Ultraviolet Light Treatment of Titanium Enhances Attachment, Adhesion, and Retention of Human Oral Epithelial Cells via Decarbonization

Nakhaei, Kourosh; Ishijima, Manabu; Ikeda, Takayuki; Ghassemi, Amirreza; Saruta, Juri; Ogawa, Takahiro

doi:10.3390/ma14010151

Cited by 25 publications

(29 citation statements)

References 49 publications

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“…UV-treated titanium can collect or attract host-derived proteins on its surface, which results in enhanced bioactivity for both osteoblasts and fibroblasts. In addition, the attachment of gingival epithelial cells is enhanced on UV-treated titanium surfaces [124]. Taken together with the present study, soft tissue sealing could be enhanced by UV treatment.…”

Section: Discussionsupporting

confidence: 83%

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ikeda

Ueno

Saruta

et al. 2021

IJMS

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Peri-implantitis is an unsolved but critical problem with dental implants. It is postulated that creating a seal of gingival soft tissue around the implant neck is key to preventing peri-implantitis. The objective of this study was to determine the effect of UV surface treatment of titanium disks on the adhesion strength and retention time of oral connective tissues as well as on the adherence of mucosal fibroblasts. Titanium disks with a smooth machined surface were prepared and treated with UV light for 15 min. Keratinized mucosal tissue sections (3 × 3 mm) from rat palates were incubated for 24 h on the titanium disks. The adhered tissue sections were then mechanically detached by agitating the culture dishes. The tissue sections remained adherent for significantly longer (15.5 h) on the UV-treated disks than on the untreated control disks (7.5 h). A total of 94% of the tissue sections were adherent for 5 h or longer on the UV-treated disks, whereas only 50% of the sections remained on the control disks for 5 h. The adhesion strength of the tissue sections to the titanium disks, as measured by tensile testing, was six times greater after UV treatment. In the culture studies, mucosal fibroblasts extracted from rat palates were attached to titanium disks by incubating for 24, 48, or 96 h. The number of attached cells was consistently 15–30% greater on the UV-treated disks than on the control disks. The cells were then subjected to mechanical or chemical (trypsinization) detachment. After mechanical detachment, the residual cell rates on the UV-treated surfaces after 24 and 48 h of incubation were 35% and 25% higher, respectively, than those on the control surfaces. The remaining rate after chemical detachment was 74% on the control surface and 88% on the UV-treated surface for the cells cultured for 48 h. These trends were also confirmed in mouse embryonic fibroblasts, with an intense expression of vinculin, a focal adhesion protein, on the UV-treated disks even after detachment. The UV-treated titanium was superhydrophilic, whereas the control titanium was hydrophobic. X-ray photoelectron spectroscopy (XPS) chemical analysis revealed that the amount of carbon at the surface was significantly reduced after UV treatment, while the amount of TiOH molecules was increased. These ex vivo and in vitro results indicate that the UV treatment of titanium increases the adhesion and retention of oral mucosa connective tissue as a result of increased resistance of constituent fibroblasts against exogenous detachment, both mechanically and chemically, as well as UV-induced physicochemical changes of the titanium surface.

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Section: Discussionsupporting

confidence: 83%

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ikeda

Ueno

Saruta

et al. 2021

IJMS

Self Cite

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“…Previous studies have reported that neutrophils, T cells, and leukocytes are implicated in the in ammatory response and immune escape process of periodontitis [36,37]. Attachment, adhesion and retention of primary human gingival broblasts and microvascular formation are the main healing processes of periodontitis against microbial damage [38][39][40][41]. The level of serum IgG and salivary crossreactive IgG in patients with periodontitis is signi cantly high and is correlated with the severity of periodontitis [42].…”

Section: Discussionmentioning

confidence: 99%

CXCR4 and RAC2 as Biomarkers of Periodontitis and Their Potential Influence on Macrophage Infiltration

Tian

Zheng

Wang

et al. 2022

Preprint

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Objective Chronic periodontitis is an incurable infectious disease characterized by complex inflammatory immune response. This study aimed was to screen and identify effective biomarkers correlated with macrophage in chronic periodontitis. Materials and methods Periodontitis datasets obtained from the gene expression omnibus (GEO) were analyzed to identify differentially expressed genes (DEGs). Functional enrichment analysis of DEGs by GO and KEGG revealed multiple biological processes and signaling pathways related to immune response. Further analysis was performed on two immune-related gene databases, ImmPort and innateDB, to identify DEGs related to immune cells infiltration in chronic periodontitis. Subsequently, Protein-protein interaction (PPI) network were established to reveal hub genes with MCC algorithm using CytoHubba tool. Enrichment analysis in DisGeNet showed that immune-related DEGs were enriched in periodontitis. Next, CIBERSORTx tool was analyzed to investigate immune cells infiltration in GSE10334 dataset. Results The results demonstrated differential macrophage infiltration in periodontitis. Notably, RAC2 and CXCR4 were identified as biomarkers correlated with M2 macrophages in chronic periodontitis. Finally, receiver operating curve (ROC) was plotted to determine the diagnostic value of identified hub genes which was then validated using relevant datasets of macrophages. Conclusion RAC2 and CXCR4 are potential biomarker of chronic periodontitis correlated with M2 macrophages infiltration. Further experiments are needed to more deeply explore the roles of CXCR4 and RAC2 in the molecular mechanism of M2 macrophages in periodontitis microenvironment.

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“…Therefore, both null hypotheses were rejected. The UV photofunctionalization might help replace the HF as a decontaminant agent, as demonstrated in implant and miniscrew surfaces [ 16 , 22 , 33 ]. Furthermore, due to the indirect restorations' occlusal adjustment and saliva contamination during try-in [ 34 , 35 ], it is expected that dentists would return the restorations to the dental laboratory for repolishing of outer surfaces [ 36 ] and sandblasting of inner surfaces [ 34 – 36 ].…”

Section: Discussionmentioning

confidence: 99%

UV-Mediated Photofunctionalization of Indirect Restorative Materials Enhances Bonding to a Resin-Based Luting Agent

Ishikawa

Yamauti

Tichý

et al. 2021

BioMed Research International

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Purpose. The potential of UV-mediated photofunctionalization to enhance the resin-based luting agent bonding performance to aged materials was investigated. Methods. Sixty samples of each material were prepared. Yttria-stabilized zirconia (YZr) and Pd-Au alloy (Pd-Au) plates were fabricated and sandblasted. Lithium disilicate glass-ceramic (LDS) was CAD-CAM prepared and ground with #800 SiC paper. Half of the specimens were immersed in machine oil for 24 h to simulate the carbon adsorption. Then, all of the specimens (noncarbon- and carbon-adsorbed) were submitted to UV-mediated photofunctionalization with a 15 W UV-LED (265 nm, 300 mA, 7692 μW/cm2) for 0 (control groups), 5, and 15 min and subjected to contact angle (Ɵ) measurement and bonded using a resin cement (Panavia™ V5, Kuraray Noritake, Japan). The tensile bond strength (TBS) test was performed after 24 h. The Ɵ (°) and TBS (MPa) data were statistically analyzed using two-way ANOVA and Bonferroni correction tests ( α = 0.05 ). Results. In the carbon-adsorbed groups, UV-mediated photofunctionalization for 5 min significantly decreased Ɵ of all materials and increased TBS of YZr, and UV for 15 min significantly increased the TBS of LDS and Pd-Au. In noncarbon-adsorbed groups, UV-photofunctionalization did not significantly change the Ɵ or TBS except YZr specimens UV-photofunctionalized for 15 min. Conclusion. UV-mediated photofunctionalization might have removed the adsorbed hydrocarbon molecules from the materials’ surfaces and enhanced bond strengths of Panavia™ V5 to YZr, LDS, and Pd-Au. Additionally, UV-mediated photofunctionalization improved the overall TBS of YZr. Further investigation on the optimum conditions of UV photofunctionalization on indirect restorative materials should be conducted.

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Ultraviolet Light Treatment of Titanium Enhances Attachment, Adhesion, and Retention of Human Oral Epithelial Cells via Decarbonization

Cited by 25 publications

References 49 publications

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

CXCR4 and RAC2 as Biomarkers of Periodontitis and Their Potential Influence on Macrophage Infiltration

UV-Mediated Photofunctionalization of Indirect Restorative Materials Enhances Bonding to a Resin-Based Luting Agent

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