug transmembrane transport can be achieved through drug transporters in living organisms. However, implementing this process in vitro is still a formidable challenge due to the complexity of the biological systems that control drug enantiomeric transport. Herein, a facile and feasible strategy is employed to construct chiral L‐tyrosine‐modified nanochannels (L‐Tyr nanochannels) based on polyethylene terephthalate film, which could enhance the chiral recognition of propranolol isomers (R‐/S‐PPL) for transmembrane transport. Moreover, conventional fluorescence spectroscopy, patch‐clamp technology, laser scanning confocal microscopy, and picoammeter technology are employed to evaluate the performance of nanochannels. The results show that the L‐Tyr nanochannel have better chiral selectivity for R‐/S‐PPL compared with the L‐tryptophan (L‐Trp) channel, and the chiral selectivity coefficient is improved by about 4.21‐fold. Finally, a detailed theoretical analysis of the chirality selectivity mechanism is carried out. The findings would not only enrich the basic theory research related to chiral drug transmembrane transport, but also provide a new idea for constructing artificial channels to separate chiral drugs.
Biological channels in cell membrane take on a critical significance to the regulation of signal transduction and transmembrane transport. Researchers have been committed to building biomimetic nanochannels to imitate the...
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