Ultraviolet radiation significantly enhances the molecular response to dispersant and sweet crude oil exposure in Nematostella vectensis

Tarrant, Ann M.; Payton, Samantha L.; Reitzel, Adam M.; Porter, Danielle T; Jenny, Matthew J.

doi:10.1016/j.marenvres.2018.01.002

Cited by 18 publications

(17 citation statements)

References 90 publications

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“…47 Recently ovoA transcription was also found to be up-regulated in Nematostella vectensis (starlet sea anemone), in response to dispersant and/or sweet crude oil exposure alone or combined with ultraviolet radiation (UV). 48 These data confirm the involvement of this metabolic pathway in the defence mechanisms from environmental factors and highlight the importance of these sulphurcontaining amino acids for UVA protection in area more exposed to radiation such as the poles.…”

Section: Description Of the Mechanistic And Structural Basis For Ovotsupporting

confidence: 71%

On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential

Castellano

Seebeck

2018

Nat. Prod. Rep.

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Covering: up to 2018Ovothiols are sulfur-containing natural products biosynthesized by marine invertebrates, microalgae, and bacteria. These compounds are characterized by unique chemical properties suggestive of numerous cellular functions. For example, ovothiols may be cytoprotectants against oxidative stress, serve as building blocks of more complex structures and may act as molecular messengers for inter- and intracellular signaling. Detailed understanding of ovothiol physiological role in marine organisms may unearth novel concepts in cellular redox biochemistry and highlight the therapeutic potential of this antioxidant. The recent discovery of ovothiol biosynthetic genes has paved the way for a systematic investigation of ovothiol-modulated cellular processes. In this highlight we review the early research on ovothiol and we discuss key questions that may now be addressed using genome-based approaches. This highlight article provides an overview of recent progress towards elucidating the biosynthesis, function and potential application of ovothiols.

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Section: Description Of the Mechanistic And Structural Basis For Ovotsupporting

confidence: 71%

On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential

Castellano

Seebeck

2018

Nat. Prod. Rep.

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“…The biological role of these molecules has often been linked to their peculiar antioxidant properties [26,27] mediating defense from oxidative stress during fertilization and development in the sea urchin [47,48], from environmental stressors in fish eye lenses [49], from macrophage-triggered stress in pathogenic parasites during infection [50] and from light-dependent stress in microalgae [38,39]. In addition, ovothiols have been recently suggested to protect the sea anemone from the stress induced by UV radiation [51] and mussels from environmental pollutants during spawning [52]. However, biological functions not strictly related to oxidative stress have also been discovered.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Light Response of Skeletonema marinoi: Involvement of Ovothiol

et al. 2020

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Diatoms are one of the most widespread groups of microalgae on Earth. They possess extraordinary metabolic capabilities, including a great ability to adapt to different light conditions. Recently, we have discovered that the diatom Skeletonema marinoi produces the natural antioxidant ovothiol B, until then identified only in clams. In this study, we investigated the light-dependent modulation of ovothiol biosynthesis in S. marinoi. Diatoms were exposed to different light conditions, ranging from prolonged darkness to low or high light, also differing in the velocity of intensity increase (sinusoidal versus square-wave distribution). The expression of the gene encoding the key ovothiol biosynthetic enzyme, ovoA, was upregulated by high sinusoidal light mimicking natural conditions. Under this situation higher levels of reactive oxygen species and nitric oxide as well as ovothiol and glutathione increase were detected. No ovoA modulation was observed under prolonged darkness nor low sinusoidal light. Unnatural conditions such as continuous square-wave light induced a very high oxidative stress leading to a drop in cell growth, without enhancing ovoA gene expression. Only one of the inducible forms of nitric oxide synthase, nos2, was upregulated by light with consequent production of NO under sinusoidal light and darkness conditions. Our data suggest that ovothiol biosynthesis is triggered by a combined light stress caused by natural distribution and increased photon flux density, with no influence from the daily light dose. These results open new perspectives for the biotechnological production of ovothiols, which are receiving a great interest for their biological activities in human model systems.

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“…We hypothesized that along oogenesis, there would be an increase in ovoA transcription from the resting to the spawning phase in order to accumulate OSH-A in mature eggs. Oocytes before spawning are involved mainly in reserve building processes for the latter embryo, and gene transcription rhythms analyzed along oogenesis have demonstrated a peak in transcript abundance at the ripe phase [26]. The same has been reported to occur along spermatogenesis [26].…”

Section: Ovoa In M Galloprovincialis and Gene Transcription Levelsmentioning

confidence: 69%

“…Oocytes before spawning are involved mainly in reserve building processes for the latter embryo, and gene transcription rhythms analyzed along oogenesis have demonstrated a peak in transcript abundance at the ripe phase [26]. The same has been reported to occur along spermatogenesis [26]. OSH-A accumulation could be following this increase in transcriptional activity concentration picking just previous to spawning.…”

Section: Ovoa In M Galloprovincialis and Gene Transcription Levelsmentioning

confidence: 72%

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

et al. 2020

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Reactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization.

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Ultraviolet radiation significantly enhances the molecular response to dispersant and sweet crude oil exposure in Nematostella vectensis

Cited by 18 publications

References 90 publications

On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential

On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential

Insights into the Light Response of Skeletonema marinoi: Involvement of Ovothiol

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

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