Ultraviolet-C irradiation for inactivation of viruses in foetal bovine serum

Vaidya, Vivek; Dhere, Rajeev; Agnihotri, Snehal; Muley, Ravindra; Patil, Sanjay B.; Pawar, Amit

doi:10.1016/j.vaccine.2018.06.008

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…UV-based disinfection technique is profoundly used in the medical sectors to sterilize medical equipment, and PPEs ( Vaidya et al, 2018 ). UV(C) rays were proven to be more efficient than the UV(A) and UV(B) in inactivating viruses, as they possess high energy and are most absorbed by the DNA and RNA ( Ravanat et al, 2001 ).…”

Section: Inactivation Mechanismmentioning

confidence: 99%

A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance

Kumar

Mazumder

Mohapatra

et al. 2021

Journal of Hazardous Materials

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In this review, we present the environmental perspectives of the viruses and antiviral drugs related to SARS-CoV-2. The present review paper discusses occurrence, fate, transport, susceptibility, and inactivation mechanisms of viruses in the environment as well as environmental occurrence and fate of antiviral drugs, and prospects (prevalence and occurrence) of antiviral drug resistance (both antiviral drug resistant viruses and antiviral resistance in the human). During winter, the number of viral disease cases and environmental occurrence of antiviral drug surge due to various biotic and abiotic factors such as transmission pathways, human behaviour, susceptibility, and immunity as well as cold climatic conditions. Adsorption and persistence critically determine the fate and transport of viruses in the environment. Inactivation and disinfection of virus include UV, alcohol, chemical-base methods but the susceptibility of virus against these methods varies. Wastewater treatment plants (WWTPs) are major sources of antiviral drugs and their metabolites and transformation products. Ecotoxicity of antiviral drug residues against aquatic organisms have been reported, however more threatening is the development of antiviral resistance, both in humans and in wild animal reservoirs. In particular, emergence of antiviral drug-resistant viruses via exposure of wild animals to high loads of antiviral residues during the current pandemic needs further evaluation.

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Section: Inactivation Mechanismmentioning

confidence: 99%

A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance

Kumar

Mazumder

Mohapatra

et al. 2021

Journal of Hazardous Materials

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“…The manipulation with xenogeneic components and in particular, the common use of fetal bovine serum (FBS) as a growth factor supplement for cell culture, could increase the risk of immune reactivity in the host patient and exposure to viral, bacterial, or prion infection [ 10 , 11 ]. Immune reaction and diffuse urticariform development have been described in individuals who received several doses of MSCs produced with FBS [ 12 , 13 ]. Moreover, FBS is subjected to batch-to-batch variability, finally impacting on data reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells

et al. 2020

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Background The autologous nerve graft, despite its donor site morbidity and unpredictable functional recovery, continues to be the gold standard in peripheral nerve repair. Rodent research studies have shown promising results with cell transplantation of human adipose-derived stem cells (hADSC) in a bioengineered conduit, as an alternative strategy for nerve regeneration. To achieve meaningful clinical translation, cell therapy must comply with biosafety. Cell extraction and expansion methods that use animal-derived products, including enzymatic adipose tissue dissociation and the use of fetal bovine serum (FBS) as a culture medium supplement, have the potential for transmission of zoonotic infectious and immunogenicity. Human-platelet-lysate (hPL) serum has been used in recent years in human cell expansion, showing reliability in clinical applications. Methods We investigated whether hADSC can be routinely isolated and cultured in a completely xenogeneic-free way (using hPL culture medium supplement and avoiding collagenase digestion) without altering their physiology and stem properties. Outcomes in terms of stem marker expression (CD105, CD90, CD73) and the osteocyte/adipocyte differentiation capacity were compared with classical collagenase digestion and FBS-supplemented hADSC expansion. Results We found no significant differences between the two examined extraction and culture protocols in terms of cluster differentiation (CD) marker expression and stem cell plasticity, while hADSC in hPL showed a significantly higher proliferation rate when compared with the usual FBS-added medium. Considering the important key growth factors (particularly brain-derived growth factor (BDNF)) present in hPL, we investigated a possible neurogenic commitment of hADSC when cultured with hPL. Interestingly, hADSC cultured in hPL showed a statistically higher secretion of neurotrophic factors BDNF, glial cell-derived growth factor (GDNF), and nerve-derived growth factor (NFG) than FBS-cultured cells. When cocultured in the presence of primary neurons, hADSC which had been grown under hPL supplementation, showed significantly enhanced neurotrophic properties. Conclusions The hPL-supplement medium could improve cell proliferation and neurotropism while maintaining stable cell properties, showing effectiveness in clinical translation and significant potential in peripheral nerve research.

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“…Furthermore, the packed bed reactor outperforms the CFI and the JIB reactors of comparable scales in terms of RTD (Senčar, Hammerschmidt, Martins, & Jungbauer, 2020). Other systems have been suggested for continuous VI, however, some are not truly continuous in nature but rather cyclic (Gjoka et al, 2017;PALL, 2018) or do not consider the RTD when designing the incubation time (Arnold, Lee, Rucker-Pezzini, & Lee, 2019;Vaidya et al, 2018;Xenopoulos, 2013Xenopoulos, , 2015.…”

mentioning

confidence: 99%

Truly continuous low pH viral inactivation for biopharmaceutical process integration

Martins

Sencar

Hammerschmidt

et al. 2020

Biotech & Bioengineering

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Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous viral inactivation reactor (CVIR) with narrow residence time distribution (RTD) for low pH incubation. We show that the RTD does not broaden significantly over a wide range of linear flow velocities—which highlights the flexibility and robustness of the design. Prolonged exposure to acidic pH has no impact on bed stability, assuring constant RTD throughout long term operation. The suitability of the packed bed CVIR for low pH inactivation is shown with two industry‐standard model viruses, that is xenotropic murine leukemia virus and pseudorabies virus. Controls at neutral pH showed no system‐induced VI. At low pH, significant VI is observed, even after only 15 min. Based on the low pH inactivation kinetics, the continuous process is equivalent to traditional batch operation. This study establishes a concept for continuous low pH inactivation and, together with previous reports, highlights the versatility of the packed bed reactor for continuous VI, regardless of the inactivation method.

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Ultraviolet-C irradiation for inactivation of viruses in foetal bovine serum

Cited by 16 publications

References 20 publications

A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance

A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance

Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells

Truly continuous low pH viral inactivation for biopharmaceutical process integration

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