Ultraviolet B-induced MdWRKY72 expression promotes anthocyanin synthesis in apple

Hu, Jiafei; Fang, Hongcheng; Wang, Jie; Yue, Xuanxuan; Su, Mengyu; Mao, Zuolin; Qi, Zhang; Jiang, Huiyan; Guo, Zhangwen; Yu, Lei; Tian, Feng; Lu, Le; Peng, Zhenge; Zhang, Zongying; Wang, Nan; Chen, Xuesen

doi:10.1016/j.plantsci.2019.110377

Cited by 68 publications

(49 citation statements)

References 48 publications

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“…The development of different colors on the same variegated testa is a very interesting natural phenomenon despite they have the same genetic background. However, the molecular mechanism of pigment synthesis in the testa is still unclear.Plant anthocyanin metabolism is a complex process that included chalcone synthase (CHS), chalcone isomerase (CHI),dihydro avonnol-3-hydrogenase, (F3H),dihydro avonol-3'-hydrogenase(F3'H),dihydro avonol-3'-5'hydrogena,Dioxanonol-4-reductase (DFR), anthocyanidins synthase(ANS), and many other key enzymes 6 .Meanwhile, basic helix-loop-helix (bHLH), v-myb avian myeloblastosis viral oncogene homolog (MYB), WD40 protein, and other transcription factors can also regulate the expression of structural genes in the plant anthocyanin metabolic pathway 7 . The rst enzyme (CHS) in anthocyanin synthesis can affect the shade of plant color 8 and the color of the ower can change from purple to pink or even white if the CHS antisense gene is transformed in Petunia hybrida 9 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Hou

et al. 2020

Preprint

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Peanut (Arachis hypogaea L.) is one of the important oil and economic crops, among which the variegated testa peanut is a unique member. But the molecular mechanisms underlying the pigment synthesis in variegated testa are still unclear. Differentially expressed genes (DEGs) in pigment metabolism pathway in colored area indicated there were 27 DEGs highly related to the synthesis of variegated testa color among 1,050 DEGs,which were 13 up-regulated and 14 down-regulated,consisting of 3 PALs, 1 C4H, 2 CHSs, 1 F3H, 1 F3'H, 2 DFRs, 2 LARs, 2 IAAs, 4 bHLHs and 9 MYBs. GO analysis indicated DEGs were similarly enriched in 3 branches.KEGG analysis suggested flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. The liquid chromatography tandem mass spectrometry (LC-MS/MS) results showed that cyanidin and delphinidin were the main metabolites that caused the color difference between the colored area and the non-colored area. Through the verification of 20 DEGs via qPCR, the results were consistent with that of transcriptome sequencing in 4 comparison groups. The results in this study lay the foundation for revealing the molecular regulation mechanisms of anthocyanin synthesis in variegated testa peanut.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Hou

et al. 2020

Preprint

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“…The relationship between anthocyanin synthesis and UV-B radiation was recently studied by Hu et al (2020) in research on the expression of the transcription factor MdWRKY72 in apples, which is one of the factors attributed to the regulation of anthocyanin biosynthesis. The present study sought to elucidate some of the mechanisms of gene transcription that was stimulated by UV-B radiation applications, which decisively influenced the production of anthocyanins.…”

Section: Content Of Total Anthocyaninsmentioning

confidence: 99%

Impact of ultraviolet B radiation applications on some secondary metabolites in thyme (Thymus vulgaris L.)

Ramírez-Alvarado

Herrera

Rojas

et al. 2020

Rev. Colomb. Cienc. Hortic.

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The impact of ultraviolet radiation B (UV-B) applications on the production of secondary metabolites, such as phenols, flavonoids and anthocyanins, in thyme plants was studied. The stems, leaves and flowers were subjected to an experiment design that had a 4´2 factorial arrangement, evaluating: dose UV-B radiation (0.05 and 0.075 Wh m-2), sampling points (75 days after transplanting [cut-off point] and 92 days after transplanting [full flowering] according to the BBCH scale), adaptation time (24 and 49 hours) and extraction matrices of plant material (fresh and dried). The experiment unit corresponded to matrices from Thymus vulgaris L. (C.N. thyme) plants. Ten extractions were done per treatment, and a chemical analysis test were performed in triplicate. The extraction was done with a modified Randall method. The results showed that the application of UV-B radiation at a dose of 0.075 Wh m-2 increased the concentration of secondary metabolites of interest. The compounds that showed a better response to treatment were phenols and anthocyanins.

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“…However, the molecular mechanism of pigment synthesis in the testa is still unclear. Plant anthocyanin metabolism is a complex process that included chalcone synthase (CHS), chalcone isomerase (CHI), dihydroflavonol-3-hydrogenase (F3H), dihydroflavonol-3′-hydrogenase (F3′H), dihydroflavonol-3′-5′hydrogena, dioxanonol-4-reductase (DFR), anthocyanidins synthase (ANS), and many other key enzymes 4 . Meanwhile, basic helix-loop-helix (bHLH), v-myb avian myeloblastosis viral oncogene homolog (MYB), WD40 protein, and other transcription factors can also regulate the expression of structural genes in the plant anthocyanin metabolic pathway 5 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and metabolomic joint analysis reveals distinct flavonoid biosynthesis regulation for variegated testa color development in peanut (Arachis hypogaea L.)

Hou

et al. 2021

Sci Rep

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Peanut is one of the important oil and economic crops, among which the variegated testa peanut is a unique member. The molecular mechanisms underlying the pigment synthesis in variegated testa are still unclear. Differentially expressed genes (DEGs) in the flavonoid metabolism pathway in pigmented areas indicated that there were 27 DEGs highly related to the synthesis of variegated testa color among 1,050 DEGs. Of these 27, 13 were up-regulated and 14 were down-regulated, including 3 PALs, 1 C4H, 2 CHSs, 1 F3H, 1 F3'H, 2 DFRs, 2 LARs, 2 IAAs, 4 bHLHs, and 9 MYBs. GO (Gene Ontology) analysis indicated that DEGs were similarly enriched in three branches. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis suggested flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. The liquid chromatography–tandem mass spectrometry (LC–MS/MS) results showed that cyanidin and delphinidin were the primary metabolites that caused the color differences between the pigmented and the non-pigmented areas. Through the verification of 20 DEGs via qPCR, the results were consistent with transcriptome sequencing in four comparison groups. The results in this study lay the foundation for revealing the molecular regulation mechanisms of flavonoid synthesis in variegated testa peanut.

show abstract

Ultraviolet B-induced MdWRKY72 expression promotes anthocyanin synthesis in apple

Cited by 68 publications

References 48 publications

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Impact of ultraviolet B radiation applications on some secondary metabolites in thyme (Thymus vulgaris L.)

Transcriptomic and metabolomic joint analysis reveals distinct flavonoid biosynthesis regulation for variegated testa color development in peanut (Arachis hypogaea L.)

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