Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation

Aita, Hideki; Att, Wael; Ueno, Takeshi; Yamada, Masahiro; Hori, Norio; Iwasa, Fuminori; Tsukimura, Naoki; Ogawa, Takahiro

doi:10.1016/j.actbio.2009.04.022

Cited by 155 publications

(210 citation statements)

References 33 publications

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“…Osteoblasts must adhere to titanium surfaces with various topographies depending on the device's structural and surface design. Taken together with the results of earlier studies on rough-surface titanium such as machined, acid-etched and sandblasted titanium [12][13][14][15][16], the present results obtained using very smooth, mirror-polished titanium surfaces indicate that UV-photofunctionalization technology can be universally applied to the bio-activation of titanium bulk material/devices, regardless of surface topography.…”

Section: Discussionmentioning

confidence: 54%

Section: Acta Biomatermentioning

confidence: 93%

“…Our previous culture study demonstrated that osteoblast adhesion is accelerated and enhanced on 6 Acta Biomater titanium surfaces, converting them from hydrophobic to hydrophilic [12,14,15]. Therefore, to evaluate the universality of the biological benefits of UV-photofunctionalization in terms of the osseointegration of implants and other such bone anchorage devices, it is necessary to determine whether UV-treatment enhances osoteoblastic adhesion to titanium surfaces regardless of surface topography.…”

Section: Acta Biomatermentioning

confidence: 99%

“…It was found that UV-treated titanium surfaces promoted cell migration, attachment and proliferation in osteoblastic cultures [12,14]. On UV-treated titanium, osteoblastic gene expression was also up-regulated and accelerated, but not as much as cell attachment and proliferation [12,14]. It was also shown that the in vitro expression of the osteoblastic phenotype was enhanced on UV-treated titanium, as evidenced by a marked increase in alkaline phosphatase activity and mineralized ECM formation compared to that on untreated titanium [12,15].…”

Section: Acta Biomatermentioning

confidence: 99%

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Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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Ultraviolet (UV)-photofunctionalization of titanium substantially enhances the strength and quality of osseointegration by promoting osteogenic cellular attachment and proliferation. However, the mechanism underlying the initial interaction between the cells and the surface of the material remains to be elucidated, especially where the influence of surface roughness is excluded as a factor.We evaluated the effect of UV-photofunctionalization on the adhesive strength and cellular stiffness of a single osteoblast and its association with extent of cell spread, cytoskeletal development and focal adhesion assembly on a very smooth titanium surface. Rat bone marrow-derived osteoblasts were cultured on UV-treated or -untreated mirror-polished titanium disks. The mean critical shear force required to initiate detachment of a single osteoblast (n = 10) was over 2000 nN on a UV-treated surface at 3 hr incubation, which was 17 times greater than that on an untreated surface.The mean total energy required to complete the detachment of osteoblasts (n = 10) was consistently over 60 pJ on a UV-treated titanium surface after 24 hr culture, which was up to 42 times greater than that on an untreated surface. Cellular shear modulus, which represents cellular stiffness, was consistently greater on a UV-treated surface than on an untreated surface after 24 hr incubation (n = 10). This strengthening of cell adhesion and cellular mechanical properties on UV-treated titanium was accompanied by enhanced cell spread and actin fiber development and increased levels of vinculin expression. These results indicate that UV-photofunctionalization substantially strengthens osteoblast retention on titanium bulk material with no topographical features and that this is associated with enhancement of intracellular structural development during the cell adhesion process.

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Section: Discussionmentioning

confidence: 54%

Section: Acta Biomatermentioning

confidence: 93%

Section: Acta Biomatermentioning

confidence: 99%

Section: Acta Biomatermentioning

confidence: 99%

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Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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“…Notwithstanding the rather well know surface chemistry of TiO 2 [3], some aspects related to UV exposure and the role of the chemical and biological properties of TiO 2 surfaces are still unclear. This is especially the case for TiO 2 nanostructures [19,20].…”

Section: Introductionmentioning

confidence: 99%

Selective binding of oligonucleotide on TiO 2 surfaces modified by swift heavy ion beam lithography

Pérez-Girón¹,

Hirtz²,

McAtamney³

et al. 2014

Nuclear Instruments and Methods in Physics Research Section B:

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We have used swift heavy-ion beam based lithography to create patterned bio-functional surfaces on rutile TiO 2 single crystals. The applied lithography method generates a permanent and well defined periodic structure of micrometre sized square holes having nanostructured TiO 2 surfaces, presenting different physical and chemical properties compared to the surrounding rutile single crystal surface. On the patterned substrates selective binding of oligonucleotides molecules is possible at the surfaces of the holes. This immobilisation process is only being controlled by UV light exposure. The patterned transparent substrates are compatible with fluorescence detection techniques, are mechanically robust, have a high tolerance to extreme chemical and temperature environments, and apparently do not degrade after ten cycles of use. These qualities make the patterned TiO 2 substrates useful for potential biosensor applications.

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Advanced Titanium Surfaces and Its Alloys for Orthopedic and Dental Applications Based on Digital SEM Imaging Analysis

Fadlallah

Ashour

Dey³

2016

Advanced Surface Engineering Materials

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Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation

Cited by 155 publications

References 33 publications

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Selective binding of oligonucleotide on TiO 2 surfaces modified by swift heavy ion beam lithography

Advanced Titanium Surfaces and Its Alloys for Orthopedic and Dental Applications Based on Digital SEM Imaging Analysis

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