Ultraviolet Sensitivity Gene of
            <i>Escherichia coli</i>
            B

Donch, John; Greenberg, Joseph

doi:10.1128/jb.95.5.1555-1559.1968

Cited by 58 publications

(14 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This mutant was Plkc sensitive and also T7 sensitive, The mutation to mucoid (excess capsule formation) can occur at two distinct loci: capR (synonomous with Ion; see references 3,14,19) and capS (21). The capR mutants are also UV sensitive (3,9,14). ES370 was not UV sensitive and assumed to be a capS mutation.…”

Section: Resultsmentioning

confidence: 96%

“…The receptors for phages T3, T4, and T7 are in the lipopolysaccharide layer of the cell wall, and the T2 and T6 receptors are in the lipoprotein (30,31). In E. coli B, which cannot become mucoid (9), no cross-resistance is found among the T2, T6, and the T3, T4, T7 groups. It has been shown in E. coli B that resistance to phages T3, T4, and T7 (B/3, 4, 7) is due to a change in the lipopolysaccharide (32).…”

Section: Resultsmentioning

confidence: 99%

“…DISCUSSION E. coli B, the original host of the T phages and the strain in which most work concerning T phage cross-resistance has been done, is presumably a capR non double mutant. Donch and Greenberg (9) showed that strain B is UV sensi- . These mutants are nonmucoid, form at a frequency of about 10-6, and the B/3, 4, 7 mutants have an altered cell wall lipopolysaccharide, the receptor site of phages T3, T4, and T7 (32).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Radke

Siegel

1971

J Bacteriol

View full text Add to dashboard Cite

A mutant strain of Escherichia coli K-12 was found in which spontaneous mutation to phage T7 resistance occurred at a very low frequency. T7 resistance in the parental strain from which this mutant was derived resulted from a mutation to excess capsular polysaccharide synthesis. non-9, inhibited capsule formation when non-9 mutation was cotransducible with non-9 his Su-l.The mutation preventing T7 resistance, transduced into capsulated strains. The his, the gene order in this region being The T phages of Escherichia coli have in common the ability to adsorb to and replicate in E. coli strains B and K-12. Compared with many E. coli strains found in nature, strains B and K-12 are "rough," i.e., they do not have significant amounts of extracellular polysaccharide capsular material. The T phages adsorb to specific sites on the cell wall (30). Mutants of E. coli K-12 and other strains of E. coli are found that produce very large amounts of capsular material and are mucoid or "smooth" in appearance (16,19,24). Some, but not all of these mutants, are resistant to the T phages and other coliphages because the cell wall phage receptors are covered by the capsule polysaccharide (16, 24).We have found a mutant of E. coli K-12 that mutates at a very low frequency to resistance to phage T7. This work will show that this unusual phenotype is due to a block in capsule formation.MATERIALS AND METHODS Bacterial strains, phages, media, and growth conditions. The bacterial strains used are listed in Table 1. Wild-type T phages were obtained from laboratory stocks and grown and assayed on E. coli B by using procedures described by Adams (1). The methods for P 1 kc growth and transduction were previously described (27).The minimal medium was that of Davis and Mingioli (6) plus I ug of thiamine hydrochloride and 0.5 sg of ferrous sulfate per ml. When necessary, amino acids were used at a final concentration of 50 tig/ml. Nutrient broth contained 0.8% (w/v) nutrient broth powder (Difco), 0.1% yeast extract (Difco), and 0.5% sodium chloride. Solid media (nutrient and minimal agar) were made by adding 20 g of agar (Difco) per liter to the liquid medium. Antibiotic medium no. 3 (Difco) was occasionally used in place of nutrient broth. Bromothymol blue medium, used to score fermentation markers, contained 0.8% Difco nutrient broth powder, 1.0% sugar, 0.006% bromothymol blue, and 2% agar.Overnight, aerated cultures grown at 37 C were used as stationary-phase cultures. Liquid cultures and plates were incubated at 30 C when necessary to increase capsule formation; all other incubations were at 37 C. Genetic procedures. All matings were uninterrupted. Donor and recipient cultures were mixed and incubated for I to 2 hr before plating as described (27). In transductions, a multiplicity of infection of one was used. All recombinants were purified by single-colony isolation on the selective medium.Unselected auxotrophic and fermentation markers were scored by replica plating. The Su-l+ (suppressor containing) and Su-1alleles were scored by crossst...

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Radke

Siegel

1971

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Although the specificity ratios for B1-8 IgM and separately expressed i and X are very similar, lower concentrations of NIP and NP are required for inhibition of separately expressed X and X compared to B1-8 IgM. (23,24), so that the absence of this protease either acting upon p, or being responsible for activating other proteases that act on p, may be the major factor resulting in accumulation in E. coli B.…”

Section: Nucleic Acids Researchmentioning

confidence: 99%

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

et al. 1984

Nucl Acids Res

154

View full text Add to dashboard Cite

Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy chain polypeptides in appropriate host strains. Both proteins were found as insoluble products. Following extraction and purification of the immunoglobulin containing fractions, antigen binding activity was recovered. The activity demonstrates essentially the same properties as the antibody from the hybridoma from which the genes were cloned.

show abstract

“…Although these treatments may rescue a variety of strains [5] they were first demonstrated, and exert their largest effect, on E. coli strain B . This strain carries a mutation, lon [6], conferring a reduced ability to re-initiate cell division following U.V. irradiation, resulting in the formation of filaments and death.…”

Section: Introductionmentioning

confidence: 99%

THE EFFECT OF A uvr MUTATION ON RESCUE OF U.V. IRRADIATED LON STRAINS OF *ESCHERICHIA COLI**

Green¹,

Donch²,

Greenberg³

1969

Photochem & Photobiology

View full text Add to dashboard Cite

Abstract— Treatments such as plating at elavated temperature, plating in the presence of pantoyl lactone, plating on minimal medium, plating on complete medium after intense white illumination, or plating on complete medium after holding in buffer, are known to increase survival of U.V. irradiated Lon strains of Escherichia coli. The effect of these treatments on Hcr Lon+ and Hcr Lon strains was studied. Pantoyl lactone and elavated temperature recovery seem specifically related to the Lon defect. Holding in buffer does not enhance survival in an Hcr strain, and only exerts a small effect in an Hcr Lon strain. White irradiation and minimal medium may greatly enhance recovery of both Hcr and Lon strains. However, an Hcr Lon strain is sensitive to white irradiation itself.

show abstract

Ultraviolet Sensitivity Gene of Escherichia coli B

Cited by 58 publications

References 7 publications

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

THE EFFECT OF A uvr MUTATION ON RESCUE OF U.V. IRRADIATED LON STRAINS OF *ESCHERICHIA COLI**

Contact Info

Product

Resources

About

Ultraviolet Sensitivity Gene of Escherichia coli B

Cited by 58 publications

References 7 publications

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

THE EFFECT OF A uvr MUTATION ON RESCUE OF U.V. IRRADIATED LON STRAINS OF ESCHERICHIA COLI*

Contact Info

Product

Resources

About

THE EFFECT OF A uvr MUTATION ON RESCUE OF U.V. IRRADIATED LON STRAINS OF *ESCHERICHIA COLI**