Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging

Abstract

Ultraviolet (UV) fluorescence is a valuable tool for the imaging of a wide range of subjects. Like all imaging techniques, the key to success depends on the correct choice of equipment and approach used. In fluorescence photography, a filter is placed in front of the camera lens to block unwanted short-wavelength light from entering the camera, which would compromise the image. However, some filters exhibit fluorescence under UV light and can therefore have the potential to produce a color cast on the image. Filters also vary in how well they block unwanted light. A range of commonly used optical filters was assessed for fluorescence under UV light, and their optical transmission between 250 nm and 800 nm was measured. Finally, a simple method to enable the researcher to determine the fluorescence of the filters that they are using or wish to use for their work is described. The results indicate that the filters tested demonstrated a wide range of fluorescence under UV light and varying degrees of UV blocking. Some filters tested had equivalent or reduced fluorescence compared to Schott KV-418, which is a widely used, but, unfortunately, no longer manufactured UV blocking filter commonly used for fluorescence photography.

Applications of Ultraviolet and Sub-ultraviolet Dermatoscopy in Neoplastic and Non-neoplastic Dermatoses: A Systematic Review

“…These distinctions are in line with the terminology already used in photography (UV-induced fluorescence photography [ 3 – 6 ] and UV reflected/reflectance photography [ 7 , 8 ]. We intentionally added the “sUV/UV-induced” prefix to fluorescence dermatoscopy to emphasize that no UV signal is received by the eye.…”

Dual imaging technique for a real-time inspection system of foreign object detection in fresh-cut vegetables

“…By selectively transmitting the long wavelength emission signal and blocking excitation light, the long-pass filter enhances the contrast and visibility of fluorescence signals emitted by foreign objects against a background ( Yamazaki et al, 2012 ; Wei et al, 2013 ) developed a hand-held fluorescence imaging device that incorporates a long-pass thin-film interference filter to reject scattered excitation light, enabling the visualization of single nanoparticles and viruses. Crowther (2022) discussed using Quaser filters for ultraviolet fluorescence photography, similar to long-pass filters. Bueno et al (2014) emphasized the importance of dichroic mirrors and filters in color cameras to acquire fluorescence images effectively.…”

Ultraviolet Radiation Transmission in Buildings’ Fenestration: Part I, Detection Methods and Approaches Using Spectrophotometer and Radiometer

“…Spectrophotometers, on the other hand, are capable of measuring UV radiation at multiple wavelengths, allowing for a more comprehensive analysis. However, these instruments are often expensive and bulky, posing challenges to widespread use [5,[8][9][10][11][12][13][14].…”

“…Such advancements would enable the early identification of potential issues, facilitate timely repairs or replacements, and ensure the ongoing contribution of fenestration to optimal building energy performance. In this context, the burgeoning field of digital imaging technology, particularly digital image photography, offers a promising avenue for exploration and development, as will be detailed in Part II of this review [8][9][10].…”