Ultraviolet light, mitomycin C and nitrous acid induce genetic instability in Streptomyces ambofaciens ATCC23877

Volff, Jean‐Nicolas; Vandewiele, Dominique; Simonet, Jeam-Marc; Decaris, Bernard

doi:10.1016/0027-5107(93)90008-4

Cited by 31 publications

(39 citation statements)

References 36 publications

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“…Colonies found for the latter type of progeny were of varying sizes, and in some cases they harbored aerial mycelium deficiencies, which differed between colonies. This phenomenon, named hypervariability, has been described in the spontaneous genetic instability of S. ambofaciens NSA2002 (32) and also in the strain DSM40697 (14). Seventeen Pig-mutants derived from the 17 sectors were restreaked on antibiotic-free medium.…”

Section: Methodsmentioning

confidence: 99%

“…In most mutants arising because of genetic instability, the ability to differentiate is often affected. For example, mutants produced at a high frequency in Streptomyces ambofaciens have altered abilities to produce pigment, and in some cases, antibiotics (physiological differentiation), and some mutants are unable to produce aerial mycelia, which prevents sporulation from occurring (morphological differentiation) (32). In addition, some characters related to primary metabolism can also be affected, such as prototrophy of some amino acids (15).…”

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confidence: 99%

“…In S. ambofaciens, UV, mitomycin C, and nitrous acid increase the frequency of pigment-negative (Pig-) mutants from 0.5% (spontaneous level) to more than 30% (32). Some DNA intercalating dyes such as ethidium bromide also possess this property (4).…”

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confidence: 99%

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Stimulation of genetic instability and associated large genomic rearrangements in Streptomyces ambofaciens by three fluoroquinolones

Volff

Vandewiele

Decaris

1994

Antimicrob Agents Chemother

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In Streptomyces ambofaciens NSA2002, pigmented wild-type colonies spontaneously give rise to pigmentnegative (Pig-) mutants at a frequency of about 0.5%. This genetic instability is related to large deletions which can be associated with amplifications of DNA sequences. The influence of three fluoroquinolones (ciprofloxacin, enoxacin, and norfloxacin) on this property was investigated. At a survival rate higher than 60%o, most colonies showed a patchwork phenotype consisting of phenotypically heterogeneous colonies harboring numerous mutant sectors. Moreover, the frequency of Pig-mutants rose to more than 90%o at survival rates equal to or higher than 10%0. Induced Pig-mutants showed the same phenotypical features as did spontaneous mutants. Most of them also harbored deletions, associated in some cases with DNA amplifications, in two loci of the large unstable region, AUD6 and AUD90 (derived from amplifiable unit of DNA). The size of deletions in induced mutants could rise to 1.5 Mb. These results show that ciprofloxacin, enoxacin, and norfloxacin greatly stimulate genetic instability and the occurrence of DNA rearrangements in S. ambofaciens. Moreover, these three fluoroquinolones had the same rank order for both toxic (i.e., antibacterial) and genotoxic activities. If the antibacterial effect of fluoroquinolones in S. ambofaciens is due to their interference with DNA gyrase, as shown for some other organisms, the genotoxic effect observed could be due to their interaction with this type II topoisomerase. This suggests that DNA gyrase is involved in the process of genetic instability in S. ambofaciens.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Stimulation of genetic instability and associated large genomic rearrangements in Streptomyces ambofaciens by three fluoroquinolones

Volff

Vandewiele

Decaris

1994

Antimicrob Agents Chemother

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“…One of the outstanding features of Streptomyces is its frequent chromosomal deletions (30,31), which could occur spontaneously or be induced by exposure to physical or chemical factors, such as UV radiation (32) and antibiotics (33). This phenomenon is believed to be related to the linearity of the chromosomes, and this genetic instability has not been shown to occur in circular chromosomes (7,20).…”

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Physical Map of the Linear Chromosome of Streptomyces hygroscopicus 10-22 Deduced by Analysis of Overlapping Large Chromosomal Deletions

et al. 2002

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The chromosomal DNA of Streptomyces hygroscopicus 10-22, a derivative of strain 5102-6, was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestions with AseI gave 11 fragments with a total length of ca. 7.36 Mb. The AseI sites were mapped by analysis of overlapping chromosomal deletions in different mutants and confirmed by Southern hybridizations using partially digested genome fragments and linking cosmids as probes. PFGE analysis of DNA with and without proteinase K treatment, together with the hybridization results, suggested a linear organization with terminal proteins and large terminal inverted repeats. Some deletion mutants had circular chromosomes.Streptomyces species are gram-positive, mycelium-forming soil bacteria with a high GϩC content (70 to 74%) in their DNA (5). They display a complex life cycle which involves the production of a large number of secondary metabolites, many of which are antibiotics. Pulsed-field gel electrophoresis (PFGE) allows the physical mapping of large bacterial chromosomes, and the physical maps of several Streptomyces chromosomes have been reported (11,16,17,21). Different approaches were used for mapping bacterial chromosomes (8). For example, hybridization of "linking clones" to Southern blots of restriction fragments separated by PFGE was used to establish the physical map of Streptomyces coelicolor A3(2) (11), Streptomyces lividans 66 (16), Streptomyces griseus (17), Streptomyces rimosus (21), and Saccharopolyspora erythraea (26). Physical maps were constructed for Mycoplasma mobile (2) and Pseudomonas aeruginosa (27) by employing one-and two-dimensional PFGE. A high-resolution physical map was established by ordering a set of cosmids in S. coelicolor A3(2) (25).One of the outstanding features of Streptomyces is its frequent chromosomal deletions (30, 31), which could occur spontaneously or be induced by exposure to physical or chemical factors, such as UV radiation (32) and antibiotics (33). This phenomenon is believed to be related to the linearity of the chromosomes, and this genetic instability has not been shown to occur in circular chromosomes (7,20).Streptomyces hygroscopicus 10-22 (37) can produce at least three useful antifungal antibiotics, one of which is used extensively and the others of which are used on a small scale in Chinese agriculture. Antibiotic 5102-I, an aminoglycoside similar to validamycin, is highly effective against Pellicularia sasakii, which causes rice sheath blight disease, Pellicularia filamentosa, which causes seedling blight of cotton, and Helminthosporium sigmoideum, which causes sclerotial disease of rice. Antibiotic II is a polypeptide useful for protection against leaf spot of corn caused by Cochlioobolus heterostrophus. Antibiotic III, whose structure is unknown, is useful for the control of cotton wilt caused by Fusarium oxysporum. Extensive efforts have therefore been devoted to identifying the compounds (35, 36), developing a gene cloning system (22), and improving th...

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“…DNA-damaging agents such as UV light stimulate the occurrence of rearrangements in Streptomyces (23). DNA lesions are removed in E. coli by excision.…”

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Influence of disruption of the recA gene on genetic instability and genome rearrangement in Streptomyces lividans

Volff

Altenbuchner

1997

J Bacteriol

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An enhancement of the deletion frequency was responsible for this mutator phenotype. The amplifiable locus AUD1 has a duplicated structure in some S. lividans strains and is frequently highly amplified in some mutants generated by genetic instability. The chromosomal AUD1 is not amplified in strain TK23 because of the lack of one duplication. Nevertheless, AUD1-derived amplifiable units presenting the typical duplicated organization amplified very well in TK23 when carried on a plasmid. No amplification of these units was observed in the recA mutant. The ability to amplify was restored when the wild-type recA gene was introduced into the plasmid carrying the amplifiable unit. These results suggest that the RecA protein plays a role in reducing the level of genetic instability and chromosomal deletions and show that the recA gene is necessary to achieve high-copy-number amplification of AUD1.Genetic instability in Streptomyces lividans is manifested by the occurrence of chloramphenicol-sensitive (Cml s

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Ultraviolet light, mitomycin C and nitrous acid induce genetic instability in Streptomyces ambofaciens ATCC23877

Cited by 31 publications

References 36 publications

Stimulation of genetic instability and associated large genomic rearrangements in Streptomyces ambofaciens by three fluoroquinolones

Stimulation of genetic instability and associated large genomic rearrangements in Streptomyces ambofaciens by three fluoroquinolones

Physical Map of the Linear Chromosome of Streptomyces hygroscopicus 10-22 Deduced by Analysis of Overlapping Large Chromosomal Deletions

Influence of disruption of the recA gene on genetic instability and genome rearrangement in Streptomyces lividans

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