Ultraviolet radiation at Mediterranean latitudes and protection efficacy of intraocular lenses

Artigas, José María Gatell; Felipe, Adelina; Artigas, Cristina; Menezo, J.L.

doi:10.1016/j.jfo.2012.03.018

Cited by 5 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ocular surface is continuously exposed to sunlight UV depending on geographical position, climate, lifestyle, and working habits [ 1 , 2 , 3 , 32 , 33 , 34 ]. Since UV rays can end at the ocular surface, the conjunctiva might be an elective target for an undesired development of inflammation and/or tissue remodeling in predisposed subjects ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…Some studies reported the modulation of few pterygium-related genes in UV-exposed FBs, reinforcing the link between UV and initiation and/or progression of human pterygium and/or exacerbation of preexisting fibrosis [ 6 , 53 ]. In addition, ocular surface discomfort might also occur in the case of light (UV) reflected from snow, water, and sand [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nerve Growth Factor (NGF) as Partaker in the Modulation of UV-Response in Cultured Human Conjunctival Fibroblasts

Esposito

Balzamino

Rocco

et al. 2022

IJMS

View full text Add to dashboard Cite

Corroborating data sustain the pleiotropic effect of nerve growth factor (NGF) in the protection of the visual system from dangerous stimuli, including ultraviolet (UV). Since UV exposure might promote ocular surface changes (conjunctival inflammation and matrix rearrangement), as previously reported from in vivo studies sustaining some protective NGF effects, in vitro cultures of human conjunctival fibroblasts (FBs) were developed and exposed to a single UV exposure over 15 min (0.277 W/m2), either alone or supplemented with NGF (1–10–100 ng/mL). Conditioned media and cell monolayers were collected and analyzed for protein release (ELISA, ELLA microfluidic) and transcript expression (real-time PCR). A specific “inflammatory to remodeling” pattern (IL8, VEGF, IL33, OPN, and CYR61) as well as a few epigenetic transcripts (known as modulator of cell differentiation and matrix-remodeling (DNMT3a, HDAC1, NRF2 and KEAP1)) were investigated in parallel. UV-exposed FBs (i), showed no proliferation or significant cytoskeleton rearrangement; (ii), displayed a trkANGFR/p75NTR phenotype; and (iii), synthesized/released IL8, VEGF-A, IL33, OPN, and CYR61, as compared to unexposed ones. NGF addition counteracted IL8, IL33, OPN, and CYR61 protein release merely at lower NGF concentrations but not VEGF. NGF supplementation did not affect DNMT3a or HDAC1 transcripts, while it significantly upregulated NRF2 at lowest NGF doses and did not change KEAP1 expression. Taken together, a single UV exposure activated conjunctival FBs to release pro-inflammatory/fibrogenic factors in association with epigenetic changes. The effects were selectively counteracted by NGF supplementation in a dose-dependent fashion, most probably accountable to the trkANGFR/p75NTR phenotype. Further in vitro studies are underway to better understand this additional NGF pleiotropic effect. Since UV-shield impairments represent a worldwide alert and UV radiation can slowly affect ocular surface homeostasis (photo-ageing, cataract) or might exacerbate ocular diseases with a preexisting fibrosis (pterygium, VKC), these findings on NGF modulation of UV-exposed FBs might provide additional information for protecting the ocular surface (homeostasis) from low-grade long-lasting UV insults.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nerve Growth Factor (NGF) as Partaker in the Modulation of UV-Response in Cultured Human Conjunctival Fibroblasts

Esposito

Balzamino

Rocco

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…In fact, the integrating sphere mode [14][15][16] is always used for ascertaining not only the shape of the spectral transmission curve, but also the total amount of light that passes through the IOL. The integrating sphere mode is also used for human 17 and pig 18 crystalline lenses.…”

Section: Discussionmentioning

confidence: 99%

Determination of scattering in intraocular lenses by spectrophotometric measurements

et al. 2014

View full text Add to dashboard Cite

Abstract. This study presents a method for measuring scattering in explanted intraocular lenses (IOLs). Currently, determining scattering in IOLs is usually performed by Scheimpflug cameras and the results are expressed in the units used by this apparatus. The method we propose uses a spectrophotometer and this makes it possible to measure the total transmission of the IOL by using an integrating sphere; the direct transmission is determined by the double-beam mode. The difference between these two transmissions gives a value of the scattering in percentage values of light lost. In addition, by obtaining the spectral transmission curve, information about the most scattered wavelengths is also obtained. The IOL power introduces errors when directly measured, particularly with high powers. This problem can be overcome if a tailor-made cuvette is used that shortens the distance between the IOL and the condensing lens of the spectrophotometer when the IOL powers are below 24 diopters. We checked the effectiveness of this method by measuring the scattering of three explanted IOLs from cornea donors. This method, however, does not make it possible to ascertain whether the scattering measured is caused by surface light scattering or internal light scattering. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…For spectral transmission measurement, we used a method described in previous studies (1,(12)(13)(14)(15) . The transmission curves were obtained using a Perkin-Elmer Lambda 35 UV/VIS spectrometer (Perkin-Elmer Inc., Shelton, CT, USA) ( Figure 1A).…”

Section: Spectral Transmission Measurementsmentioning

confidence: 99%

The influence of storage method on the transparency of pig crystalline lens

Artigas¹,

Fandiño²,

Navea³

et al. 2019

Arquivos Brasileiros De Oftalmologia

View full text Add to dashboard Cite

Ultraviolet radiation at Mediterranean latitudes and protection efficacy of intraocular lenses

Cited by 5 publications

References 26 publications

Nerve Growth Factor (NGF) as Partaker in the Modulation of UV-Response in Cultured Human Conjunctival Fibroblasts

Nerve Growth Factor (NGF) as Partaker in the Modulation of UV-Response in Cultured Human Conjunctival Fibroblasts

Determination of scattering in intraocular lenses by spectrophotometric measurements

The influence of storage method on the transparency of pig crystalline lens

Contact Info

Product

Resources

About