Ultraviolet reactivation of a bacteriophage containing a single-stranded deoxyribonucleic acid as a genetic element

Ono, Joji; Shimazu, Yoshie

doi:10.1016/0042-6822(66)90036-5

Cited by 48 publications

(8 citation statements)

References 16 publications

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“…An inducible product responsible for such errors could be: (1) a DNA polymerase capable of continuous replication past a pyrimidine dimer, and thus able to rejoin daughter-strand segments by error-prone repair synthesis (9); or (2) a product which participates in the recombinational repair of daughter-strand gaps, causing this process to become error-prone. The occurrence of W-reactivation in single-stranded phages, under conditions which preclude recombinational exchanges (23)(24)(25), argues strongly that an error-prone system of repair replication is among the inducible functions expressed in genetically damaged E. coli cells.…”

Section: Resultsmentioning

confidence: 99%

Thermal Enhancement of Ultraviolet Mutability in a tif-1 uvrA Derivative of Escherichia coli B/r: Evidence That Ultraviolet Mutagenesis Depends upon an Inducible Function

Witkin

1974

Proc. Natl. Acad. Sci. U.S.A.

183

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Inhibition of DNA replication in E. coli results in coordinate expression of a cluster of diverse functions, including prophage induction and filamentous growth, many of which are thermally inducible, without inhibition of DNA synthesis, in strains carrying a mutation in the "tif" locus. It has been proposed that one of the inducible functions in this cluster is responsible for the ultraviolet mutability of E. coli. As a test of this hypothesis, the effect of postirradiation temperature elevation on ultraviolet mutability was compared in strains differing by a tif mutation. In a tif+ strain, raising the temperature to 420 after irradiation did not change the frequency of induced mutations to tryptophan-independence. Similar treatment of a tif-1 strain resulted in as much as a 10-fold increase in the induced mutation yield. Thermal enhancement of ultraviolet mutability in the tif-1 strain was prevented or promoted by agents known to exert parallel effects on thermal induction of lambda prophage in tif lysogens. The results support the hypothesis that ultraviolet mutability in E. coli depends upon an inducible function that is normally expressed only when DNA replication is inhibited.Properties of Escherichia coli strains lacking DNA polymerase I (1, 2) support the hypothesis (3, 4) that a function necessary for ultraviolet (UV) mutagenesis is inducible, and that its induction has the same requirements as a number of other UVinducible functions, exemplified by induction of prophage lambda. These requirements normally include a recA + lex + genotype and conditions under which DNA replication is inhibited while RNA and protein synthesis continue. However, in tif mutant strains of E. coli K12, prophage induction, filamentous growth, W-reactivation, and W-mutagenesis (see Abbreviations) (5) detected? Since UV-induced mutations are believed to originate as errors in the repair of daughter-strand gaps opposite unexcised pyrimidine dimers (9, 10), the postulated inducible function may have no demonstrable effects in unirradiated cells, which contain no such gaps. Irradiated bacteria, therefore, must be used to detect thermally induced UV mutability. However, since all but the lowest doses of UV are themselves efficient inducers of UV-inducible functions, presumably including the one in question, a test for thermal induction of UV mutability must be conducted only with cells exposed to extremely low doses of UV. In uvrA strains, doses below about 5 ergs/mm2 induce very little prophage, (11) and promote very little W-reactivation (3). Irradiation of uvrA tif bacteria with UV doses in this low range, therefore, should introduce some pyrimidine dimers capable of producing daughter-strand gaps (about 6 per erg/mm2 per E. coli genome) without also inducing the capacity to effect UV mutagenesis in more than a very small fraction of the population, thus providing conditions necessary for a biological assay of thermal inducibility of this capacity. The hypothesis that UV mutagenesis depends upon an inducible function gen...

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Section: Resultsmentioning

confidence: 99%

Thermal Enhancement of Ultraviolet Mutability in a tif-1 uvrA Derivative of Escherichia coli B/r: Evidence That Ultraviolet Mutagenesis Depends upon an Inducible Function

Witkin

1974

Proc. Natl. Acad. Sci. U.S.A.

183

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“…The singlestranded DNA phage R fails to be reactivated by the HCR mechanism of E. coli but is competent for W-reactivation (Ono and Shimazu 1966), which further suggests that Wreactivation and host cell reactivation are due to independent mechanisms.…”

Section: *mentioning

confidence: 99%

“…P1 (Kerr and Hart 1973); P2 (Bertani 1960); HPI (Harm and Rupert 1963);and T7 (McKee and Hart 1975); and in several bacteriophage containing single-stranded DNA including S13 (Tessmand and Ozaki 1960); OR (Ono and Shimazu 1966); and 0X174 (Das Gupta and Poddar 1975).…”

Section: *mentioning

confidence: 99%

“…Phage damage by agents other than UVirradiation also show W-reactivation, such as gamma radiation (Bresler et al 1978), nitious acid (Ono and Shimazu 1966), hydroxylamine (Vizdalova 1969), nitrogen mustard (Kerr and Hart 1972), and 5-bromouracil incorporation before UV-irradiation (Kneser et al 1965;Kerr and Hart 1972).…”

Section: *mentioning

confidence: 99%

“…and RNA synthesis is essential for W-reactivation (Ono and Shimazu 1966). Protein synthesis is also necessary for UV-mutagenesis (Witkin 1956) and for UV-induction of prophage X (Tomizawa and Ogawa 1967).…”

Section: Proteinmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Inducible DNA Repair in W-Reactivation and Related Phenomena

Knudson¹

1983

Progress in Molecular and Subcellular Biology

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Effects of Ultraviolet-Light and of Some Major Chemical Carcinogens of E. Coli K12 (λ)

Devoret¹

1977

Research in Photobiology

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Ultraviolet reactivation of a bacteriophage containing a single-stranded deoxyribonucleic acid as a genetic element

Cited by 48 publications

References 16 publications

Thermal Enhancement of Ultraviolet Mutability in a tif-1 uvrA Derivative of Escherichia coli B/r: Evidence That Ultraviolet Mutagenesis Depends upon an Inducible Function

Thermal Enhancement of Ultraviolet Mutability in a tif-1 uvrA Derivative of Escherichia coli B/r: Evidence That Ultraviolet Mutagenesis Depends upon an Inducible Function

The Role of Inducible DNA Repair in W-Reactivation and Related Phenomena

Effects of Ultraviolet-Light and of Some Major Chemical Carcinogens of E. Coli K12 (λ)

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