Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo

Seo, Jin Young; Lee, Seong Hun; Youn, Choon Shik; Choi, Hai Ryung; Rhie, Gi‐eun; Cho, Kwang Hyun; Kim, Kyu Han; Park, Kyung Chan; Eun, Hee Chul; Chung, Jin Ho

doi:10.1046/j.1523-1747.2001.01358.x

Cited by 91 publications

(77 citation statements)

References 21 publications

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“…Moreover, Weiss [55] reported a reduction in elastin production with age or as a result of damage caused by solar radiation. However, the occurrence of elastosis assumes an increase in elastic material in the photoaged skin, as observed in other studies [22,56]. This seemingly paradoxical situation should be analysed by considering the non-functionality of the fibres present in elastosis.…”

Section: Elastin Role On Photoageingmentioning

confidence: 71%

Elastin structure and its involvement in skin photoageing

Weihermann

Lorencini

Brohem

et al. 2016

Intern J of Cosmetic Sci

139

102

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Skin aging is a complex process that may be caused by factors that are intrinsic and extrinsic to the body. Ultraviolet (UV) radiation represents one of the main sources of skin damage over the years and characterizes a process known as photoaging. Among the changes that affect cutaneous tissue with age, the loss of elastic properties caused by changes in elastin production, increased degradation and/or processing produces a substantial impact on tissue esthetics and health. The occurrence of solar elastosis is one of the main markers of cutaneous photoaging and is characterized by disorganized and non-functional deposition of elastic fibers. The occurrence of UV radiation-induced alternative splicing of the elastin gene, which leads to inadequate synthesis of the proteins required for the correct assembly of elastic fibers, is a potential explanation for this phenomenon. Innovative studies have been fundamental for the elucidation of rarely explored photoaging mechanisms and have enabled the identification of effective therapeutic alternatives such as cosmetic products. This review addresses cutaneous photoaging and the changes that affect elastin in this process.R esum e Le vieillissement de la peau est un processus complexe qui peutêtre caus e par des facteurs intrins eques et extrins eques du corps. Les rayons ultraviolets (UV) repr esentent l'une des principales sources de dommages de la peau au fil des ans et caract erise un processus connu sous le nom de photo-vieillissement. Parmi les changements qui affectent le tissu cutan e avec l'âge, la perte des propri et es elastiques caus ees par des changements dans la production d' elastine, augmentation de la d egradation et / ou le traitement, produit un impact important sur l'esth etique et la sant e des tissus. La survenue d'une elastose solaire est l'un des principaux marqueurs de photo-vieillissement cutan e et se caract erise par un d epôt d esordonn e et non-fonctionnel des fibres elastiques. L'apparition d'un epissage alternatif du g ene de l' elastine induit par le rayonnement UV, ce qui conduit a une synth ese insuffisante des prot eines n ecessaires a l'assemblage correct des fibres elastiques, est une explication possible de ce ph enom ene. Des etudes novatrices ont et e fondamentales pour l' elucidation des m ecanismes de photovieillissement rarement explor es et ont permis l'identification d'alternatives th erapeutiques efficaces, tels que les produits cosm etiques. Cette revue porte sur le photo-vieillissement cutan e et les changements qui affectent l' elastine dans ce processus.

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Section: Elastin Role On Photoageingmentioning

confidence: 71%

Elastin structure and its involvement in skin photoageing

Weihermann

Lorencini

Brohem

et al. 2016

Intern J of Cosmetic Sci

139

102

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“…Another prominent feature of photoaged skin is solar elastosis. 10 Some studies showed that solar elastosis could relate with increased tropoelastin gene expression 11 or an increased expression of fibrillin in the photodamaged skin. 12 In addition, degradation of elastic fibers by UV-induced MMP-12 might contribute to the pathology.…”

Section: Discussionmentioning

confidence: 99%

“…However, it may be related to the increased production of elastic fibers, which consist of elastin and fibrillin-rich microfibrils. 11,12 The degradation of elastin by MMP-12 (human macrophage metalloelastase) may also contribute to the development of solar elastosis in photoaged skin. 13 In the present study, we examined pre-and post-ALA-PDT specimens for AK and determined whether ALA-PDT induced histologic changes reversing the destructive connective tissue events associated with photoaging.…”

Section: Introductionmentioning

confidence: 99%

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Park

Sohn

Lee

et al. 2010

Journal of the American Academy of Dermatology

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Background: Repeated exposure to ultraviolet radiation from the sun results in premature photoaging. Photodynamic therapy (PDT) has been shown to be effective for treatment of photoaging, although the data from most studies have been based on clinical observation.Objectives: We investigated whether 5-aminolevulinic acid (ALA)-PDT induced histologic changes suggesting photorejuvenation.Methods: Fourteen patients with one to three actinic keratoses on the face were treated twice with ALA-PDT by using a 1200 W metal halogen lamp at 1-month intervals. Skin biopsy before and 1 month after the PDT was performed. Twenty-five pairs of specimens were obtained. We examined the specimens with routine and immunohistochemical staining and evaluated the parameters associated with photoaging by using image analysis.Results: After ALA-PDT, the mean epidermal thickness and dermal inflammatory infiltrate were reduced. The total collagen volume in the dermis significantly increased with expression of type I and III procollagen. The level of transforming growth factor b and transforming growth factor b type II receptors in the epidermis also increased. The elastotic material with co-localizing fibrillin-1 and tropoelastin expression in the dermis decreased after treatment. The expression of matrix metalloproteinases-1, -3, and -12 also decreased.Limitations: The study was limited by the small sample size.Conclusions: ALA-PDT resulted in histological changes indicating restoration of photoaged skin. These data suggest that ALA-PDT could be effective for photorejuvenation. ( J Am Acad Dermatol 2010;62:85-95.)

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“…[17][18][19] Reported in both in vivo and in vitro, UVB irradiation induces tropoelastin gene and protein expression in fibroblasts and keratinocytes, resulting in an aberrant accumulation of dermal elastic fibers and elastin content. 5,8,10,11,20 On the other hand, it has been reported that the degeneration of these fibers in chronologically and/or photoaged skin stems from an increase in matrix metalloproteinase-12 (MMP-12) and/or elastase, an elastin-degrading enzyme that is produced and secreted by dermal fibroblasts. [21][22][23][24] To date, the mechanisms underlying the alteration of elastin fibers in photoaged skin including their production, accumulation, and degradation are not fully understood.…”

mentioning

confidence: 99%

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Hachiya

Sriwiriyanont

Fujimura

et al. 2009

The American Journal of Pathology

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UVB irradiation has been reported to induce photoaging and suppress systemic immune function that could lead to photocarcinogenesis. However, because of the paucity of an UVB-induced photodamaged skin model, precise and temporal mechanism(s) underlying the deleterious effects of long-term UVB exposure on human skin have yet to be delineated. In this study, we established a model using human skin xenografted onto severe combined immunodeficient mice, which were subsequently challenged by repeated UVB irradiation for 6 weeks. Three-dimensional optical image analysis of skin replicas and noninvasive biophysical measurements illustrated a significant increase in skin surface roughness, similar to premature photoaging, and a significant loss of skin elasticity after long-term UVB exposure. Resembling authentically aged skin, UVB-exposed samples exhibited significant increases in epithelial keratins (K6, K16, K17), elastins, and matrix metalloproteinases (MMP-1, MMP-9, MMP-12) as well as degradation of collagens (I, IV, VII). The UVB-induced deterioration of fibrous keratin intermediate filaments was also observed in the stratum corneum. Additionally, similarities in gene expression patterns between our model and chronologically aged skin substantiated the plausible relationship between photodamage and chronological age. Furthermore , severe skin photodamage was observed when neutralizing antibodies against TIMP-1 , an endogenous inhibitor of MMPs , were administered during the UVB exposure regimen. Taken together , these findings suggest that our skin xenograft model recapitulates premature photoaged skin and provides a comprehensive tool with which to assess the deleterious effects of UVB irradiation.

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Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo

Cited by 91 publications

References 21 publications

Elastin structure and its involvement in skin photoageing

Elastin structure and its involvement in skin photoageing

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

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