Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria

Determann, S.; Lobbes, J. M.; Reuter, R.; Rullkötter, Jürgen

doi:10.1016/s0304-4203(98)00026-7

Cited by 201 publications

(117 citation statements)

References 29 publications

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“…The correlation between peak-T and biomass found here was also significant (R 2 5 0.36, p , 0.01), but lower than that between peak-M and biomass, indicating that the dynamics of protein-like substances are species specific. Determann et al (1998) observed the same pattern in nonaxenic algae cultures that included cells and exudates. They found that fluorescence intensities (Ex/Em 230 nm/330 nm) normalized to phytoplankton cell numbers were a function of the species and were also dependent on the physiological status.…”

Section: Discussionsupporting

confidence: 59%

Production of chromophoric dissolved organic matter by marine phytoplankton

Romera‐Castillo

Sarmento

Álvarez‐Salgado

et al. 2009

Limnology & Oceanography

259

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Incubation experiments with axenic cultures of four common phytoplankton species of the genera Chaetoceros, Skeletonema, Prorocentrum, and Micromonas were performed to test for the production of fluorescent dissolved organic matter (FDOM) by marine phytoplankton. Our results prove that the four species exuded both fluorescent protein-like and marine humic-like materials in variable amounts, with more production by the diatoms Chaetoceros sp. and Skeletonema costatum and less by Prorocentrum minimum. Whereas the exudation of protein-like substances by healthy phytoplankton cells has been recognized, the in situ production of marine humic-like substances is still a matter of debate. Using axenic cultures, we demonstrate unequivocally that phytoplankton can directly contribute to the autochthonous production of colored humic-like substances in the ocean. Extrapolation of these findings to the field indicates that about 20% of the marine humic-like substances produced in the highly productive coastal upwelling system of the Ría de Vigo could originate from growing phytoplankton. Therefore, the exudation of FDOM by marine phytoplankton should be considered in future studies of the dynamics of colored DOM in marine systems.

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Section: Discussionsupporting

confidence: 59%

Production of chromophoric dissolved organic matter by marine phytoplankton

Romera‐Castillo

Sarmento

Álvarez‐Salgado

et al. 2009

Limnology & Oceanography

259

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“…Both fractions have fluorescence properties that are similar to those already published for marine algae and bacterial cultures (Determann et al 1998). It is therefore likely that these components represent autochthonously derived protein material.…”

Section: Discussionsupporting

confidence: 74%

“…For example, the emission spectrum of human serum albumin resembles that of protein-bound tryptophan fluorescence only, despite the high molar ratio of tyrosine to tryptophan (18) and the high quantum yield of tyrosine (Lakowicz 1983). In general, the denaturation of proteins (containing tyrosine) leads to an increase in the observed fluorescence of tyrosine (Lakowicz 1983;Determann et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Tracing the production and degradation of autochthonous fractions of dissolved organic matter by fluorescence analysis

Stedmon¹,

Markager²

2005

Limnol. Oceanogr.

561

356

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We present the results of a mesocosm experiment investigating the production and utilization of autochthonous dissolved organic matter (DOM) by the plankton community under different inorganic nutrient regimes. Fluorescence spectroscopy combined with parallel factor analysis was applied to study the dynamics of autochthonous DOM. Seven independent fluorescent fractions were identified, differing in their spectral characteristics, production rates, and sensitivity to photochemical and microbial degradation processes. Five different humic fractions, a marine protein, and a peptide fluorescence were found. The five humic fractions were produced microbially, with the greatest production occurring under combined Si-and P-limiting conditions. The two proteinaceous fractions were produced during exponential growth of phytoplankton, irrespective of biomass composition. Photodegradation was an important sink for the microbially derived humic material, and the marine protein material was susceptible to both photoand microbial degradation.

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“…catechol) in the DOM (e.g. Determann et al, 1998). The presence of the tyrosine peak suggests the latter because tyrosine fluorescence is transferred to tryptophan if both amino acids are present (Yamashita and Tanoue, 2003).…”

Section: Group 1: Total Pc1 Subglacial Pc1 Marg Pc1 Alpine Streammentioning

confidence: 99%

Using synchronous fluorescence spectroscopy and principal components analysis to monitor dissolved organic matter dynamics in a glacier system

Barker

Sharp

Turner

2009

Hydrological Processes

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Abstract:The molecular characteristics of dissolved organic matter (DOM) reflect both its source material and its biogeochemical history. In glacial systems, DOM characteristics might be expected to change over the course of a melt season as changes in the glacier drainage system cause the mobilization of DOM from different OM pools. To test this hypothesis we used Principal Components Analysis (PCA) of synchronous fluorescence spectra to detect and describe changes in the DOM in meltwater from a glacier system in the Coast Mountains of northern British Columbia, Canada. For most of the melt season, the dominant component of subglacially routed meltwater DOM is characterized by a tyrosine-like fluorophore. This DOM component is most likely derived from supraglacial snowmelt. During periods of high discharge, a second component of DOM is present which is humic in character and similar to DOM sampled from a nearby non-glacial stream. This DOM component is inferred to be derived from a moss-covered soil environment that has been glacially overrun. It is probably entrained into glacial melt waters when the supraglacial meltwater flux exceeds the capacity of the principal subglacial drainage channels and water floods areas of the glacier bed that are normally isolated from the subglacial drainage system. Another source of DOM also appears to be mobilized during periods of high air temperatures. It is characterized by both humic and proteinaceous fluorophores and may be derived from the drainage of supraglacial cryoconite holes.

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Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria

Cited by 201 publications

References 29 publications

Production of chromophoric dissolved organic matter by marine phytoplankton

Production of chromophoric dissolved organic matter by marine phytoplankton

Tracing the production and degradation of autochthonous fractions of dissolved organic matter by fluorescence analysis

Using synchronous fluorescence spectroscopy and principal components analysis to monitor dissolved organic matter dynamics in a glacier system

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